Frontiers in Physiology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 20, 2024
Impaired
intestinal
integrity
in
broilers
reduces
performance
and
health,
highlighting
the
importance
of
accurately
measuring
permeability
(IP)
to
maintain
gut
health.
The
objective
this
study
was
evaluate
efficiency
iohexol
as
an
IP
marker
challenged
with
Eimeria
maxima,
Clostridium
perfringens,
or
both
during
peak
challenge
(day
[d]
21)
recovery
(d
28)
periods.
One-day-old
male
Ross
708
birds
(n
=
56)
were
distributed
into
4
treatment
groups:
NC
(no-challenge
control);
EM
(challenged
5,000
E.
maxima
sporulated
oocysts/bird
on
d
15);
CP
1.0
×
108
CFUs/bird
C.
perfringens
19
20);
+
by
co-infection
described).
On
21
28,
each
bird
received
dose
64.7
mg/kg
body
weight
via
oral
gavage.
One
hour
later,
blood
samples
collected
from
14
(12
EM)
per
group
7
(6
28.
For
lesion
scoring
ileum
collection,
sampled
Birds
groups
had
lower
gain
(BWG)
compared
19-21
(P
≤
0.05).
These
also
exhibited
significantly
greater
scores
markedly
higher
serum
levels
However,
no
significant
differences
observed
among
following
Moreover,
differentials
mRNA
abundance
key
tight
junction
proteins
(CLDN1,
CLDN2,
ZO3),
pro-inflammatory
cytokines
(IL-1β,
IFNγ,
IL-22),
health
markers
(GLP2,
OLFM4,
MUC2)
21.
In
conclusion,
demonstrates
that
is
effective
for
assessing
under
different
enteric
conditions.
Parasite Immunology,
Год журнала:
2024,
Номер
46(2)
Опубликована: Фев. 1, 2024
Abstract
Chicken
coccidiosis,
caused
by
Eimeria
protozoa,
affects
poultry
farming.
Toll‐like
receptors
(TLRs)
and
host
defence
peptides
(HDPs)
help
innate
immune
responses
to
eliminate
invading
pathogens,
but
their
roles
in
tenella
infection
remain
poorly
understood.
Herein,
14‐day‐old
chickens
were
treated
orally
with
50,000
E.
oocysts
the
cecum
was
dissected
at
different
timepoints.
mRNA
expression
of
10
chicken
TLRs
(chTLRs)
five
HDPs
measured
quantitative
real‐time
PCR.
chTLR7
chTLR15
upregulated
significantly
3
h
post‐infection
while
other
chTLRs
downregulated
(
p
<
.05).
chTLR1a,
chTLR1b,
chTLR2b
chTLR4
peaked
36
post‐infection,
chTLR3,
chTLR5
72
chTLR21
highest
among
chTLRs,
peaking
48
0.05).
For
HDPs,
cathelicidin
(CATH)
1
B1
liver‐expressed
antimicrobial
peptide
2
96
CATH
HDPs.
CATH2
CATH3
markedly
The
results
provide
insight
into
molecules
during
chicken,
indicate
that
may
mediate
resistance
coccidiosis.
Poultry Science,
Год журнала:
2024,
Номер
103(11), С. 104223 - 104223
Опубликована: Авг. 19, 2024
The
purpose
of
the
study
was
to
investigate
effects
reduced
dietary
calcium
(Ca)
and
phosphorus
(P)
level
supplementation
25-hydroxycholecalciferol
(25-OHD
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 10, 2024
Abstract
Background
This
study
investigated
effects
of
different
methionine
(Met)
supplementation
levels
in
a
reduced
protein
diet
on
growth
performance,
intestinal
health,
and
physiological
parameters
broilers
under
Eimeria
challenge.
A
total
600
fourteen-day-old
Cobb500
male
were
challenged
with
E.
maxima,
tenella,
acervulina
,
randomly
allocated
2
×
5
factorial
arrangement.
Birds
received
normal
diets
(20%
crude
protein,
NCP)
or
(17%
LCP),
containing
2.8,
4.4,
6.0,
7.6,
9.2
g/kg
Met.
Results
On
6
9
days
post
inoculation
(DPI),
increasing
Met
level
linearly
improved
the
performance
(
P
<
0.05).
