PIEZO Ion Channels in Cardiovascular Functions and Diseases

Circulation Research, Год журнала: 2024, Номер 134(5), С. 572 - 591

Опубликована: Фев. 29, 2024

The cardiovascular system provides blood supply throughout the body and as such is perpetually applying mechanical forces to cells tissues. Thus, this primed with mechanosensory structures that respond adapt changes in stimuli. Since their discovery 2010, PIEZO ion channels have dominated field of mechanobiology. These been proposed long-sought-after mechanosensitive excitatory involved touch proprioception mammals. However, more pieces evidence point importance activities disease development. channel-related cardiac functions include transducing hemodynamic endothelial vascular cells, red cell homeostasis, platelet aggregation, arterial pressure regulation, among others. contribute pathological conditions including hypertrophy pulmonary hypertension congenital syndromes generalized lymphatic dysplasia xerocytosis. In review, we highlight recent advances understanding role diseases. Achievements quickly expanding should open a new road for efficient control PIEZO-related diseases functions.

Язык: Английский

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Mechanical properties of the brain: Focus on the essential role of Piezo1‐mediated mechanotransduction in the CNS

Brain and Behavior, Год журнала: 2023, Номер 13(9)

Опубликована: Июнь 27, 2023

Abstract Background The brain is a highly mechanosensitive organ, and changes in the mechanical properties of tissue influence many physiological pathological processes. Piezo type ion channel component 1 (Piezo1), protein found metazoans, expressed involved sensing microenvironment. Numerous studies have shown that Piezo1‐mediated mechanotransduction closely related to glial cell activation neuronal function. However, precise role Piezo1 requires further elucidation. Objective This review first discusses roles regulating functions variety cells, then briefly assesses impact on progression dysfunctional disorders. Conclusions Mechanical signaling contributes significantly regulates processes such as differentiation, migration, axon guidance, neural regeneration, oligodendrocyte myelination. Additionally, plays significant normal aging injury, well development various diseases, including demyelinating Alzheimer's disease, tumors. Investigating pathophysiological mechanisms through which affects function will give us novel entry point for diagnosis treatment numerous diseases.

Язык: Английский

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Nociceptor mechanisms underlying pain and bone remodeling via orthodontic forces: toward no pain, big gain

Frontiers in Pain Research, Год журнала: 2024, Номер 5

Опубликована: Фев. 22, 2024

Orthodontic forces are strongly associated with pain, the primary complaint among patients wearing orthodontic braces. Compared to other side effects of treatment, pain is often overlooked, limited clinical management. lead inflammatory responses in periodontium, which triggers bone remodeling and eventually induces tooth movement. Mechanical subsequent inflammation periodontium activate sensitize periodontal nociceptors produce pain. Nociceptive afferents expressing transient receptor potential vanilloid subtype 1 (TRPV1) play central roles transducing nociceptive signals, leading transcriptional changes trigeminal ganglia. molecules, such as TRPV1, ankyrin 1, acid-sensing ion channel 3, P2X3 receptor, believed mediate Neuropeptides calcitonin gene-related peptides substance P can also regulate While transmit signals brain, they known modulate alveolar periodontitis. Therefore, molecules may contribute modulation movement, currently remains undetermined. Future studies needed better understand fundamental mechanisms underlying neuroskeletal interactions orthodontics improve treatment by developing novel methods reduce accelerate movement—thereby achieving “big gains no pain” orthodontics.

Язык: Английский

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Graph neural networks-based prediction of drug gene association of P2X receptors in periodontal pain

Journal of Oral Biology and Craniofacial Research, Год журнала: 2024, Номер 14(3), С. 335 - 338

Опубликована: Апрель 21, 2024

The P2X7 receptor, a member of the P2X receptor family, plays crucial role in various physiological processes, particularly pain perception. Its expression across immune, neuronal, and glial cells facilitates release pro-inflammatory molecules, thereby influencing development maintenance, as evidenced by its association with pulpitis rats. Notably, receptors such P2X3 are pivotal dental pathways, making them promising targets for novel analgesic interventions. Leveraging graph neural networks (GNNs) presents an innovative approach to model data, aiding identification drug prediction their efficacy, complementing advancements genomics proteomics therapeutic development. In this study, 921 drug-gene interactions involving were accessed through https://www.probes-drugs.org/. These underwent meticulous annotation, preprocessing, subsequent utilization train assess GNNs. Furthermore, leveraging Cytoscape, CytoHubba plugin, other bioinformatics tools, gene constructed pinpoint hub genes within these interactions. Through analysis, SLC6A3, SLC6A2, FGF1, GRK2, PLA2G2A identified central context receptor-mediated Despite achieving 65 percent accuracy rate, GNN demonstrated suboptimal predictive power gene-drug associated oral pain. Hence, further refinements enhancements imperative unlock full potential elucidating targeting pathways underlying mechanisms.

