Neuromuscular Junction Damage in the Calf Muscles of Patients With Advanced Peripheral Artery Disease
Neuropathology and Applied Neurobiology,
Год журнала:
2025,
Номер
51(1)
Опубликована: Фев. 1, 2025
Peripheral
artery
disease
(PAD)
reduces
blood
flow
to
the
legs
and
causes
severe
muscle
leg
dysfunction
for
PAD
patients.
Skeletal
contractile
function
is
dependent
on
health
of
itself
that
neuromuscular
junction
(NMJ)
membrane.
To
determine
whether
NMJ,
including
motor
nerve
terminals
nicotinic
acetylcholine
receptors
(nAChR),
damaged
in
PAD,
gastrocnemius
muscles
were
collected
from
3
controls
13
patients
capture
images
331
control
NMJs
512
NMJs.
For
terminals,
there
more
denervated
nAChR
clusters
fewer
terminal
occupancies
patients,
compared
with
controls.
NMJs,
area
per
cluster
was
369.3
±
6.7
versus
225.2
5.3
μm2,
fragment
195.9
9.2
107.1
3.1
number
fragments
2.3
0.1
3.2
0.1,
endplate
75.7
1.6
55.7
1.1%,
total
distance
4.6
0.4
8.8
0.8
μm,
fragmented
7.6%
21.6%
respectively
(p
<
0.05
all
parameters).
Our
data
demonstrate
deterioration
clusters,
which
may
compromise
transmission,
contribute
observed
PAD.
Язык: Английский
Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
Translational research,
Год журнала:
2023,
Номер
260, С. 17 - 31
Опубликована: Май 21, 2023
Язык: Английский
The BALB/c.mdx62 mouse exhibits a dystrophic muscle pathology and is a model of Duchenne muscular dystrophy
Disease Models & Mechanisms,
Год журнала:
2024,
Номер
17(4)
Опубликована: Апрель 1, 2024
ABSTRACT
Duchenne
muscular
dystrophy
(DMD)
is
a
devastating
monogenic
skeletal
muscle-wasting
disorder.
Although
many
pharmacological
and
genetic
interventions
have
been
reported
in
preclinical
studies,
few
progressed
to
clinical
trials
with
meaningful
benefit.
Identifying
therapeutic
potential
can
be
limited
by
availability
of
suitable
mouse
models.
More
rigorous
testing
across
models
varied
background
strains
mutations
identify
treatments
for
success.
Here,
we
report
the
generation
DMD
model
CRISPR-induced
deletion
within
exon
62
dystrophin
gene
(Dmd)
first
generated
BALB/c
mice.
Analysis
mice
at
3,
6
12
months
age
confirmed
loss
expression
protein
isoform
Dp427
resultant
dystrophic
pathology
limb
muscles
diaphragm,
evidence
centrally
nucleated
fibers,
increased
inflammatory
markers
fibrosis,
progressive
decline
muscle
function,
compromised
trabecular
bone
development.
The
BALB/c.mdx62
novel
associated
variations
immune
response
phenotype,
compared
those
existing
It
represents
an
important
addition
toolbox
developing
strategies.
Язык: Английский
Comparison of Different Animal Models in Hindlimb Functional Recovery after Acute Limb Ischemia-Reperfusion Injury
Biomedicines,
Год журнала:
2024,
Номер
12(9), С. 2079 - 2079
Опубликована: Сен. 12, 2024
Acute
limb
ischemia
(ALI)
is
a
sudden
lack
of
blood
flow
to
limb,
primarily
caused
by
arterial
embolism
and
thrombosis.
Various
experimental
animal
models,
including
non-invasive
invasive
methods,
have
been
developed
successfully
used
induce
ischemia-reperfusion
injuries
(L-IRI).
However,
there
no
consensus
on
the
methodologies
in
models
for
L-IRI,
particularly
regarding
assessment
functional
recovery.
The
present
study
aims
compare
different
approaches
that
L-IRI
determine
optimal
model
In
this
study,
we
applied
pneumatic
cuff
as
method
ligated
aorta,
iliac,
or
femoral
artery
methods
L-IRI.
We
measured
grip
strength,
motor
function,
creatine
kinase
level,
inflammatory
markers
such
nuclear
factor
NF-κB,
interleukin-6
(IL-6),
hypoxia
hypoxia-induced
factor-1α
(HIF-1α),
evaluated
muscle
injury
with
hematoxylin
eosin
(H&E)
staining
Sprague
Dawley
rats
after
inducing
pressure
significantly
decreased
strength
rats,
causing
loss
ability
hold
grid
significant
function
impairment,
while
ligations
did
not.
conclude
from
tourniquet
could
be
ideal
studying
recovery
rat
model.
Язык: Английский