Extracellular symbiont colonizes insect during embryo development DOI Open Access
Miguel Ángel González Porras, Inès Pons, Marleny García-Lozano

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 5, 2023

Abstract Insects typically acquire their beneficial microbes early in development. Endosymbionts housed intracellularly are commonly integrated during oogenesis or embryogenesis, whereas extracellular only known to be acquired after hatching by immature instars such as larvae nymphs. Here, however, we report on an symbiont that colonises its host embryo Tortoise leaf beetles (Chrysomelidae: Cassidinae) digestive bacterial Stammera extracellularly within foregut symbiotic organs, and ovary-associated glands ensure vertical transmission. We outline the initial stages of colonization observe while organs develop three days prior larval emergence, they remain empty until final 24 hours Infection occurs timeframe, hatching. By experimentally manipulating availability embryos egg, describe a 12-hour developmental window governing . Symbiotic form normally aposymbiotic larvae, demonstrating these -bearing structures autonomously. In adults, already colonized following metamorphosis stable population facilitate folivory. The glands, initially lack Symbiont abundance subsequently increases transmission thereby ensuring sufficient titers at onset oviposition ∼29 metamorphosis. Collectively, our findings reveal precedes where proliferation is eventually decoupled adult match nutritional reproductive requirements host.

Язык: Английский

Hundreds of antimicrobial peptides create a selective barrier for insect gut symbionts DOI Creative Commons

Joy Lachat,

Gaëlle Lextrait,

Romain Jouan

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(25)

Опубликована: Июнь 12, 2024

The spatial organization of gut microbiota is crucial for the functioning ecosystem, although mechanisms that organize bacterial communities in microhabitats are only partially understood. insect Riptortus pedestris has a characteristic biogeography with multispecies community anterior midgut and monospecific population posterior midgut. We show region produces massively hundreds specific antimicrobial peptides (AMPs), Crypt-specific Cysteine-Rich (CCRs) have membrane-damaging activity against diverse bacteria but symbionts elevated resistance. determined by transposon-sequencing genetic repertoire symbiont Caballeronia insecticola to manage CCR stress, identifying different independent pathways, including AMP-resistance pathways unrelated known membrane homeostasis functions as well cell envelope functions. Mutants corresponding genes reduced capacity colonize midgut, demonstrating CCRs create selective barrier resistance symbionts. Moreover, once established gut, differentiate into CCR-sensitive state, suggesting second function peptide arsenal protecting epithelia or mediating metabolic exchanges between host Our study highlights evolution an extreme AMP family likely contributes establish control microbiota.

Язык: Английский

Процитировано

8
Extracellular symbiont colonizes insect during embryo development DOI Creative Commons
Miguel Ángel González Porras, Inès Pons, Marleny García-Lozano

и другие.

ISME Communications, Год журнала: 2024, Номер 4(1)

Опубликована: Янв. 1, 2024

Abstract Insects typically acquire their beneficial microbes early in development. Endosymbionts housed intracellularly are commonly integrated during oogenesis or embryogenesis, whereas extracellular only known to be acquired after hatching by immature instars such as larvae nymphs. Here, however, we report on an symbiont that colonizes its host embryo Tortoise beetles (Chrysomelidae: Cassidinae) digestive bacterial Stammera extracellularly within foregut symbiotic organs and ovary-associated glands ensure vertical transmission. We outline the initial stages of colonization observe although develop 3 days prior larval emergence, they remain empty until final 24 h Infection occurs timeframe hatching. By experimentally manipulating availability embryos egg, describe a 12-h developmental window governing Stammera. Symbiotic form normally aposymbiotic larvae, demonstrating these Stammera-bearing structures autonomously. In adults, already colonized following metamorphosis stable population facilitate folivory. The glands, initially lack Symbiont abundance subsequently increases transmission organs, thereby ensuring sufficient titers at onset oviposition ~29 metamorphosis. Collectively, our findings reveal precedes where proliferation is eventually decoupled adult match nutritional reproductive requirements host.

Язык: Английский

Процитировано

4
Peculiar structural features of midgut symbiotic organ in the early development of the stinkbug Plautia stali Scott, 1874 (Hemiptera: Pentatomidae) DOI
Toshiyuki Harumoto, Minoru Moriyama, Takema Fukatsu

и другие.

The Science of Nature, Год журнала: 2025, Номер 112(3)

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0
Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen DOI Creative Commons
Sandra Y. Mendiola, Jason Chen, Ben Lukubye

и другие.

Frontiers in Ecology and Evolution, Год журнала: 2024, Номер 12

Опубликована: Май 22, 2024

Many insects selectively associate with specific microbes in long-term, symbiotic relationships. Maintaining these associations can be vital for the insect hosts’ development, but must also contend potential coinfections from other environment. Fending off microbial threats while maintaining mutualistic has resulted many developing specialized organs to house beneficial microbes. Though locally concentrated organs, symbiont establishment have global consequences insect, including influence over success of coinfecting colonizing host. We use a transcriptomic approach examine how symbiosis between agricultural pest Anasa tristis and bacteria genus Caballeronia affects gene expression within host at large. simultaneously determine whether colonization impacts responses infection plant pathogen Serratia marcescens , which it vectors plants. found that no significant differential was elicited by S. marcescens. This surprising finding given previous work indicating A. clear rapidly compared aposymbiotic individuals. Our results indicate nonsymbiotic tissues differ greatly their expression, particularly following successful colonization. evidence local downregulation immunity upregulation cell communication functions facilitate - symbiosis.

