Local wakefulness‐like activity of layer 5 cortex under general anaesthesia DOI
Jesús Pardo‐Valencia, Miryam Moreno‐Gomez,

Noelia Mercado

и другие.

The Journal of Physiology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 24, 2024

Abstract Consciousness, defined as being aware of and responsive to one's surroundings, is characteristic normal waking life typically lost during sleep general anaesthesia. The traditional view consciousness a global brain state has evolved toward more sophisticated interplay between local states, with the presence in awake wakefulness sleeping brain. However, this not clear for anaesthesia, where loss was recently suggested be associated brain‐wide synchrony that selectively involves layer 5 cortical pyramidal neurons across sensory, motor associative areas. According view, cortex should incompatible deep hypothesis deserves scrutinised causal manipulations. Here, we show unilateral chemogenetic activation sensorimotor isoflurane‐anaesthetised mice induces transition from slow‐wave activity tonic firing transfected hemisphere. This wakefulness‐like dramatically disrupts interhemispheric mirror‐image locations contralateral hemisphere, but does reduce level unconsciousness under nor transitions to/from Global may thus sufficient condition anaesthesia‐induced unconsciousness, necessary one, at least isoflurane Local can induced maintained encouraging further investigation into vs . aspects unconsciousness. image Key points neural correlates have states nuanced evident terms brains brains. concept remains unclear been involve neurons. We found chemogenetically anaesthetised without affecting depth anaesthesia or feature by compatible promoting vs.

Язык: Английский

Near-Infrared II Photobiomodulation Preconditioning Ameliorates Stroke Injury via Phosphorylation of eNOS DOI
Shinya Yokomizo, Timo Kopp, Malte Roeßing

и другие.

Stroke, Год журнала: 2024, Номер 55(6), С. 1641 - 1649

Опубликована: Апрель 4, 2024

The current management of patients with stroke intravenous thrombolysis and endovascular thrombectomy is effective only when it timely performed on an appropriately selected but minor fraction patients. development novel adjunctive therapy highly desired to reduce morbidity mortality stroke. Since endothelial dysfunction implicated in the pathogenesis featured suppressed nitric oxide synthase (eNOS) concomitant deficiency, restoring represents a promising approach treating injury.

Язык: Английский

Процитировано

9
The impact of isoflurane anesthesia on brain metabolism in mice: An MRI and electroencephalography study DOI
Zhiliang Wei, Seung‐Eon Roh,

Xiuli Yang

и другие.

NMR in Biomedicine, Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract Isoflurane is one of the most widely used anesthetic agents in rodent imaging studies. However, impact isoflurane on brain metabolism has not been fully characterized to date, primarily due a scarcity noninvasive technologies quantitatively measure brain's metabolic rate vivo. In this study, using noncontrast MRI techniques, we dynamically measured cerebral oxygen (CMRO 2 ) under varying doses anesthesia mice. Concurrently, systemic parameters heart and respiration rates were recorded alongside CMRO . Additionally, electroencephalogram (EEG) recording was identify changes neuronal activities same regimen employed experiments. We found suppression by dose‐dependent manner, concomitant with diminished high‐frequency EEG activity. The degree strongly correlated rate, which offers potential approach calibrate measurements. Furthermore, level associated neural responses somatosensory motor cortices mice estimated as 308.2 μmol/100 g/min. These findings may facilitate integration into future studies involving animal disease models usage.

Язык: Английский

Процитировано

6
Discovery of a new long COVID mouse model via systemic histopathological comparison of SARS-CoV-2 intranasal and inhalation infection DOI Creative Commons

Donghun Jeon,

Sunghee Kim, Jiseon Kim

и другие.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2024, Номер 1870(7), С. 167347 - 167347

Опубликована: Июль 15, 2024

Intranasal infection is commonly used to establish a SARS-CoV-2 mouse model due its non-invasive procedures and minimal effect from the operation itself. However, mice intranasally infected with have high mortality rate, which limits utility of this for exploring therapeutic strategies sequelae non-fatal COVID-19 cases. To resolve these limitations, an aerosolised viral administration method has been suggested. in-depth pathological analysis comparing two models lacking. Here, we show that inhalation intranasal (10

Язык: Английский

Процитировано

3
Metabolic and vascular imaging markers for investigating Alzheimer’s disease complicated by sleep fragmentation in mice DOI Creative Commons
Xiaoning Han, Guanshu Liu, Sang‐Soo Lee

и другие.