Total
oocyst
shedding
increased
as
Duodenal
villus
height
(VH):crypt
depth
(CD)
LCP
groups
higher
DPI
0.01)
while
lower
0.05)
compared
to
NCP
groups.
Jejunal
CD
duodenal
VH:CD
changed
quadratically
DPI,
liver
glutathione
(GSH)
disulfide
(GSSG)
GSSG
increased,
whereas
GSH:GSSG
decreased
The
expression
amino
acid
transporters
zonula
occludens
claudin-1
expressions
cytokines
than
Interaction
found
for
IL-10
TNFα
0.05),
where
it
only
group
increased.
folate
metabolism
genes
these
Conclusion
These
results
revealed
regulatory
roles
including
oxidative
status,
nutrient
birds
fed
.
Poultry Science,
Год журнала:
2024,
Номер
103(12), С. 104483 - 104483
Опубликована: Ноя. 2, 2024
One
important
feature
of
corn
distillers
dried
grains
with
solubles
(DDGS)
is
its
high
leucine:lysine
ratio,
which
can
inhibit
chicken
growth
by
causing
branched-chain
amino
acid
(BCAA)
antagonism.
The
current
study
was
conducted
to
investigate
the
effects
BCAA
imbalance
inclusion
DDGS
and
whether
additional
dietary
valine
isoleucine
could
alleviate
negative
in
broilers.
A
total
640
0-d-old
male
Cobb
500
broilers
were
allocated
into
4
treatments
8
replicates
reared
until
d
42.
four
different
groups
as
follows:
1)
control
(CON)
group
(corn-soybean
meal-based
diet);
2)
30%
(30D)
(replacing
soybean
meal
DDGS);
3)
30D
+
(30DB)
group;
4)
30DB
provide
same
leucine:valine
leucine:isoleucine
ratios
CON
(30DBB).
analyzed
1.36/1.41/1.46
(starter/grower/finisher
phase),
whereas
average
1.61/1.70/1.78
phase).
(30D,
30DB,
30DBB)
negatively
affected
body
weight
(BW)
from
7
42
BW
gain
(BWG),
feed
intake,
carcass
weight,
breast
muscle
jejunal
ileal
villus
height:crypt
depth
during
overall
period
(d
0
42)
(P
<
0.05).
Furthermore,
significantly
altered
expression
levels
tight
junction
proteins,
mechanistic
target
rapamycin
(mTOR)
pathway-related
genes,
catabolism
AA
transporters
compared
0.01).
showed
differences
beta-diversity
indices
30DBB
showing
lowest
21
BWG
had
largest
most
measurements.
In
conclusion,
excessive
replacement
increase
leucine
levels,
may
affect
growth.
Additionally,
inappropriate
further
decrease
performance.
Frontiers in Physiology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 20, 2024
Impaired
intestinal
integrity
in
broilers
reduces
performance
and
health,
highlighting
the
importance
of
accurately
measuring
permeability
(IP)
to
maintain
gut
health.
The
objective
this
study
was
evaluate
efficiency
iohexol
as
an
IP
marker
challenged
with
Eimeria
maxima,
Clostridium
perfringens,
or
both
during
peak
challenge
(day
[d]
21)
recovery
(d
28)
periods.
One-day-old
male
Ross
708
birds
(n
=
56)
were
distributed
into
4
treatment
groups:
NC
(no-challenge
control);
EM
(challenged
5,000
E.
maxima
sporulated
oocysts/bird
on
d
15);
CP
1.0
×
108
CFUs/bird
C.
perfringens
19
20);
+
by
co-infection
described).
On
21
28,
each
bird
received
dose
64.7
mg/kg
body
weight
via
oral
gavage.
One
hour
later,
blood
samples
collected
from
14
(12
EM)
per
group
7
(6
28.
For
lesion
scoring
ileum
collection,
sampled
Birds
groups
had
lower
gain
(BWG)
compared
19-21
(P
≤
0.05).
These
also
exhibited
significantly
greater
scores
markedly
higher
serum
levels
However,
no
significant
differences
observed
among
following
Moreover,
differentials
mRNA
abundance
key
tight
junction
proteins
(CLDN1,
CLDN2,
ZO3),
pro-inflammatory
cytokines
(IL-1β,
IFNγ,
IL-22),
health
markers
(GLP2,
OLFM4,
MUC2)
21.
In
conclusion,
demonstrates
that
is
effective
for
assessing
under
different
enteric
conditions.