Язык: Английский

Процитировано

2
Hundreds of antimicrobial peptides create a selective barrier for insect gut symbionts DOI Open Access

Joy Lachat,

Gaëlle Lextrait,

Romain Jouan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 16, 2023

Abstract The spatial organization of gut microbiota is crucial for the functioning ecosystem, although mechanisms that organize bacterial communities in microhabitats are only partially understood. insect Riptortus pedestris has a characteristic biogeography with multispecies community anterior midgut and mono-specific population posterior midgut. We show region produces massively hundreds specific antimicrobial peptides (AMPs), Crypt-specific Cysteine-Rich (CCRs) have membrane-damaging activity against diverse bacteria but symbionts elevated resistance. determined by transposon-sequencing genetic repertoire symbiont Caballeronia insecticola to manage CCR stress, identifying different independent pathways, including novel AMP-resistance pathways unrelated known membrane homeostasis functions as well cell envelope functions. Mutants corresponding genes reduced capacity colonize midgut, demonstrating CCRs create selective barrier resistance symbionts. Moreover, once established gut, differentiate into CCR-sensitive state, suggesting second function peptide arsenal protecting epithelia or mediating metabolic exchanges between host Our study highlights evolution an extreme AMP family contributes establish control microbiota.

Язык: Английский

Процитировано

6
The ncRNA-mediated regulatory networks of defensins and lysozymes in Riptortus pedestris: involvement in response to gut bacterial disturbances DOI Creative Commons
Yipeng Ren,

Siying Fu,

Wenhao Dong

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 17, 2024

Insects depend on humoral immunity against intruders through the secretion of antimicrobial peptides (AMPs) and immune effectors via NF-κB transcription factors, their fitness is improved by gut bacterial microbiota. Although there are growing numbers reports noncoding RNAs (ncRNAs) involving in responses pathogens, comprehensive studies ncRNA-AMP regulatory networks Riptortus pedestris , which one widely distributed pests East Asia, still not well understood under feeding environmental changes. The objective this study employed whole-transcriptome sequencing (WTS) to systematically identify lncRNAs (long RNA) circRNAs (circular obtain differential expression from R. different conditions. Functional annotation indicated that they were mainly enriched various biological processes with GO KEGG databases, especially signaling pathways. Five defensin (four novel members) eleven lysozyme (nine family genes identified characterized WTS data, meanwhile, phylogenetic analysis confirmed classification. Subsequently, miRNA–mRNA interaction network above two AMPs lncRNA-involved ceRNA (competing endogenous predicted built based bioinformatic prediction calculation, patterns differentially expressed (DE) defensins DE lysozymes related ncRNAs estimated selected among all comparison groups. Finally, integrate analyses previous 16S rRNA amplicon sequencing, we conducted Pearson correlation reveal significantly positive or negative between ncRNAs, as most changes microbiota at genus level . Taken together, present observations provide great insights into ncRNA response rearing insects uncover new potential strategies for pest control future.

Язык: Английский

Процитировано

1
Inferior Caballeronia symbiont lacks conserved symbiosis genes DOI Creative Commons
Katherine M. Martinez, Patrick T. Stillson, Alison Ravenscraft

и другие.

Microbial Genomics, Год журнала: 2024, Номер 10(12)

Опубликована: Дек. 16, 2024

Pentatomomorphan bugs can form symbiotic associations with bacteria belonging to the supergenus Burkholderia sensu lato . This relationship has become a model for understanding environmental symbiont acquisition. Host insects utilize various strains from across ; however, host colonization success and benefits conferred vary by bacterial clade. Therefore, we conducted meta-analysis aimed at identifying candidate genes that underpin beneficial symbioses within this system. We scanned entire Burkholderiaceae family presence of 17 colonization-associated genes, as well 88 are differentially expressed during symbiosis. There was no difference in distribution between ( Caballeronia insect-associated plant clade) non-symbiotic lineages; there higher prevalence insect lineages. subsequently analysed genomes nine species confer varying fitness their hosts. One significantly worse, one better remaining seven were intermediate terms benefits. found possessing number faster development time. Furthermore, identified two missing least but present other eight, suggesting these may be important modulating quality. Our study suggests mechanisms required broadly distributed Burkholderiaceae, determine quality more prevalent species. work helps identify influence highly specialized yet diverse symbiosis bacteria.

Язык: Английский

Процитировано

0
Extracellular symbiont colonizes insect during embryo development DOI Open Access
Miguel Ángel González Porras, Inès Pons, Marleny García-Lozano

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 5, 2023

Abstract Insects typically acquire their beneficial microbes early in development. Endosymbionts housed intracellularly are commonly integrated during oogenesis or embryogenesis, whereas extracellular only known to be acquired after hatching by immature instars such as larvae nymphs. Here, however, we report on an symbiont that colonises its host embryo Tortoise leaf beetles (Chrysomelidae: Cassidinae) digestive bacterial Stammera extracellularly within foregut symbiotic organs, and ovary-associated glands ensure vertical transmission. We outline the initial stages of colonization observe while organs develop three days prior larval emergence, they remain empty until final 24 hours Infection occurs timeframe, hatching. By experimentally manipulating availability embryos egg, describe a 12-hour developmental window governing . Symbiotic form normally aposymbiotic larvae, demonstrating these -bearing structures autonomously. In adults, already colonized following metamorphosis stable population facilitate folivory. The glands, initially lack Symbiont abundance subsequently increases transmission thereby ensuring sufficient titers at onset oviposition ∼29 metamorphosis. Collectively, our findings reveal precedes where proliferation is eventually decoupled adult match nutritional reproductive requirements host.

Язык: Английский

Процитировано

1