Journal of Comparative Pathology, Год журнала: 2022, Номер 197, С. 19 - 22
Опубликована: Авг. 11, 2022
Язык: Английский
International Journal of Obesity, Год журнала: 2024, Номер 48(8), С. 1080 - 1091
Опубликована: Март 19, 2024
Язык: Английский
Процитировано
3
Life Sciences, Год журнала: 2025, Номер 363, С. 123410 - 123410
Опубликована: Янв. 20, 2025
Язык: Английский
Процитировано
0
Journal of Nephrology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 30, 2025
Язык: Английский
Процитировано
0
Journal of Developmental Biology, Год журнала: 2023, Номер 11(1), С. 9 - 9
Опубликована: Фев. 20, 2023
Podocytes are exquisitely fashioned kidney cells that serve an essential role in the process of blood filtration. Congenital malformation or damage to podocytes has dire consequences and initiates a cascade pathological changes leading renal disease states known as podocytopathies. In addition, animal models have been integral discovering molecular pathways direct development podocytes. this review, we explore how researchers used zebrafish illuminate new insights about processes podocyte ontogeny, model podocytopathies, create opportunities discover future therapies.
Язык: Английский
Процитировано
7
Biomolecules, Год журнала: 2023, Номер 13(2), С. 266 - 266
Опубликована: Янв. 31, 2023
Glomerular parietal epithelial cells (PECs) have been increasingly recognized to crucial functions. Lineage tracking in animal models showed the expression of a podocyte phenotype by PECs during normal glomerular growth and after acute injury, suggesting reparative role PECs. Conversely, activated are speculated be pathogenic comprise extracapillary proliferation focal segmental glomerulosclerosis (FSGS) crescentic glomerulonephritis (CrescGN). The roles seem represent two sides PEC behavior directed local milieu mediators. Recent studies suggest microRNA-193a (miR193a) is involved pathogenesis FSGS CrescGN. In mouse model primary FSGS, induction miR193a caused downregulation Wilms' tumor protein, leading dedifferentiation podocytes. On other hand, inhibition resulted reduced crescent lesions Interestingly, vitro report that induces trans-differentiation into phenotype. This narrative review highlights critical health disease its modulation miR193a.
Язык: Английский
Процитировано
6
Science Translational Medicine, Год журнала: 2024, Номер 16(771)
Опубликована: Окт. 30, 2024
Cycles of a fasting-mimicking diet (FMD) promote regeneration and reduce damage in the pancreases, blood, guts, nervous systems mice, but their effect on kidney disease is unknown. In addition, FMD has not been tested rats. Here, we show that cycles newly developed low-salt (LS-FMD) restored normal proteinuria nephron structure function rats with puromycin-induced nephrosis compared animals renal did receive dietary intervention. LS-FMD induced modulation nephrogenic gene program, resembling developmental processes multiple structures. also activated podocyte-lineage reprogramming pathways promoted quiescent state mature podocytes rat model. pilot clinical study patients chronic disease, 5 days each month for 3 months renoprotection, including reduction improved endothelial function, who cycles. These results cycles, which cell types lead to glomerular reversal rats, should be further treatment progressive diseases.
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Май 30, 2024
Abstract Biological systems are complex, encompassing intertwined spatial, molecular and functional features. However, methodological constraints always limit the completeness of information that can be extracted. Here, we report development INSIHGT, a minimally perturbative, accessible cost-efficient three-dimensional (3D) spatial biology method utilizing superchaotropes host-guest chemistry. This allows highly multiplexed multi-modal readout tissue biomolecules in biological up to centimeter scales, permitting radio-histological correlation phosphorylated alpha-synuclein pathologies human hemi-brainstem. The homogeneous penetration permits reliable semi-quantitative signals 3D compared reference signals. Diverse antigens, mRNA transcripts, neurotransmitters, post-translational epigenetic modifications, well-preserved visualized. INSIHGT also multi-round probing for high-dimensional compatibility with downstream traditional histology. With mapped previously undescribed podocyte-to-parietal epithelial cell microfilaments demonstrated their geodesic clustering mouse glomeruli, catalogued sparsely located neurofilament-intensive inclusion bodies cerebellum, identified NPY-proximal types defined by morpho-proteomics hypothalamus. We anticipate form foundations multi-omics technology holistic studies.
Язык: Английский
Процитировано
1
Journal of the Egyptian National Cancer Institute, Год журнала: 2024, Номер 36(1)
Опубликована: Ноя. 18, 2024
Abstract Background Podocytopenia refers to a decrease in the number of podocytes. When podocytes are injured, they may detach leading podocytopenia, which represents critical step development podocytopathy and subsequently deterioration renal functions. Pathological assessment podocytopenia plays crucial role diagnosing underlying kidney diseases. Aim To assess detached evaluate their diagnostic focal segmental glomerulosclerosis. Materials methods This is retrospective study, conducted on 67 archival biopsies with clinical diagnosis steroid-resistant or steroid-dependent nephrotic syndrome (SRNS) diagnosed as glomerulosclerosis (FSGS) by electron microscopy (EM). Colloidal iron stain Desmin immunohistochemical were performed. Assessment mean percent stained pixels relation surface tuft area glomerulus, i.e., (PSA) was done using image analysis system (ImageJ 1.52a) software. Results Podocytopathy 35 (52.24%) cases, while FSGS 32 (47.76%) cases. Regarding podocytes, 27 (49.3%) cases showed no light (LM), only 4 (6%) severe podocyte detachment. There statistically significant difference between control both regarding PSA ( p ≤ 0.001). Conclusion Standardized reporting cells becoming mandatory have high positive predictive value for expected EM picture.
Язык: Английский
Процитировано
1
Journal of Diabetes, Год журнала: 2022, Номер 14(8), С. 514 - 523
Опубликована: Авг. 1, 2022
Abstract Diabetic kidney disease (DKD) is a severe microvascular complication of diabetes mellitus. It the leading inducement end‐stage renal (ESRD), and its global incidence has been increasing at an alarming rate. The strict control blood pressure glucose can delay progression DKD, but intensive treatment challenging to maintain. Studies date have failed find complete cure. glomerulus's alterations injuries play pivotal role in initiation development DKD. A wealth data indicates that interdependent relationship between resident cells glomerulus will provide clues mechanism DKD new ways for therapeutic intervention. This review summarizes significant findings glomerular cell cross talk focusing on cellular signaling pathways, regulators, potential novel avenues treating progressive
Язык: Английский
Процитировано
5
Science Advances, Год журнала: 2023, Номер 9(34)
Опубликована: Авг. 25, 2023
Members of the NSL histone acetyltransferase complex are involved in multiorgan developmental syndromes. While is known for its importance early development, role fully differentiated cells remains enigmatic. Using a kidney-specific model, we discovered that deletion members KANSL2 or KANSL3 postmitotic podocytes led to catastrophic kidney dysfunction. Systematic comparison two primary cell types reveals as master regulator intraciliary transport genes both dividing and nondividing cells. ablation loss cilia impaired sonic hedgehog pathway ciliated fibroblasts. By contrast, nonciliated responded with altered microtubule dynamics obliterated podocyte functions. Finally, overexpression wild-type but not double zinc finger (ZF-ZF) domain mutant rescued transcriptional defects, revealing critical function this assembly function. Thus, exhibits bifurcation functions enable diversity specialized outcomes
Язык: Английский
Процитировано
2