MYB transcription factors—master regulators of phenylpropanoid biosynthesis and diverse developmental and stress responses

Plant Cell Reports, Год журнала: 2022, Номер 41(12), С. 2245 - 2260

Опубликована: Сен. 29, 2022

Язык: Английский

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Anthocyanin Biosynthesis Induced by MYB Transcription Factors in Plants

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11701 - 11701

Опубликована: Окт. 2, 2022

Anthocyanins act as polyphenolic pigment that is ubiquitously found in plants. play a role not only health-promoting an antioxidant, but also protection against all kinds of abiotic and biotic stresses. Most recent studies have MYB transcription factors (MYB TFs) could positively or negatively regulate anthocyanin biosynthesis. Understanding the roles TFs essential elucidating how accumulation anthocyanin. In review, we summarized signaling pathways medicated by during biosynthesis including jasmonic acid (JA) pathway, cytokinins (CKs) temperature-induced, light signal, 26S proteasome NAC TFs, bHLH TFs. Moreover, structural regulator genes induced target bound activated suppressed crosstalk between other proteins, were to be vitally important regulation this study, focus on knowledge concerning mechanism biosynthesis, covering expression, protein expression.

Язык: Английский

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Diverse roles of MYB transcription factors in plants

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

MYB transcription factors (TFs), one of the largest TF families in plants, are involved various plant-specific processes as central regulators, such phenylpropanoid metabolism, cell cycle, formation root hair and trichome, phytohormones responses, reproductive growth abiotic or biotic stress responses. Here we summarized multiple roles explained molecular mechanisms TFs plant development adaptation. The exploration contributes to a better comprehension regulation environmental adaptability.

Язык: Английский

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The coordinated interaction or regulation between floral pigments and volatile organic compounds

Horticultural Plant Journal, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Floral color and scent are crucial for plant-environment interactions, especially in reproduction by attracting pollinators fertilization. They also have wide applications cosmetic, pharmaceutical, nutraceutical industries. Plant pigments typically classified as chlorophylls, flavonoids, carotenoids, betalains, while volatile organic compounds (VOCs) grouped terpenes, phenylpropanoids/benzenoids, fatty acid derivatives. Significant progresses been made understanding the biosynthesis regulation of these floral VOCs. Despite differences their biosynthetic pathways, VOCs biochemically connected exhibit synergistic interactions during flower development response to biotic abiotic stresses, suggesting existence pleiotropic regulators or complex mechanisms co-regulating biosynthesis. In this review, we summarize outline metabolite pathways mainly integrating phenylpropanoids/benzenoids. We provide a series scenarios illustrating coordinated scent. Finally, suggest areas future research. hope review will spark interest research direction stimulate further studies.

Язык: Английский

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Unveiling the power of MYB transcription factors: Master regulators of multi-stress responses and development in cotton

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 276, С. 133885 - 133885

Опубликована: Июль 16, 2024

Язык: Английский

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Ethylene positively regulates anthocyanin synthesis in 'Viviana' lily via the LvMYB5-LvERF113-LvMYB1 module

Horticulture Research, Год журнала: 2025, Номер 12(6)

Опубликована: Фев. 25, 2025

Abstract Ethylene (ET) influences the synthesis of anthocyanins, although its regulatory effects can differ significantly across various plant species. In apples (Malus domestica), ET promotes anthocyanin synthesis, whereas in Arabidopsis thaliana, it inhibits accumulation. Our research showed that ethephon (Eth), an derivative, ‘Viviana’ lilies, which has great potential cut flower industry. The mechanism whereby lilies remains unclear. this study, we screened and characterized ET-induced response factors (ERFs), LvERF113, with inhibitory function. analyses suggested LvERF113 could inhibit negative function LvMYB1 at transcriptional posttranslational levels, promoting lily tepals. addition, is positively regulated by LvMYB5, forming LvMYB5-LvERF113-LvMYB1 module controlling lily. These findings offer new insights into network provide a theoretical basis for application derivatives

Язык: Английский

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LvWRKY75 enhances the transcription of LvMYB5 and promotes anthocyanin biosynthesis in lily petals during the blooming phase

Physiologia Plantarum, Год журнала: 2025, Номер 177(2)

Опубликована: Март 1, 2025

Anthocyanin accumulation plays a crucial role in enhancing Lilium petal colouration; however, breeding efforts are hindered by our lack of understanding the complex molecular mechanism behind pigment's synthesis. This study explores potential WRKY family gene WRKY75 anthocyanin synthesis lilies. Contrary to inhibitory effect observed Arabidopsis thaliana, both transient silencing and overexpression analyses LvWRKY75 indicate that positively regulates The was found cause significant upregulation structural genes pivotal for biosynthesis lilies, including Lv3GT, LvDFR LvANS, as well regulatory LvMYB5. Further in-depth analyses, yeast one-hybrid, electrophoretic mobility shift assay, dual-luciferase assays, demonstrated binds promoter LvMYB5, its transcriptional activity. In turn, increased expression LvMYB5 upregulates transcription downstream such LvANS. summary, this provides deeper mechanisms contributing improving strategies flowers' ornamental value commercial appeal.

