8-[(4-benzylpiperazin-1-yl)methyl]-3-(2-chlorophenyl)-7-hydroxy-chromen-4-one is an activator of contractile activity of intestinal smooth muscles with reversible M2 cholinomimetic properties DOI Creative Commons
О. V. Tsymbalyuk, I. S. Voiteshenko, Sergiy A. Starosyla

и другие.

Studia Biologica, Год журнала: 2023, Номер 17(4), С. 15 - 36

Опубликована: Дек. 1, 2023

Background. Several pathologies (such as diabetes mellitus, Parkinson’s and Alzheimer’s diseases, multiple sclerosis, etc.) are accompanied by degeneration of cholinergic neurons, which key regulators the contractile function gastrointestinal tract walls, leading to atony paresis. An effective strategy for normalizing lack visceral SM is use drugs - selective agonists muscarinic acetylcholine receptors (mAChRs) M2 subtype. The high similarity structure agonist-binding sites different subtypes mAChRs causes problems develop ligands these receptors. Nowadays, there an urgent necessity subtype pharmacological tools laboratory research promising drugs. aim present was investigate effect 8-[(4-benzylpipe­razin-1-yl)methyl]-3-(2-chlorophenyl)-7-hydroxy-chromen-4-one (compound 1), in silico predicted bind mAChRs, on activity rat caecum circular smooth muscle. Materials Methods. carried out rats. studied tensometrically isometric mode preparations muscles Wistar kinetic properties individual spontaneous contractions were determined accordance with method multivariate mechanokinetic analysis calculation parameters phases contraction relaxation: time (τ0, τC τR), force (Fmax, FC FR), velocity (VC VR) impulse (Imax, IC IR). acetylcholine-induced analyzed calculating normalized maximum velocities (Vnc) relaxation (Vnr) phases. Results. It found that compound 1 caused increase amplitude contractions; this eliminated preincubation inhibitor AF-DX 116. revealed (0.1–50 μM) also has ability significantly activate functional colonic a dose-dependent manner, increasing frequency contractions, well their parameters. presence (0.1 solution washing muscle long leads significant tends reach stationary after 40 minutes its action. stable at least hour application caecum, reversible SM. Conclusions. Compound stimulates cecal exhibits properties.

Язык: Английский

Toll‐like receptor 4 deficiency ameliorates experimental ileitis and enteric neuropathy: Involvement of nitrergic and 5‐hydroxytryptaminergic neurotransmission DOI Creative Commons
Sofia Faggin, Silvia Cerantola, Valentina Caputi

и другие.

British Journal of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Abstract Background and Purpose Inflammatory bowel disease (IBD) patients display genetic polymorphisms in toll‐like receptor 4 (TLR4) genes, contributing to dysregulate enteric nervous system (ENS) circuits with increased levels of 5‐HT alteration the neuroimmune crosstalk. In this study, we investigated impact TLR4 signalling on mouse ENS dysfunction caused by dextran sulphate sodium (DSS)‐induced ileitis. Experimental Approach Male C57BL/6J (wild‐type [WT]) −/− mice (10 ± 2 weeks old) received 2% DSS drinking water for 5 days then were switched 3‐day regular water. Histological analysis proinflammatory cytokine mRNA assessed ileal samples. Gut motility was evaluated changes transit a fluorescent‐labelled marker isometric neuromuscular responses full‐thickness segments non‐receptor‐mediated stimuli. Alterations architecture confocal immunohistochemistry longitudinal muscle–myenteric plexus whole‐mount preparations. Key Results WT mice, treatment delayed gastrointestinal transit, myenteric neurodegeneration, reactive gliosis release cytokines. Enhanced cholinergic tachykinergic excitatory tone, inducible nitric oxide synthase (iNOS)‐mediated relaxation, 2A 3 receptor‐mediated observed during ileitis mice. deficiency reversed most functional morphological abnormalities. Conclusion Implications Our results demonstrate that activity influences severity ileitis, neuroglial plasticity, gut motility, nitrergic 5‐HTergic neurotransmissions. The interaction between our study appears be potential pharmacological target treat implicated IBD onset/progression.