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 20, 2024

Background Sleep problem is a common complication of Alzheimer’s disease (AD). Extensive preclinical studies have been performed to investigate the AD pathology. However, pathophysiological consequence complicated by sleep remains be further determined. Purpose To brain metabolism and perfusion in an mouse model problem, subsequently identify potential imaging markers better understand associated pathophysiology. Methods We examined oxygen extraction fraction (OEF), cerebral metabolic rate (CMRO 2 ), blood flow (CBF) using state-of-the-art MRI techniques cohort 5xFAD mice. Additionally, neuroinflammation, indicated activated microglia, was assessed histology techniques. fragmentation (SF) utilized as representative for problems. Results SF with significant increases OEF ( P = 0.023) CMRO 0.029), indicating state hypermetabolism. CBF showed genotype-by-sleep interaction effect 0.026), particularly deep regions such hippocampus thalamus. Neuroinflammation primarily driven genotype rather than SF, especially measurements. Conclusion These results suggest that measurements are promising studying co-pathogenesis SF.

Язык: Английский

Процитировано

3
Choroid plexus extracellular vesicle transport of blood-borne insulin-like growth factor 1 to the hippocampus of the immature brain DOI Creative Commons
Niklas Ortenlöf,

Suvi Vallius,

Helena Karlsson

и другие.

PNAS Nexus, Год журнала: 2024, Номер 3(12)

Опубликована: Ноя. 12, 2024

Reduced serum level of insulin-like growth factor 1 (IGF-1), a major regulator perinatal development, in extremely preterm infants has been shown to be associated with neurodevelopmental impairment. To clarify the mechanism IGF-1 transport at blood-cerebrospinal fluid (CSF) barrier immature brain, we combined studies vivo piglet and rabbit models an vitro transwell cell culture model neonatal primary murine choroid plexus epithelial (ChPE) cells. We identified IGF-1-positive intracellular vesicles ChPE cells provided data indicating directional from basolateral apical media extracellular (EVs). Exposure human on side increased secretion EVs media. Mass spectrometry analysis displayed similarities protein content between derived CSF-derived cell-derived EVs. Furthermore, exposure caused enrichment transmembrane p24 trafficking 2, proteins important for media-derived Moreover, intraventricular injections pups resulted uptake displaying penetration through ependymal lining deep into hippocampus. Finally, rat hippocampus neurons internalization hippocampal soma neurites. In summary, describe pathway blood-borne blood-CSF brain.

Язык: Английский

Процитировано

3
Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T DOI Creative Commons

Xiuli Yang,

Yuguo Li, Hanzhang Lu

и другие.

MethodsX, Год журнала: 2025, Номер 14, С. 103175 - 103175

Опубликована: Янв. 18, 2025

Brain oxygen metabolism indicates the rate of energy consumption and is a potential marker pathological changes. Positron emission tomography (PET) gold standard for measuring metabolic rates using radioactive tracers. However, its application in preclinical studies, particularly with rodent animals, constrained by need arterial input function measurements on-site cyclotron facilities tracer preparation. As an alternative, non-invasive, non-contrast MRI techniques, such as T2-relaxation-under-spin-tagging (TRUST) phase-contrast (PC) MRI, can be used evaluating brain vivo. This study outlines step-by-step method implementing TRUST PC mice at 11.7T scanner. The proposed yields quantitative global approximately 20 min, paving way broader applications future pathophysiological studies.•Non-invasive assessment mice.•Quantitative measurement min.

Язык: Английский

Процитировано

0
Vascular and Metabolic Responses to Elevated Circulating PDGF-BB in Mice: A Multiparametric MRI Study DOI Creative Commons

Xiuli Yang,

Jiekang Wang, Yuguo Li

и другие.

