Epitranscriptomic regulation of HIF-1: bidirectional regulatory pathways
Molecular Medicine,
Год журнала:
2025,
Номер
31(1)
Опубликована: Март 18, 2025
Abstract
Background
Epitranscriptomics,
the
study
of
RNA
modifications
such
as
N
6
-methyladenosine
(m
A),
provides
a
novel
layer
gene
expression
regulation
with
implications
for
numerous
biological
processes,
including
cellular
adaptation
to
hypoxia.
Hypoxia-inducible
factor-1
(HIF-1),
master
regulator
response
low
oxygen,
plays
critical
role
in
adaptive
and
pathological
cancer,
ischemic
heart
disease,
metabolic
disorders.
Recent
discoveries
accent
dynamic
interplay
between
m
A
HIF-1
signaling,
revealing
complex
bidirectional
regulatory
network.
While
roles
other
remain
largely
unexplored,
emerging
evidence
suggests
their
potential
significance.
Main
body
This
review
examines
reciprocal
epitranscriptomic
machinery,
writers,
readers,
erasers.
modulates
key
components,
while
its
own
mRNA
is
regulated
by
modifications,
positioning
both
target
this
system.
interaction
enhances
our
understanding
hypoxic
responses
opens
avenues
clinical
applications
treating
conditions
like
cancer
disease.
Promising
progress
has
been
made
developing
selective
inhibitors
targeting
A-HIF-1
axis.
However,
challenges
off-target
effects
complexity
modification
dynamics
significant
barriers
translation.
Conclusion
The
intricate
highlights
epitranscriptomics
hypoxia-driven
processes.
Further
research
into
these
networks
could
drive
therapeutic
innovation
hypoxia-related
conditions.
Overcoming
specificity
will
be
essential
realizing
therapies.
Язык: Английский
Quantitative proteomics identifies plasma protein alterations that associate with metabolic and thrombotic profile changes after bariatric surgery
Diabetes Obesity and Metabolism,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
Abstract
Objective
Roux‐en‐Y
gastric
bypass
(RYGB)
surgery
has
been
shown
to
lead
favourable
health
outcomes
in
obese
patients.
However,
the
molecular
changes
that
occur
and
how
they
relate
clinical
measures
are
poorly
understood.
Here,
we
characterise
proteomic
alterations
plasma
of
RYGB
patients
before
9
months
after
using
quantitative
proteomics.
Methods
Plasma
proteomics
was
performed
by
sequential
window
acquisition
all
theoretical
fragment
ion
spectra
mass
spectrometry
(SWATH‐MS)
identify
quantify
differentially
abundant
proteins.
Relationships
between
significantly
altered
proteins
markers
were
examined.
A
gene
set
enrichment
analysis
also
conducted
pathways.
Results
From
analysis,
27
increased,
43
decreased
abundance
surgery,
providing
insights
into
physiological
accompany
weight
loss.
Proteins
including
sex
hormone
binding
globulin
(SHBG),
inter‐alpha‐trypsin
inhibitor
heavy
chain
3
(ITIH3)
apolipoprotein
D
(APOD),
which
increased
post‐surgery,
highlight
improvements
lipid
regulation,
insulin
sensitivity
inflammation.
involved
coagulation,
α2‐macroglobulin,
kallikrein‐B1,
prothrombin,
factor
(FX,
FXI
FXII),
exhibited
reduced
levels,
aligning
with
a
decrease
thrombotic
potential.
Conclusions
These
findings
provide
mechanistic
understanding
bariatric
leads
systemic
metabolic
haemostatic
pathways,
thus
favourably
modulating
risk
developing
cardiovascular
disease.
Язык: Английский