In Silico Analysis of Limonoid-Based Antifeedants from Melia volkensii Targeting the Ryanodine Receptor in Spodoptera frugiperda DOI Creative Commons
Mohamed S. Rajab

Sciences of Phytochemistry, Год журнала: 2024, Номер 3(2), С. 98 - 104

Опубликована: Ноя. 9, 2024

Spodoptera frugiperda is an invasive pest causing significant crop losses worldwide. Resistance development and health environmental concerns associated with synthetic insecticides have prompted a search for eco-friendly biopesticides. Limonoids such as salannin, volkensin, volkensinone, isolated from the East African plant Melia volkensii, show antifeedant activity against S. larvae. Volkensin had ED50 of 3.5 µg/cm², volkensinone (a lactone volkensin) 6 salannin 13 µg/cm². Additional limonoids M. including salanninolide toosendanin, also displayed strong effects. With toosendanin already used commercially, re-evaluation volkensii compounds was conducted using in silico techniques. Docking simulations 3D models these ryanodine receptor protein revealed binding affinities -6.4 to -7.5 kcal/mol, comparable those chlorantraniliprole, commercial insecticide targeting receptors. These at two distinct sites align well in-vitro activity, underscoring volkensii’s potential environmentally friendly, receptor-targeted biopesticide frugiperda.

Язык: Английский

Endophytic establishment of native Bacillus thuringiensis strain in maize plants and its efficacy against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) DOI Creative Commons

J. Karshanal,

Vinay Kalia

Egyptian Journal of Biological Pest Control, Год журнала: 2023, Номер 33(1)

Опубликована: Авг. 16, 2023

Abstract Background Bacillus thuringiensis ( Bt ) is known as the most successful microbial insecticide worldwide used against lepidopteran insect pests in agriculture. Native isolate VKK5 showing insecticidal activity Spodoptera frugiperda (FAW) (J.E. Smith) (Lepidoptera: Noctuidae) was characterized B. (BtVKK5) on a morphological and molecular basis. Recent research has shown that can be established an endophytic organism for controlling pests. The present work aimed at assessing colonization of BtVKK5 endophyte five maize cultivars by seed treatment (ST), soil drenching (SD), foliar application (FA) combination all methods (ST + SD FA) its bioefficacy neonates FAW. Results Establishment inoculated endophytes cultivars, viz. Pusa HQPM7 Improved, Jawahar Hybrid Maize 1, Vivek 27 Improved (PVH27I), HQPM5 DMRH 1301, confirmed re-isolating from leaves plant ampicillin-selected agar plates. Estimation colony-forming units per gram leaf showed there significant difference strain among different inoculation methods. colonies were further substantiated amplification cry1A cry1E genes. Bioefficacy studies highest mortality (50%) ST FA method, followed (40%) PVH27I. Moreover, growth inhibition observed survived larvae plants vis-a-vis control. Conclusion plants, complemented with activity, could possibly lead to innovative approach management S. other borers.

Язык: Английский

Процитировано

3

Combination effect of azadirachtin and chlorantraniliprole with three synergists against a serious invasive agricultural pest Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI
Ranganathan Muthusamy,

Govindaraj Vengateswari,

Suresh Kumarasamy

и другие.

Biocatalysis and Agricultural Biotechnology, Год журнала: 2023, Номер 55, С. 102992 - 102992

Опубликована: Дек. 21, 2023

Язык: Английский

Процитировано

2

Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa DOI Creative Commons

Eric Tossou,

Ghislain T. Tepa-Yotto, Georg Goergen

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 15, 2024

Abstract The fall armyworm Spodoptera frugiperda (J.E. Smith) is a new serious destructive and widespread pest of corn which recently invaded subtropical regions worldwide. As this invasive species spreading across the continent, it vital to assess its susceptibility currently used insecticides establish potential underlying resistance mechanism better inform control programmes. In study, we characterized strains from eighteen populations different countries in West Africa, established their profiles main genotyped target site alleles. RFLP-PCR method showed that majority population tested was strain (72.5 – 100%) compared probable rice (0 27.5%). Tpi sequencing suspected revealed almost all samples analysed were (>97%). Additionally, among three tested, λ-cyhalothrin chlorpyrifos induced lowest toxicity against pest, while emamectin benzoate exhibited moderate toxicity. Synergism tests performed investigate biochemical by breakdown indicated metabolic enzymes (oxydases, esterase glutathion-S-transferase) play major roles observed western Africa. Target mutation (qPCR) combined with previous synergetic organophosphates pyrethroids could be due + amino acid mutations (presence F290V mutation) or (absence T929I mutation), respectively. results study provide valuable information for supporting decisions related sustainable applied management.

Язык: Английский

Процитировано

0

Loop-Mediated Isothermal Amplification for Detecting Gly-4891-Glu and Ile-4734 Multiple Mutations of Ryanodine Receptor in the Fall Armyworm, Spodoptera frugiperda DOI
Jing-Bo Lu,

Pei-Yun He,

Qi Luo

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(36), С. 19948 - 19956

Опубликована: Авг. 26, 2024

The key mutations, such as the Gly-4891-Glu substitution and Ile-4734 multiple substitutions within ryanodine receptors (RyR), are linked to diamide resistance in fall armyworm (FAW),

Язык: Английский

Процитировано

0

In Silico Analysis of Limonoid-Based Antifeedants from Melia volkensii Targeting the Ryanodine Receptor in Spodoptera frugiperda DOI Creative Commons
Mohamed S. Rajab

Sciences of Phytochemistry, Год журнала: 2024, Номер 3(2), С. 98 - 104

Опубликована: Ноя. 9, 2024

Spodoptera frugiperda is an invasive pest causing significant crop losses worldwide. Resistance development and health environmental concerns associated with synthetic insecticides have prompted a search for eco-friendly biopesticides. Limonoids such as salannin, volkensin, volkensinone, isolated from the East African plant Melia volkensii, show antifeedant activity against S. larvae. Volkensin had ED50 of 3.5 µg/cm², volkensinone (a lactone volkensin) 6 salannin 13 µg/cm². Additional limonoids M. including salanninolide toosendanin, also displayed strong effects. With toosendanin already used commercially, re-evaluation volkensii compounds was conducted using in silico techniques. Docking simulations 3D models these ryanodine receptor protein revealed binding affinities -6.4 to -7.5 kcal/mol, comparable those chlorantraniliprole, commercial insecticide targeting receptors. These at two distinct sites align well in-vitro activity, underscoring volkensii’s potential environmentally friendly, receptor-targeted biopesticide frugiperda.

Язык: Английский

Процитировано

0