Опубликована: Фев. 11, 2025

Article Vascular and Metabolic Responses to Elevated Circulating PDGF-BB in Mice: A Multiparametric MRI Study Xiuli Yang 1,†, Jiekang Wang 2,3,†, Yuguo Li 1,4, Mei Wan 2,3,*, Zhiliang Wei 1,4,* 1 Russell H. Morgan Department of Radiology Radiological Science, Johns Hopkins University School Medicine, Baltimore, MD 21205, USA 2 Orthopaedic Surgery, 3 Biomedical Engineering, 4 F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, * Correspondence: [email protected] (M.W.); [email protected] (Z.W.) † These authors contributed equally this work. Received: 20 November 2024; Revised: December Accepted: 22 January 2025; Published: 11 February 2025 Abstract: circulating platelet-derived growth factor-BB (PDGF-BB) has been implicated the development various aged-related pathologies is recognized as a potential pro-aging factor. Although numerous studies have explored pathological roles PDGF-BB/PDGFRβ signaling pathway, few investigations dissected its function neurofunctional responses elevated PDGF-BB, primarily because in-vivo measurements are generally required assess neurofunction. To address knowledge gap, we characterized vascular metabolic vivo using multiparametric non-invasive non-contrast techniques conditional Pdgfb transgenic mouse model (PdgfbcTG) at 6 months age. Results indicated that PdgfbcTG mice exhibited decreased cerebral blood flow (p = 0.025), oxygen extraction 0.002), increased rate 0.035), mirroring changes observed human aging. The change was significantly higher (≥200.3%) compared naturally aged mice. This study provides evidence accelerates neurovascular

Язык: Английский

Процитировано

0
Vascular and Metabolic Responses to Elevated Circulating PDGF-BB in Mice: A Multiparametric MRI Study DOI

Xiuli Yang,

Jiekang Wang, Yuguo Li

и другие.

Опубликована: Апрель 2, 2025

Elevated circulating platelet-derived growth factor-BB (PDGF-BB) has been implicated in various aged-related pathologies and, therefore, is recognized as a potential pro-aging factor. Despite extensive studies on the pathological characterizations centering PDGF-BB/PDGFRβ signaling alterations, there limited research neurofunctional responses to elevated PDGF-BB, primarily because in-vivo measurements are generally required for studying neurofunction. To address this knowledge gap, we characterized vascular and metabolic PDGF-BB vivo using multiparametric non-invasive non-contrast MRI techniques conditional Pdgfb transgenic mouse model (PdgfbcTG) at 6 months of age. We found that PdgfbcTG mice exhibited decreased cerebral blood flow (P = 0.025), oxygen extraction 0.002), increased rate 0.035), which replicated changes observed human aging. Compared naturally aged mice, change was prominently higher (≥ 200.3%). Our study provides evidence accelerates neurovascular

Язык: Английский

Процитировано

0
Local wakefulness‐like activity of layer 5 cortex under general anaesthesia DOI
Jesús Pardo‐Valencia, Miryam Moreno‐Gomez,

Noelia Mercado

и другие.

The Journal of Physiology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 24, 2024

Abstract Consciousness, defined as being aware of and responsive to one's surroundings, is characteristic normal waking life typically lost during sleep general anaesthesia. The traditional view consciousness a global brain state has evolved toward more sophisticated interplay between local states, with the presence in awake wakefulness sleeping brain. However, this not clear for anaesthesia, where loss was recently suggested be associated brain‐wide synchrony that selectively involves layer 5 cortical pyramidal neurons across sensory, motor associative areas. According view, cortex should incompatible deep hypothesis deserves scrutinised causal manipulations. Here, we show unilateral chemogenetic activation sensorimotor isoflurane‐anaesthetised mice induces transition from slow‐wave activity tonic firing transfected hemisphere. This wakefulness‐like dramatically disrupts interhemispheric mirror‐image locations contralateral hemisphere, but does reduce level unconsciousness under nor transitions to/from Global may thus sufficient condition anaesthesia‐induced unconsciousness, necessary one, at least isoflurane Local can induced maintained encouraging further investigation into vs . aspects unconsciousness. image Key points neural correlates have states nuanced evident terms brains brains. concept remains unclear been involve neurons. We found chemogenetically anaesthetised without affecting depth anaesthesia or feature by compatible promoting vs.

Язык: Английский

Процитировано

1