From injury to repair: the therapeutic potential of induced pluripotent stem cells in heart failure
Regenerative medicine reports .,
Год журнала:
2025,
Номер
2(1), С. 22 - 30
Опубликована: Март 1, 2025
Heart
failure
is
a
complex
clinical
syndrome
characterized
by
the
heart’s
inability
to
pump
sufficient
blood
meet
body’s
metabolic
demands,
resulting
in
symptoms
such
as
dyspnea,
fatigue,
and
fluid
retention.
Despite
significant
advances
pharmacological
treatments
device
therapies,
prognosis
for
patients
with
advanced
heart
remains
poor.
This
underscores
urgent
need
innovative
regenerative
therapies
aimed
at
restoring
cardiac
function
improving
quality
of
life
suffering
from
this
debilitating
condition.
article
provides
comprehensive
overview
pathophysiologic
mechanisms
underlying
end-stage
failure,
which
include
myocardial
systolic
diastolic
dysfunction,
neuroendocrine
activation,
inflammation,
oxidative
stress.
Collectively,
these
lead
progressive
deterioration
structure
function,
culminating
failure.
The
also
examines
differences
between
ischemic
non-ischemic
cardiomyopathies
their
implications
treatment
strategies
prognosis.
Furthermore,
review
explores
application
medicine
focusing
on
its
potential
repair
functional
recovery.
In
field
medicine,
induced
pluripotent
stem
cells
derived
cardiomyocytes
are
considered
cutting-edge
technologies
repair.
Induced
can
differentiate
into
cardiomyocytes,
thereby
offering
new
hope
regeneration.
However,
current
research
faces
several
challenges,
including
maturity
integration
immunogenicity
concerns,
difficulties
scaling
up
production
applications.
Additionally,
ethical
issues
related
cell
technology,
sourcing
tumorigenic
risks,
must
be
carefully
addressed.
highlights
technology
treating
while
emphasizing
complexities
involved
transitioning
laboratory
practice.
Язык: Английский
Biomimetic Approaches in the Development of Optimised 3D Culture Environments for Drug Discovery in Cardiac Disease
Biomimetics,
Год журнала:
2025,
Номер
10(4), С. 204 - 204
Опубликована: Март 26, 2025
Cardiovascular
disease
remains
the
leading
cause
of
death
worldwide,
yet
despite
massive
investment
in
drug
discovery,
progress
cardiovascular
drugs
from
lab
to
clinic
slow.
It
is
a
complex,
costly
pathway
discovery
and
failure
becomes
more
expensive
as
progresses
along
this
pathway.
The
focus
has
begun
shift
optimisation
vitro
culture
methodologies,
not
only
because
these
must
be
undertaken
are
earlier
on
pathway,
but
also
principles
3Rs
have
become
embedded
national
international
legislation
regulation.
Numerous
studies
shown
myocyte
cell
behaviour
much
physiologically
relevant
3D
compared
2D
culture,
highlighting
advantages
using
3D-based
models,
whether
microfluidic
or
otherwise,
for
preclinical
screening.
This
review
aims
provide
an
overview
challenges
limitations
traditional
routes,
successes
field
models
discovery.
focuses
particular
role
biomimicry
can
play,
around
implementation
within
commercial
Язык: Английский
Synchronous beating between xenografted human cardiomyocytes and host zebrafish embryonic hearts
Biochemical and Biophysical Research Communications,
Год журнала:
2025,
Номер
769, С. 151933 - 151933
Опубликована: Май 2, 2025
Injured
human
hearts
are
fibrotic,
whereas
zebrafish
functionally
regenerate
following
myocardial
injury.
The
unique
regeneration
niche
microenvironment
has
been
extensively
studied
in
hearts.
However
whether
this
can
be
extrapolated
to
humans
remains
unclear
owing
significant
species
differences.
We
xenografted
induced
pluripotent
stem
cell-derived
cardiomyocytes
(hiCMs)
into
the
cardiac
region
of
one-day
post-fertilized
embryos
and
established
a
xenograft
model
hiCMs.
This
used
explore
behavior
hiCMs
transplanted
Fluctuations
fluorescence
intensity
genetically
encoded
calcium
indicator
protein
GCaMP
indicated
that
donor
were
beating.
analyzed
synchronization
+
heart.
found
synchronous
beating
between
host
40
%
with
GCaMP-hiPSCs.
Our
chimeric
heart
potential
bridge
capacity
gap
proming
future
applications.