Язык: Английский

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Dentin Mechanobiology: Bridging the Gap between Architecture and Function

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 5642 - 5642

Опубликована: Май 22, 2024

It is remarkable how teeth maintain their healthy condition under exceptionally high levels of mechanical loading. This suggests the presence inherent adaptation mechanisms within structure to counter constant stress. Dentin, situated between enamel and pulp, plays a crucial role in mechanically supporting tooth function. Its intermediate stiffness viscoelastic properties, attributed its mineralized, nanofibrous extracellular matrix, provide flexibility, strength, rigidity, enabling it withstand loading without fracturing. Moreover, dentin’s unique architectural features, such as odontoblast processes dentinal tubules spatial compartmentalization odontoblasts dentin sensory neurons contribute distinctive perception external stimuli while acting defensive barrier for dentin-pulp complex. Since architecture governs functions nociception repair response stimuli, understanding mechanobiology developing treatments pain management dentin-associated diseases regeneration. review discusses physical features regulate mechano-sensing, focusing on mechano-sensitive ion channels. Additionally, we explore advanced vitro platforms that mimic providing deeper insights into fundamental mechanobiological phenomena laying groundwork effective mechano-therapeutic strategies diseases.

Язык: Английский

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Oral and maxillofacial mechanomedicine: Exploring biomechanical insights and their clinical implications

Translational dental research., Год журнала: 2025, Номер 1(1), С. 100008 - 100008

Опубликована: Янв. 1, 2025

Язык: Английский

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Pericytes Are Odontoblast Progenitor Cells Depending on ER Stress

Journal of Dental Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Odontoblasts are terminally differentiated cells that exhibit mechanosensitivity and mineralization capacity. Mechanosensitive ion channels such as Piezo1 present in odontoblasts associated with their physiological functions via Ca 2+ signaling. Both signals influx from mechanosensitive release stores function secondary messenger systems for various biological phenomena. The endoplasmic reticulum (ER) serves an intracellular store mobilizes . Changes concentration inside the ER among factors cause stress. Perivascular located around dental pulp. Although formation indicates perivascular interact odontoblasts, detailed profiles under developmental pathological conditions remain unclear. In this study, we revealed pericyte marker, neural/glial antigen 2 (NG2)–positive cells, cell-rich zones (CZs) can differentiate into Piezo1-positive following genetic odontoblast depletion mice, modeled death after severe dentin injury reparative formation. NG2-positive pericytes faster than glial cells. To determine how focused on ER-stress sensor protein, activating transcription factor 6a (ATF6a). After depletion, regenerated layer were capable of acting functional odontoblasts. presence extracellular , application a sarco/ER -ATPase (SERCA) inhibitor, thapsigargin, known inducer, increased lineage (OLCs). increase was significantly inhibited by pharmacologic indicating stress SERCA inhibition augmented Piezo1-induced responses progenitor However, activation G q -coupled receptors adenosine diphosphate did not induce activation. Gene silencing ATF6a and/or NG2 impaired OLCs. Overall, orchestrates differentiation act sensory receptor dentin-forming

Язык: Английский

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Sensory innervation of rat molar root pulp and dentin, apex, foramen, cellular cementum, vasculature and periodontium

Archives of Oral Biology, Год журнала: 2025, Номер 174, С. 106238 - 106238

Опубликована: Март 16, 2025

Trigeminal innervations of dental roots have only been partly investigated. The aim this study was to document sensory innervation rat molar roots, especially their neural patterns in periapical tissues that may affect tooth pain. nerve ending young and old molars had labeled by tritiated axonal-transported protein were compared immunocytochemistry for peripherin, calcitonin gene-related peptide, p75-neurotrophin receptor, synaptophysin, Substance P, neurofilament-200. Nine found the autoradiograms: (1) PERIAPICAL PULP large axons among odontoblasts, other with free endings pulp, plus dentin on palatal-lingual buccal sides each root. (2) CELLULAR CEMENTUM MATRIX (CCx) small channels extending across from periodontium apex, trigeminal CCx at its apical periodontal edges. (3) THE FORAMEN REGION many much vascular innervation. (4) APEX a variety along walls. (5) NEARBY PERIODONTIUM neurovascular clusters including Ruffini mechanoreceptors near foramen. Immunocytochemistry showed CGRP, receptor individual tissues, while P neurofilament more limited. Differences between lateral accessory canals noted. nine identified so far differ crown root shaft. They likely important effects pain general pains can be difficult anesthetize treat.

Язык: Английский

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Beyond hydrodynamics: The role of ion channels in dentine hypersensitivity

Journal of Dentistry, Год журнала: 2025, Номер unknown, С. 105745 - 105745

Опубликована: Апрель 1, 2025

Язык: Английский

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Pannexin 1 channels: A Bridge Between Synaptic Plasticity and Learning and Memory Processes.

Neuroscience & Biobehavioral Reviews, Год журнала: 2025, Номер unknown, С. 106173 - 106173

Опубликована: Апрель 1, 2025

Язык: Английский

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