Journal of Analytical Atomic Spectrometry,
Год журнала:
2023,
Номер
38(12), С. 2691 - 2702
Опубликована: Янв. 1, 2023
In
this
study,
we
conducted
an
extensive
computational
investigation
using
various
theoretical
approaches
to
elucidate
the
molecular-level
interactions
between
ten
representative
volatile
organic
compounds
and
functionalized
graphene
nanosheets.
Communications Medicine,
Год журнала:
2024,
Номер
4(1)
Опубликована: Дек. 28, 2024
Menopause
driven
decline
in
estrogen
exposes
women
to
risk
of
osteoporosis.
Detection
early
onset
and
silent
progression
are
keys
prevent
fractures
associated
burdens.
In
a
discovery
cohort
120
postmenopausal
women,
we
combined
repeated
quantitative
pulse-echo
ultrasonography
bone,
assessment
grip
strength
serum
bone
markers
with
mass-spectrometric
analysis
exhaled
metabolites
find
breath
volatile
cutoff
levels
for
Obtained
cutoffs
were
validated
an
independent
49
age-matched
six
months
apart
seasonal
follow-ups.
Here,
within
the
cohort,
concentrations
end-tidal
dimethyl
sulfide
(DMS),
allyl-methyl
sulfide,
butanethiol
butyric
acid
increased
(p
≤
0.005)
pronouncedly
subjects
mineral
density
(BMD)
at
high-risk
osteoporosis
fracture,
when
compared
normal
BMD.
Increased
age
decreased
concomitant.
All
changes
reproduced
during
validation
Exhaled
metabolite
expressions
remain
independent.
Serum
show
random
without
reproducibility.
DMS
exhalations
differs
between
patients
recent,
old
fractures.
Metabolite
BMDs
down-regulated
winter.
ROC
yields
high
classification
accuracy
osteoporosis,
which
predicts
>91%
sensitivity
specificity.
Non-invasive
allowed
more
reliable
than
conventional
markers.
We
identified
associations
organosulfur
short-chain
fatty
acids
metabolism
via
gut-bone
axis.
It
is
estimated
globally
that
one-third
aged
>50
years
experience
(breaks
their
bones)
from
(bone
weakening
brittleness).
difficult
diagnose
this
condition
makes
it
hard
put
place
measures
help
investigate
links
organic
chemicals
detectable
breath,
blood
(tested
by
measuring
strength).
discover
coming
gut
strongly
health.
Our
non-invasive
faster
standard
currently
used
diagnosing
identifies
connection
bones
not
previously
shown.
These
findings
offer
easier
paths
towards
new
therapeutic
targets.
Sukul
et
al.
analyze
endogenous
indicative
The
serves
as
rapid
includes
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 2, 2023
Abstract
Background
Due
to
their
immediate
exhalation
after
generation
at
the
cellular/microbiome
levels,
exhaled
volatile
organic
compounds
(VOCs)
may
provide
real-time
information
on
pathophysiological
mechanisms
and
host
response
infections.
In
recent
years,
metabolic
profiling
of
most
frequent
respiratory
infection
gained
interest
as
it
holds
potential
for
early
non-invasive
detection
pathogens
monitoring
disease
progression
therapy.
Methods
contrast
previous
studies
with
pre-selected
patient
groups,
we
conducted
a
mass-spectrometry
based
breath
in
hundreds
consecutive
subjects
under
an
actual
screening
scenario.
Recruited
were
grouped
further
comparisons,
multiplex-PCR
confirmed
(infected
by
common
pathogen(s)
healthy)
presence
or
absence
flu
like
symptoms.
Results
Amongst
recruitments,
obtained
256
healthy
cases
223
infected/coinfected
(171
mono-infections,
52
coinfections)
Haemophilus
influenza
,
Streptococcus
pneumoniae
Rhinovirus.
We
observed
multiple
effects
these
mono-infections
co-infections
onto
VOC
profiles
variations,
especially
endogenous
ketone,
short-chain
fatty
acid,
organosulfur,
aldehyde
terpene
concentrations.
Based
VOCs
origins,
encountered
changes
patient’s
energy
metabolism,
systemic
microbial
immune
homeostasis,
inflammation,
oxidative
stress
antioxidative
defense.
Presence
bacterial
depicted
more
complex
cross-talk
–
likely
due
own
metabolism.
Conclusion
Alike
our
reports
COVID-19
line
other
multi-omics
clinical
microbiological
reports,
results
offered
unique
insight
into
infections,
pathogenesis,
‘
host-microbiome-pathogen
’
interactions.
Breathomics
‘monitoring’
coinfections.
