Insects,
Год журнала:
2024,
Номер
15(12), С. 927 - 927
Опубликована: Ноя. 26, 2024
Acetamiprid
is
a
third-generation
neonicotinoid
insecticide
that
now
widely
employed
for
the
protection
of
crops
grown
in
outdoor
environments.
This
because
it
considerably
less
toxic
to
pollinating
insects
than
other
neonicotinoids.
Previous
studies
have
shown
acetamiprid
has
direct
physiological
effects
on
adult
and
larval
bees.
However,
its
potentially
healthy
gut
microbiota
honeybees
not
been
fully
elucidated.
To
further
investigate
effects,
worker
were
exposed
sucrose
solutions
containing
at
concentrations
0,
5,
25
mg/L
period
7
days
(adults)
4
(larvae).
The
results
showed
exposure
significantly
disrupted
honeybees’
intestinal
microbiota.
In
adults,
led
significant
increase
relative
abundance
Commensalibacter,
while
Bifidobacterium
Gilliamella
levels
decreased.
larvae,
we
observed
changes
microbial
composition,
notably
marked
reduction
Bombella.
Further
analysis
demonstrated
alterations
honeybee
larvae
associated
with
disturbances
metabolic
pathways
regulate
energy
metabolism
neurometabolism.
These
suggest
affects
bee
health
only
through
but
also
microbiota,
which
turn
affect
immune
function
study
underscores
need
evaluate
pesticides’
risks
from
microbiological
standpoint
offers
crucial
insights
into
how
impacts
by
modifying
support
more
comprehensive
assessment
similar
pesticides
regarding
health.
FEMS Microbiology Ecology,
Год журнала:
2024,
Номер
100(7)
Опубликована: Апрель 22, 2024
Abstract
The
Melipona
gut
microbiota
differs
from
other
social
bees,
being
characterized
by
the
absence
of
crucial
corbiculate
core
symbionts
and
a
high
occurrence
environmental
strains.
We
studied
microbial
diversity
composition
three
species
their
honey
to
understand
which
strains
are
obtained
horizontal
transmission
(HT)
pollination
environment,
represent
with
HT
hive/food
stores
or
(ST)
between
nestmates.
Bees
harbored
higher
alpha
different
more
species-specific
bacterial
than
honey.
fungal
communities
bee
samples
also
but
less
dissimilar.
As
expected,
eusocial
Snodgrassella
Gilliamella
were
absent
in
bees
that
had
prevalence
Lactobacillaceae
-
including
Lactobacillus
(formerly
known
as
Firm-5),
Bifidobacteriaceae,
Acetobacteraceae,
Streptococcaceae
mainly
close
Floricoccus,
putative
novel
symbiont
acquired
flowers.
They
might
have
co-evolved
these
via
ST,
along
Pectinatus
(Veillonellaceae)
HT,
Metschnikowia
Saccharomycetales
yeasts
plants/flowers,
possibly
compose
microbiota.
This
work
contributes
understanding
modes
transmission.
Environmental Toxicology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 22, 2024
ABSTRACT
Clothianidin
(CLO)
is
a
high‐frequently
detected
neonicotinoid
pesticide
in
fruits
and
vegetables,
whose
exposure
security
deserves
attention.
This
study
evaluated
the
apoptotic
toxicity
of
CLO
on
Caco‐2
cells
at
doses
100
nM,
10
μM,
1
mM.
After
exposure,
induced
to
remarkable
change
signaling
proteins
that
participated
process
cell
apoptosis,
including
caspase
3,
cleaved‐caspase
9.
treatment
further
decrease
mitochondrial
membrane
potential
increased
protein
level
cytochrome
C.
Reactive
oxygen
species
(ROS)
intracellular
Ca
2+
were
also
found
elevated,
indicating
an
oxidative
damage
caused
by
treatment.
Moreover,
production
ROS
occurred
advance
elevation,
since
inhibiting
could
recover
elevation
exposure.
The
metabolic
enzyme
P450
3A4
(CYP3A4)
was
downregulated
after
CLO.
Molecular
docking
simulation
indicated
had
good
binding
characteristics
with
CYP3A4.
Amino
acid
sites
Arg105,
Arg130,
Leu373
CYP3A4,
nitro
group
chlorothiazole
structure
might
be
action
target.
These
results
induce
effect
cells,
possibly
acting
through
combining
its
then
leading
stress
damage.
Thus,
risk
factor
for
human
intestinal
health.
Insects,
Год журнала:
2024,
Номер
15(12), С. 927 - 927
Опубликована: Ноя. 26, 2024
Acetamiprid
is
a
third-generation
neonicotinoid
insecticide
that
now
widely
employed
for
the
protection
of
crops
grown
in
outdoor
environments.
This
because
it
considerably
less
toxic
to
pollinating
insects
than
other
neonicotinoids.
Previous
studies
have
shown
acetamiprid
has
direct
physiological
effects
on
adult
and
larval
bees.
However,
its
potentially
healthy
gut
microbiota
honeybees
not
been
fully
elucidated.
To
further
investigate
effects,
worker
were
exposed
sucrose
solutions
containing
at
concentrations
0,
5,
25
mg/L
period
7
days
(adults)
4
(larvae).
The
results
showed
exposure
significantly
disrupted
honeybees’
intestinal
microbiota.
In
adults,
led
significant
increase
relative
abundance
Commensalibacter,
while
Bifidobacterium
Gilliamella
levels
decreased.
larvae,
we
observed
changes
microbial
composition,
notably
marked
reduction
Bombella.
Further
analysis
demonstrated
alterations
honeybee
larvae
associated
with
disturbances
metabolic
pathways
regulate
energy
metabolism
neurometabolism.
These
suggest
affects
bee
health
only
through
but
also
microbiota,
which
turn
affect
immune
function
study
underscores
need
evaluate
pesticides’
risks
from
microbiological
standpoint
offers
crucial
insights
into
how
impacts
by
modifying
support
more
comprehensive
assessment
similar
pesticides
regarding
health.