Язык: Английский

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The bHLH transcription factor AcB2 regulates anthocyanin biosynthesis in onion (Allium cepa L.)

Horticulture Research, Год журнала: 2022, Номер unknown

Опубликована: Июнь 2, 2022

Abstract Onion bulb color is a key breeding trait. The red caused by the presence of anthocyanins, which are products flavonoid synthesis pathway. Research on regulation in onion lagging compared with that other crops. AcB2 encodes bHLH transcription factor, and its positively associated anthocyanin accumulation correlated expression AcMYB1, an activator biosynthetic pathway onion. Phylogenetic analysis showed was grouped into TRANSPARENT TESTA 8 (TT8) clade IIIf subgroup. protein contained MYB-interacting region physically interacted AcMYB1 yeast tobacco leaves. directly bound to promoters anthocyanidin synthase (AcANS) 3-hydroxylase 1 (AcF3H1) activated their expression. coexpression Arabidopsis thaliana protoplasts dramatically increased AcANS AcF3H1 under alone. Transient co-overexpression induced epithelial cells bulbs. Complementation tt8-1 mutant restored pigmentation defects tt8-1. In addition, AtTT2 leaves, indicating functions were similar those AtTT8. Together, these results demonstrated enhanced function upregulating biosynthesis onion, provided theoretical basis for onions higher contents.

Язык: Английский

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Integrative analysis of transcriptome and metabolome reveals flavonoid biosynthesis regulation in Rhododendron pulchrum petals

BMC Plant Biology, Год журнала: 2022, Номер 22(1)

Опубликована: Авг. 16, 2022

Abstract Background Color is the major ornamental feature of Rhododendron genus, and it related to contents flavonoid in petals. However, regulatory mechanism biosynthesis pulchrum remains unknown. The transcriptome metabolome analysis with white, pink purple color this study aimed reveal provide insight for improving petal color. Results Flavonoids flavonols are components metabolites R.pulchrum , such as laricitrin, apigenin, tricin, luteolin, isoorientin, isoscutellarein, diosmetin their glycosides derivatives. With analysis, we found CHS, FLS, F3’H, F3′5’H, DFR, ANS GT, FNS IFR FAOMT genes showed significantly differential expression cultivar ‘Zihe'. were discovered be associated coloration first time. gene existed form FNSI. its medicarpin derivatives highly expressed petal. In ‘Fenhe', up-regulation F3’H F3′5’H down-regulation 4CL, ANS, GT coloration. transcription factor a subfamily DREBs was specifically enriched This suggested that DREB family play an important role cultivars ‘Baihe', inhibited by low CHS while pigment accumulation F3′5'H, DFR which led white Conclusions By analyzing principal pathway identified. Many novel metabolites, genes, factors have been discovered. To different petals, model constructed. These results depth information regarding petals metabolism R.pulcherum . profiling gains further genetic improvement

Язык: Английский

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Multifaceted roles of LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Lilium spp.)

Horticulture Research, Год журнала: 2023, Номер 10(9)

Опубликована: Авг. 22, 2023

The Asiatic hybrid lily (Lilium spp.) is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns. However, the regulatory mechanism underlying flower color has been largely unexplored. Here, we identified WRKY transcription factor from tepals, LhWRKY44, whose expression was closely associated accumulation. Functional verification indicated that LhWRKY44 positively regulated physically interacted LhMYBSPLATTER directly bound to promoter, which enhanced effect of LhMYBSPLATTER-LhbHLH2 MBW complex activator on Moreover, EMSA dual-luciferase assays revealed activated promoters gene LhF3H intracellular anthocyanin-related glutathione S-transferase LhGST. Interestingly, our further results showed participated in light drought-induced accumulation, improved drought tolerance via activating stress-related genes. These generated multifaceted for LhWRKY44-meditaed enhancement by environmental signal pathway accumulation expanded understanding WRKY-mediated transcriptional hierarchy modulating lilies.

Язык: Английский

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