This
research
presents
two
laser-based
photoacoustic
approaches
for
analyzing
exhaled
breath
isoprene
and
acetone.
The
integration
of
a
PTR-ToF-MS
as
reference
device
ensured
the
reliability
accuracy
PAS
systems
that
is
based
on
an
ICL
QCL
acetone
detection.
calibration
yielded
limits
detection
26.9
ppbV
1.7
ppbV,
respectively,
corresponding
normalized
noise
equivalent
absorption
coefficients
(NNEAs)
5.0E-9
Wcm-1Hz-0.5
4.9E-9
Wcm-1Hz-0.5.
Laboratory
well
real
sample
measurements
from
alveolar
revealed
robust
system
performance,
with
only
one
outlier
within
static
measurements.
However,
discrepancies
emerged
under
dynamic
sampling
conditions,
emphasizing
need
further
optimization.
Especially
by
knowing
nature
endogenous
origin
our
findings
highlight
potential
analysis
non-invasive
physio-metabolic
pathophysiological
monitoring
towards
point-of-care
devices.
Applied Sciences,
Год журнала:
2024,
Номер
14(24), С. 11501 - 11501
Опубликована: Дек. 10, 2024
In
the
current
work,
non-invasive
approach
of
breath
analysis
is
implemented
for
first
time
in
an
eccentric
exercise
protocol
that
investigated
effect
spirulina
supplementation
on
exercise-induced
oxidative
stress.
We
assessed
whether
volatile
alkanes
exhaled
can
serve
as
alternative
biomarkers
A
randomized,
double-blinded,
placebo-controlled,
crossover
study
was
carried
out
enrolling
14
participants.
The
volunteers
consumed
42
mg·kg−1
body
weight
either
Spirulina
Nigrita®
or
maltodextrin,
a
placebo,
daily
15
days.
Afterward,
they
followed
damaging
upper
limbs.
Expired
samples
were
collected
from
them
just
before
(baseline
measurement),
prior
to
exercise,
and
1
h,
24
48
72
h
after
exercise.
analyzed
by
Gas
Chromatography/Mass
Spectrometry
(GC-MS)
coupled
with
thermal
desorption
unit
(TDU)
determine
alveolar
gradient
(AG)
several
alkanes,
C5–C14,
are
known
be
related
Apart
analysis,
TBARSs
also
determined
crude
marker
lipid
peroxidation.
Two-way
repeated
measures
ANOVA
tests
applied
alkanes’
AGs
between
(SPI)
placebo
(PL)
groups
across
time.
PL
group,
trend
increasing
almost
all
immediately
gradual
return
pre-exercise
levels
up
later
revealed.
statistically
significant
observed
2-methylhexane,
3-methylhexane,
heptane,
octane,
undecane.
administration
appeared
reduce
increases
attenuation
2-methylpentane
2-methylhexane.
An
examination
confirmed
reduced
SPI
group
due
changes
peroxidation,
while
positive
correlation
iAUC
2-methylhexane
3-methylhexane
conclusion,
may
attractive
assessing
redox
compared
traditional
blood
biomarkers.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 26, 2023
Abstract
Isoprene
is
amongst
the
most
abundant
volatile
metabolites
produced
by
plants
and
animals.
Human
exhaled
isoprene
related
to
various
pathophysiology
but
origin
was
yet
uncertain.
Among
2000
individuals
screened
within
consecutive
mass-spectrometric
studies,
five
healthy
adults
without
breath
were
identified.
Whole
exome
sequencing
in
these
revealed
a
shared
homozygous
(<
1%
prevalent)
IDI2
stop-gain
mutation
preventing
conversion
of
isopentenyl
diphosphate
dimethylallyl
(DMAPP)
cholesterol
metabolism.
Targeted
depicted
that
variant
heterozygous
deficient
blood-relatives
absent
unrelated
normal
adults.
Wildtype
IDI1
metabolism
serological
parameters
everyone.
determines
production
as
DMAPP
only
source
unlike
plants,
humans
lack
synthase
its
homologue.
expressed
skeletal-myocyte
peroxisomes
instant
spikes
exhalation
during
muscle
activity
confirm
from
muscular
lipolytic
Well-defined
endogenous
translated
clinically
interpretable
biomarker.
Journal of Analytical Atomic Spectrometry,
Год журнала:
2023,
Номер
38(12), С. 2691 - 2702
Опубликована: Янв. 1, 2023
In
this
study,
we
conducted
an
extensive
computational
investigation
using
various
theoretical
approaches
to
elucidate
the
molecular-level
interactions
between
ten
representative
volatile
organic
compounds
and
functionalized
graphene
nanosheets.