Язык: Английский

Процитировано

0
Multichannel mapping of in vivo rat uterine myometrium exhibits both high and low frequency electrical activity in non-pregnancy DOI Creative Commons
Amy S. Garrett, Mathias W. Roesler, Omkar N. Athavale

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Март 27, 2024

The uterus exhibits intermittent electrophysiological activity in vivo. Although most active during labor, the non-pregnant can exhibit of comparable magnitude to early stages labor. In this study, two types flexible electrodes were utilized measure electrical uterine smooth muscle vivo anesthetized, rats. Flexible printed circuit placed on serosal surface horn six anesthetized Electrical was recorded for a duration 20-30 min. Activity contained components: high frequency (bursts) and an underlying low 'slow wave' which occurred concurrently. These components had dominant frequencies 6.82 ± 0.63 Hz burst 0.032 0.0055 slow wave frequency. There mean occurrence rate 0.76 0.23 bursts per minute 20.1 6.5 s. use multiple high-resolution enabled 2D mapping initiation propagation along horn. This approach has potential provide organ level detail help interpret non-invasive body recordings.

Язык: Английский

Процитировано

3
Implantable Bioelectronics for Real-time in vivo Recordings of Enteric Neural Activity DOI Creative Commons
Alexander J. Boys,

Amparo Güemes,

Rohit Gupta

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 27, 2024

Abstract The enteric nervous system represents a primary point of contact for host factors that influence bodily health and behavior. This division the autonomic is unique in both its extensivity, with neurons distributed throughout gastrointestinal tract from esophagus to rectum, capability local information processing. Here, we show construction validation bioelectronic device access neural produced processed tract. We designed an implant concurrent surgical procedure place recording within wall colon rodents. captured complex multi-frequency electrophysiological responses stimulants can record activity context mechanical mimicking gut motility. also feasibility utilizing this colonic freely-moving animals. work step forward devising functional devices understanding pathways gut-brain axis. One-Sentence Summary Bioelectronic real-time collection system.

Язык: Английский

Процитировано

2
TRPC5 channel participates in myocardial injury in chronic intermittent hypoxia DOI Creative Commons
Mengmeng Wang, Wen Wen, Yulan Chen

и другие.

Clinics, Год журнала: 2024, Номер 79, С. 100368 - 100368

Опубликована: Янв. 1, 2024

The purpose of this study is to develop an animal model Chronic Intermittent Hypoxia (CIH) and investigate the role TRPC5 channel in cardiac damage OSAHS rats. Twelve male Sprague Dawley rats were randomly divided into CIH group Normoxic Control (NC) group. Changes structure, function, pathology heart tissue observed through echocardiography, transmission electron microscopy, HE-staining, TUNEL staining. Interventricular Septum thickness at diastole (IVSd) End-Diastolic Volume (EDV) significantly increased, whereas LV ejection fraction shortening decreased. TEM showed that myofilaments loosely arranged, sarcomere length varied, cell matrix dissolved, mitochondrial cristae partly flocculent, outer membrane dissolved disappeared, some mitochondria swollen vacuolated. histopathological examination cardiomyocytes with granular degeneration, myocardial fibers broken disorganized, most nuclei vacuolar hypochromic. promoted oxidative stress, influx Ca2+, activation CaN/NFATc signaling pathway, which led pathological changes morphology ultrastructure cardiomyocytes, increase apoptosis, decrease contractility. These may be associated upregulation TRPC5.

Язык: Английский

Процитировано

2
Probiotics in Postoperative Pain Management DOI Open Access
Barbara Fyntanidou, Aikaterini Amaniti, Eleftheria Soulioti

и другие.

