Deuterium MRS for In Vivo Measurement of Lipogenesis in the Liver
NMR in Biomedicine,
Год журнала:
2025,
Номер
38(4)
Опубликована: Фев. 24, 2025
ABSTRACT
Hepatic
de
novo
lipogenesis
(DNL)
plays
a
key
role
in
the
pathogenesis
of
several
metabolic
diseases
that
affect
liver.
In
humans,
detection
deuterium
(
2
H)
triglycerides
from
very
low
density
lipoprotein
collected
blood
after
administration
deuterated
water
(D
O)
is
commonly
used
as
an
indirect
estimate
hepatic
DNL.
Here,
we
tested
rats
(1)
feasibility
to
detect
H‐labeling
directly
liver
lipids
vivo
by
using
noninvasive
H
MRS
and
(2)
what
extent
these
results
correlated
with
gold
standard
measurement
DNL
excised
tissue.
To
increase
DNL,
half
animals
n
=
4)
underwent
7‐week
dietary
intervention
which
fructose
was
provided
drinking
water.
Deuterium
data
were
acquired
single
voxel
placed
showed
combined
peak
methyl
methylene
resonances
1
week
administrati
NBM_70014
on
5%
D
O
calculated
NMR
extracted
The
lipid
level
measured
ex
estimates
r
0.81,
p
0.016).
These
demonstrate
direct
labeling
localized
indicate
potential
this
approach
measure
initial
observations
provide
basis
for
method
be
translated
develop
noninvasive,
quantitative
measurements
humans.
Язык: Английский
Evaluation of Hepatic Glucose and Palmitic Acid Metabolism in Rodents on High‐Fat Diet Using Deuterium Metabolic Imaging
Journal of Magnetic Resonance Imaging,
Год журнала:
2024,
Номер
unknown
Опубликована: Май 9, 2024
Background
One
of
the
main
features
several
metabolic
disorders
is
dysregulation
hepatic
glucose
and
lipid
metabolism.
Deuterium
imaging
(DMI)
allows
for
assessing
uptake
breakdown
2
H‐labeled
substrates,
giving
specific
insight
into
nutrient
processing
in
healthy
diseased
organs.
Thus,
DMI
could
be
a
useful
approach
analyzing
differences
liver
metabolism
subjects
to
gain
deeper
understanding
alterations
related
disorders.
Purpose
Evaluating
feasibility
as
tool
assessment
rodents
with
fatty
livers
(FLs).
Study
Type
Animal
Model.
Population
18
male
Sprague
Dawley
rats
on
standard
(SD,
n
=
9,
healthy)
high‐fat
diet
(HFD,
FL
disease).
Field
Strength/Sequence
Phase‐encoded
1D
pulse‐acquire
sequence
anatomy
co‐registered
phase‐encoded
3D
chemical
shift
H
at
9.4T.
Assessment
Localized
nonlocalized
spectroscopy
was
applied
eight
time
points
over
104
minutes
post
injection.
The
obtained
spectra
were
preprocessed
quantified
using
jMRUI
(v7.0)
resulting
amplitudes
translated
absolute
concentration
(mM)
according
natural
abundance
water
peak.
Statistical
Tests
Two‐way
repeated
measures
ANOVA
employed
assess
between‐group
differences,
statistical
significance
P
<
0.05.
Results
measurements
demonstrated
no
significant
difference
(
0.98)
[6,6′‐
]glucose
between
impaired
animals
(AUC
SD
1966.0
±
151.5
mM
‐
vs.
AUC
HFD
2027.0
167.6
mM·minutes).
In
group,
intrahepatic
palmitic
acid
d‐31
higher
57.4
17.0
mM·minutes,
33.3
10.5
mM·minutes),
but
without
owing
substantial
in‐group
variation
0.73).
Data
Conclusion
revealed
concentrations
disease
animals.
appears
evaluating
disease.
Level
Evidence
Technical
Efficacy
Stage
3.
Язык: Английский
Development of a tamarind-based functional beverage with partially-hydrolyzed agave syrup and the health effects of its consumption in C57BL/6 mice
Food Chemistry,
Год журнала:
2024,
Номер
447, С. 138935 - 138935
Опубликована: Март 4, 2024
Язык: Английский
Development and optimization of human deuterium MRSI at 3 T in the abdomen: feasibility in renal tumors following oral heavy water administration
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 8, 2024
Abstract
Purpose
To
establish
and
optimize
abdominal
deuterium
MRSI
in
conjunction
with
orally
administered
2
H-labelled
molecules.
Methods
A
flexible
transmit-receive
surface
coil
was
used
to
image
naturally
abundant
signal
phantoms
healthy
volunteers
after
H
O
a
patient
benign
renal
tumor
(oncocytoma).
Results
Water
lipid
peaks
were
fitted
high
confidence
from
both
unlocalized
spectra
voxels
within
the
liver,
kidney,
spleen
on
spectroscopic
imaging.
Artifacts
minimal
despite
concentration
stomach
immediately
ingestion,
which
can
be
problematic
use
of
volume
coil.
Conclusion
We
have
shown
feasibility
at
3
T
using
measurements
obtained
images
acquired
drinking
O.
The
limited
depth
penetration
may
advantages
characterizing
early
uptake
agents
organs
concentrations
pose
challenges
other
combinations.
Язык: Английский
Deuterium Metabolic Imaging Enables the Tracing of Substrate Fluxes Through the Tricarboxylic Acid Cycle in the Liver
NMR in Biomedicine,
Год журнала:
2024,
Номер
38(1)
Опубликована: Дек. 15, 2024
ABSTRACT
Alterations
in
tricarboxylic
acid
(TCA)
cycle
metabolism
are
associated
with
hepatic
metabolic
disorders.
Elevated
acetate
concentrations,
often
attributed
to
high
caloric
intake,
recognized
as
a
pivotal
factor
the
etiology
of
obesity
and
syndrome.
Therefore,
assessment
breakdown
TCA
activity
plays
central
role
understanding
impact
diet‐induced
alterations
on
liver
metabolism.
Magnetic
resonance‐based
deuterium
imaging
(DMI)
could
help
unravel
underlying
mechanisms
involved
disease
development
progression,
however,
application
conventional
deuterated
glucose
does
not
lead
substantial
enrichment
glutamine
glutamate.
This
study
aimed
demonstrate
feasibility
DMI
for
tracking
via
lean
fatty
(FL)
rats
using
3D
after
an
intraperitoneal
infusion
sodium
acetate‐d3
at
9.4T.
Localized
nonlocalized
spectra
acquired
10
time
points
post‐injection
over
130‐min
revealed
similar
intrahepatic
uptake
both
animal
groups
(AUC
FL
=
717.9
±
131.1
mM▯min
−1
,
AUC
605.1
119.9
p
0.62).
Metabolic
be
observed
emerging
glutamine/glutamate
(Glx)
peak
downstream
product
113.6
23.8
136.7
41.7
0.68).
showed
viability
substrate
flux
through
cycle,
underscoring
its
methodological
potential
processes
body.
Язык: Английский