Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

Journal of Integrative Plant Biology, Год журнала: 2022, Номер 64(6), С. 1281 - 1294

Опубликована: Март 6, 2022

Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, technique that significantly accelerates the crop breeding trajectory. Traditionally, vitro culture generate DHs, but this limited by species and genotype recalcitrance. In vivo induction (HI) through seed widely efficiently maize was recently extended several other crops. Here we show HI can be triggered mutation of DMP maternal inducer genes allopolyploid (allotetraploid) Brassica napus Nicotiana tabacum. We developed pipeline for selection orthologs clustered regularly interspaced palindromic repeats mutagenesis demonstrated average amphihaploid rates 2.4% 1.2% multiple B. N. tabacum genotypes, respectively. These results further confirmed ability gene polyploid dicot The DMP-HI system offers novel DH facilitate these success approach conservation dicots suggest broad applicability

Язык: Английский

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Embryo Rescue in Plant Breeding

Plants, Год журнала: 2023, Номер 12(17), С. 3106 - 3106

Опубликована: Авг. 29, 2023

Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote development an immature or lethal embryo into viable plant. Intraspecific, interspecific, intergeneric crosses allow introgression important alleles agricultural interest from wild species, such as resistance tolerance abiotic biotic stresses morphological traits crops. However, pre-zygotic post-zygotic reproductive barriers often present challenges achieving hybridization. Pre-zygotic manifest incompatibility reactions hinder pollen germination, tube growth, penetration ovule occurring various tissues, stigma, style, ovary. To overcome these barriers, several strategies employed, including cut-style graft-on-style techniques, utilization mixed distinct placenta pollination, pollination. On other hand, act at different tissues stages ranging early subsequent growth reproduction offspring. Many genera result abortion due failure endosperm development. In cases, needed hybrids. holds great promise for not only facilitating but also obtaining haploids, doubled manipulating ploidy levels chromosome engineering by monosomic disomic addition well substitution lines. Furthermore, can be shorten cycle propagation rare plants. Additionally, it has been repeatedly study embryonic development, especially embryo-lethal mutants. The widely procedure is embryos taken placed directly on media. certain ovule, ovaries placentas enables young zygote stage maturity.

Язык: Английский

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Apomixis and the paradox of sex in plants

Annals of Botany, Год журнала: 2024, Номер 134(1), С. 1 - 18

Опубликована: Март 18, 2024

Abstract Background The predominance of sex in eukaryotes, despite the high costs meiosis and mating, remains an evolutionary enigma. Many theories have been proposed, none them being conclusive on its own, they are, part, not well applicable to land plants. Sexual reproduction is obligate embryophytes for great majority species. Scope This review compares main forms sexual asexual ferns angiosperms, based generation cycling sporophyte gametophyte (leaving vegetative propagation aside). benefits maintenance genomic integrity comparison asexuality are discussed light developmental, evolutionary, genetic phylogenetic studies. Conclusions Asexual represents modifications pathway, with various facultative sexuality. For plants, provides direct DNA repair mechanisms oxidative damage reproductive tissues. ploidy alternations meiosis–syngamy cycles prolonged multicellular stages haploid phase gametophytes provide a efficiency purifying selection against recessive deleterious mutations. lineages might buffer effects such mutations via polyploidy can purge mutational load role organelle–nuclear genome compatibility understood. In plants general, mating low because predominant hermaphroditism. Phylogenetic patterns archaeplastid clade suggest that frequencies sexuality concomitant stepwise increase intrinsic extrinsic stress factors. Furthermore, expansion size would potential load. appears be essential keeping long-term integrity, only rare combinations factors allow shifts asexuality.

Язык: Английский

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Androgenesis in soybean (Glycine max (L.) Merr.): a critical revisit

In Vitro Cellular & Developmental Biology - Plant, Год журнала: 2024, Номер 60(1), С. 1 - 15

Опубликована: Янв. 4, 2024

Язык: Английский

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Establishment and maintenance of embryogenic cell fate during microspore embryogenesis

The Plant Journal, Год журнала: 2025, Номер 121(4)

Опубликована: Фев. 1, 2025

SUMMARY Microspore embryogenesis is a type of in vitro totipotency which the immature male gametophyte (pollen) develops into haploid embryo after an abiotic stress treatment. In Brassica napus , heat‐stress treatment gametophytes induces development different types multicellular embryogenic structures, each with cellular characteristics and capacity to form differentiated embryo. The origin early these structures have not been determined. We used two‐photon excitation fluorescence microscopy time‐lapse imaging cells expressing either LEAFY COTYLEDON1 ( LEC1 ) identity reporter or DR5v2 auxin response follow starting at single‐ few‐cell stage. show for first time that developmental fate defined by symmetry division plane also predicts timing subsequent pollen wall (exine) rupture: suspensorless embryos develop symmetric undergo late exine rupture, while suspensor‐bearing callus asymmetric rupture. Live captured previously unknown dynamic expression patterns are associated changes integrity. This study highlights plasticity cultured uncovers new roles cell defining maintaining during microspore embryogenesis.