Journal of Personalized Medicine, Год журнала: 2023, Номер 13(12), С. 1645 - 1645

Опубликована: Ноя. 25, 2023

Postoperative pain is the unpleasant sensory and emotional experience after surgery, its origin being both inflammatory reaction induced by surgical trauma on abdominal wall splanchnic activation of nociceptors viscera, which are highly sensitive to distension, ischemia, inflammation. Nowadays, it well recognized that there a close relationship between gut microbiome perception, affected anesthesia manipulation. Thus, efforts restore disturbed via supplementation with beneficial bacteria, namely probiotics, seem be effective. In this article, knowledge gained mainly from experimental research topic analyzed, concluding message each probiotic strain works in own way towards relief.

Язык: Английский

Процитировано

5
Sterilization of free-ranging female capybaras (Hydrochoerus hydrochaeris): a comparison between two surgical techniques DOI Creative Commons
Fabiana Morse Gosson Jorge, Fernanda Battistella Passos Nunes, Pedro Nacib Jorge‐Neto

и другие.

Animal Reproduction, Год журнала: 2024, Номер 21(4)

Опубликована: Янв. 1, 2024

This study evaluated two surgical sterilization techniques in free-ranging female capybaras (

Язык: Английский

Процитировано

1
Network pharmacology combined with an animal model to reveal the material basis and mechanism of Amomum villosum in alleviating constipation in mice DOI

Shuangfeng Liu,

Yan Zhao, Sijin Li

и другие.

Gene, Год журнала: 2023, Номер 897, С. 148064 - 148064

Опубликована: Дек. 6, 2023

Язык: Английский

Процитировано

2
Short-Term Complications in Elective Joint Reconstruction: A Retrospective Assessment of the Impact of GLP-1 Agonists DOI Creative Commons
Richard A. Baum,

Alex La Poche,

Noshin Choudhury

и другие.

Journal of Orthopaedic Reports, Год журнала: 2024, Номер unknown, С. 100501 - 100501

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0
Preoperative Immunocyte-Derived Ratios Predict Postoperative Recovery of Gastrointestinal Motility after Colorectal Cancer Surgery DOI Open Access

Andreea Firut,

D Mărgăritescu,

Adina Turcu-Știolică

и другие.

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(19), С. 6338 - 6338

Опубликована: Окт. 3, 2023

The aim of this study was to assess the role immunocyte-derived ratios (IDRs), such as systemic immune-inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), and lymphocyte-to-monocyte (LMR), markers for postoperative recovery gastrointestinal function following colorectal cancer surgery. A retrospective analysis conducted on a consecutive cohort 260 patients who underwent radical surgery within timeframe spanning from January 2016 December 2022. Data concerning included I-FEED score, time pass flatus, toleration liquids in first 48 h, need nasogastric tube reinsertion immediate period. special emphasis allocated towards examination IDRs their interrelation with functional parameters. score exhibited positive correlation NLR, SII, PLR. univariate indicated that all IDRs, multiorgan resection, hemoglobin protein levels, regional nodal extent tumor (N), obesity significantly affected reinsertion. multivariate showed SII N1 stages were risk factors after resection only classifiers remained significant multivariable liquids. In summation, certain preoperative PLR, may hold potential predictive determinants

Язык: Английский

Процитировано

1
Multichannel mapping of in vivo rat uterine myometrium exhibits both high and low frequency electrical activity in non-pregnancy DOI Creative Commons
Amy S. Garrett, Mathias W. Roesler, Omkar N. Athavale

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Окт. 31, 2023

Abstract The uterus exhibits intermittent electrophysiological activity in vivo . Although most active during labor, the non-pregnant can exhibit of comparable magnitude to early stages labor. In this study, two types flexible electrodes were utilized measure electrical uterine smooth muscle anesthetized, rats. Flexible printed circuit placed on serosal surface horn six anesthetized Electrical was recorded for a duration 20–30 minutes. Activity contained components: high frequency (bursts) and an underlying low ‘slow wave’ which occurred concurrently. These components had dominant frequencies 6.82 ± 0.63 Hz burst 0.037 0.014 slow wave frequency. There mean occurrence rate 0.76 0.23 bursts per minute 20.1 6.5 s. use multiple high-resolution enabled 2D mapping initiation propagation along horn. This approach has potential provide organ level detail help interpret non-invasive body recordings.

Язык: Английский

Процитировано

0