Язык: Английский
ω-6 PUFA-enriched membrane phospholipid composition of cardiomyocytes increases the susceptibility to iron-induced ferroptosis and inflammation
APOPTOSIS,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 17, 2025
Язык: Английский
The role of iPSC research for insight in inherited arrhythmia conditions
Heart Rhythm,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
Язык: Английский
An hiPSC-CM approach for electrophysiological phenotyping of a patient-specific case of short-coupled TdP
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 18, 2024
A
healthy
young
woman,
age
26
without
prior
cardiac
complications,
experienced
an
out-of-hospital
arrest
caused
by
ventricular
fibrillation
(VF),
which
coincided
with
a
fever.
Comprehensive
diagnostics
including
echo,
CMR,
exercise
testing,
and
genetic
sequencing,
did
not
identify
any
potential
cause.
This
led
to
the
diagnosis
of
idiopathic
VF
installment
implantable
cardioverter
defibrillator,
six
months
later
appropriately
intervened
another
episode
under
conditions
comparable
first
event.
second
diagnostic
opinion
concluded
short-coupled
Torsade
de
Pointes
(scTdP),
patient
was
started
on
verapamil
treatment.
From
this
patient,
human
induced
pluripotent
stem
cell
cardiomyocyte
(hiPSC-CM)
lines
were
generated
study
cellular
electrophysiology.
Without
known
pathogenic
variation,
no
isogenic
control
line
could
be
produced,
therefore
age-
sex-matched
hiPSC-CM
used.
Cellular
electrophysiology
studied
in
these
cardiomyocytes
using
calcium-
voltage
sensitive
fluorescent
dyes
measurements
carried
out
at
37
°C
39
°C,
mimic
condition
hyperthermia
patient.
mRNA
expression
electrophysiologically
relevant
genes
analyzed
underlying
mechanism.
Calcium
transients
measured
physiological
temperature
indicated
occurrence
early
after
(EATs).
Strikingly,
incidence
EATs
further
increased.
Membrane
data
from
also
revealed
shorter
action
potentials
that,
combined
EATs,
indicate
premature
release
calcium
during
diastole,
responsible
for
extrasystoles
Gene
profiles
mainly
downregulated
but
clearly
aid
unraveling
mechanism
behind
EATs.
Pharmacological
screening
performed
evaluate
treatment
regimen
determine
While
verapamil,
dantrolene,
flecainide
decrease
handling
parameters
affected
indicating
functionality
drugs.
patient-specific
case
electrophysiological
phenotyping
resulted
hypothesis
possible
scTdP
arrhythmias,
accentuates
applicability
disease
modeling
phenotyping.
Язык: Английский
Pioneering Use of Extracorporeal Life Support in Intraoperative Cardiac Arrest in Dubai: A Case Report
Strahil Kotsev,
Nadine B Nour,
Muhammad Sharif Allah Dad
и другие.
Cureus,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 28, 2024
We
describe,
to
our
knowledge,
the
first
use
in
Dubai
of
extracorporeal
life
support
(ECLS)
a
patient
who
suffered
intraoperative
cardiac
arrest
due
presumed
channelopathy.
A
40-year-old
presented
for
open
myomectomy
surgery.
She
had
no
other
medical
problems
apart
from
obesity.
The
denied
any
family
history
surgery
or
anesthesia-related
complications.
Her
initial
electrocardiogram
(ECG)
and
laboratory
results
were
within
reference
limits.
Intraoperatively,
sudden
arrest,
which
she
was
resuscitated.
Point-of-care
ultrasound
intensive
care
unit
(ICU)-performed
echocardiography
revealed
severely
reduced
left
ventricular
contractility.
Veno-arterial
membrane
oxygenation
(V-A
ECMO)
intra-aortic
balloon
pump
utilized
immediate
postoperative
period.
Although
patient's
heart
sustained
more
than
300
DC
shocks,
it
recovered.
Family
members
that
brother
sister
died
their
20s
death.
Another
been
resuscitated
few
years
ago
too.
case
illustrates
importance
disclosure
relevant
information.
It
supports
notion
ECLS
can
be
used
period
if
surgical
hemostasis
is
achieved.
Controversies,
such
as
heparinization
risk
bleeding,
transthoracic
(TTE)
versus
transesophageal
(TEE),
timely
insertion
intracardiac
defibrillator,
genetic
screening,
are
discussed.
learning
point
clinicians
do
not
work
vacuum.
Organizational
leadership
greatly
impact
outcomes,
creating
conditions
safer
care.
Язык: Английский