Язык: Английский

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The Roads to Haploid Embryogenesis

Plants, Год журнала: 2023, Номер 12(2), С. 243 - 243

Опубликована: Янв. 5, 2023

Although zygotic embryogenesis is usually studied in the field of seed biology, great attention has been paid to methods used generate haploid embryos due their applications crop breeding. These mainly include two for embryogenesis: vitro microspore and vivo embryogenesis. culture systems maize induction were discovered 1960s, little known about molecular mechanisms underlying formation. In recent years, major breakthroughs have made systems, several key factors, such as matrilineal (MTL), baby boom (BBM), domain unknown function 679 membrane protein (DMP), egg cell-specific (ECS) that trigger embryo production both crops Arabidopsis models identified. The discovery these inducers indicates highly related gamete development, fertilization, genome stability ealry embryos. Here, based on efforts identify players understand its mechanisms, we summarize different paths embryogenesis, discuss generation potential haploid-inducing factors could assist cells bypassing fertilization initiate or elimination zygotes after form embryos, central endosperms a prerequisite step Deciphering cellular increasing efficiency, establishing other are critical promoting application technology breeding, should be addressed further studies.

Язык: Английский

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Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Plants, Год журнала: 2023, Номер 12(9), С. 1774 - 1774

Опубликована: Апрель 26, 2023

Anther culture is an efficient biotechnological tool in modern plant breeding programs to produce new varieties and parental lines hybrid seed productions. However, some bottlenecks-low induction rate, genotype dependency, albinism-restrict the widespread utilization of vitro anther rice breeding, especially Oryza sativa ssp. indica (indica) genotypes, while improved protocol can shorten process breeding. Three different media (N6NDK, N6NDZ, Ali-1) four regeneration (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested with five genotypes increase efficiency androgenesis (number calli regenerated green plantlets). The production was more on N6NDK medium average 88.26 calli/100 anthers N6NDZ 103.88 as compared Ali-1 6.96 anthers. plantlets greater when produced (2.15 plantlets/100 anthers) (1.18 anthers). Highest (4.7 achieved mMSNBK1 used utilizing medium. In best overall treatment (N6NDK medium), plantlets. influenced efficiency, ranged from 0.4 8.4 ploidy level 106 acclimatized characterized flow cytometric analyses calculate percentage spontaneous chromosome doubling. Altogether, 48 haploid-, 55 diploid-, 2 tetraploid- 1 mixoploid identified among regenerant plantlets, doubling 51.89%. Utilization DH plants have been integrated a routine method Hungarian program. tetraploid be explored for their potential offer scopes research directions future.

Язык: Английский

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Haploid identification in maize

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 19, 2024

Doubled haploid (DH) line production through in vivo maternal induction is widely adopted maize breeding programs. The established protocol for DH includes four steps namely induction, identification, genome doubling of haploid, and self-fertilization doubled haploids. Since modern inducers still produce relatively small portion haploids among undesirable hybrid kernels, identification typically laborious, costly, time-consuming, making this step the second foremost technique. This manuscript reviews numerous methods from different approaches including innate differences diploids, biomarkers integrated inducers, automated seed sorting. phenotypic differentiation, genetic basis, advantages, limitations each biomarker system are highlighted. Several sorting research groups also discussed regarding platform or instrument used, time, accuracy, limitations, challenges before they go commercialization. past selection was focusing on finding distinguishable marker systems with key to effectiveness. current adopting multiple reliable efficiency while seeking possibility automation. Fully high-throughput would be promising near future robustness retaining feasible level accuracy. that can meet between three major constraints (time, workforce, budget) scale best option.

Язык: Английский

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Androgenesis in indica rice: A comparative competency in development of doubled haploids

PLoS ONE, Год журнала: 2022, Номер 17(5), С. e0267442 - e0267442

Опубликована: Май 5, 2022

Rice is critical to global food security which demands immediate attention meet the ever-growing population. Development of improved variety major focus area research, in doubled haploid (DH) technology plays a vital role. Since, androgenesis shows its potential DH production, this method was not capitalized specially indica rice due recalcitrant nature tissue culture. Success governed by many important factors such as stage anther, pre-treatment conditions, accurate concentrations media, and plant growth regulators. Though reports are abundant rice, most them either used japonica or specific cultivar ecotypes. In study, media combination established successful producing haploids from F 1 s Savitri x Pokkali, IR20 Mahulata along with popular hybrids Arize 8433DT, 6453, Bold, Swift Gold. Out 12 different combinations tested, 5 durations cold-treatments studied, N6 2,4-D (2.0 mg/l) BAP (0.5 7 th day cold found be effective all for callus induction. Among s, hybrid, 8433DT showed highest 52% case green shoot regeneration, MS NAA mg/l), Kn (1.0 (MS+C4) efficient six treatments studied 58.25% regeneration 8433DT. Further, MS+C4 proline (5.0 increased rate 85.99%. Besides, (0.1 50 g/l sucrose supporting root induction F1s. This study claims establishment genotype independent androgenic protocol could improvement.

Язык: Английский

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Genetic Augmentation of Legume Crops Using Genomic Resources and Genotyping Platforms for Nutritional Food Security

Plants, Год журнала: 2022, Номер 11(14), С. 1866 - 1866

Опубликована: Июль 18, 2022

Recent advances in next generation sequencing (NGS) technologies have led the surge of genomic resources for improvement legume crops. Advances high throughput genotyping (HTG) and phenotyping (HTP) enable breeders to improve crops more precisely efficiently. Now, breeder can reshuffle natural gene combinations their choice enhance genetic potential These are efficiently deployed through molecular breeding approaches augmentation important crops, such as chickpea, cowpea, pigeonpea, groundnut, common bean, lentil, pea, well other underutilized In future, NGS, HTG, HTP will help identification assembly superior haplotypes tailor crop varieties haplotype-based breeding. This review article focuses on recent development resource databases deployment programmes secure global food security.

Язык: Английский

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