Abstract
Research
on
how
plants
respond
to
hypoxia
has
concentrated
model
organisms
where
tissues
can
only
survive
hypoxic
conditions
for
a
few
hours
days.
In
contrast,
are
used
commercially
as
method
prolong
the
shelf
life
of
Malus
domestica
(apple)
fruit
up
year
storage
without
substantial
changes
in
quality,
not
mention
lack
tissue
death.
This
ability
apples
withstand
protracted
is
an
interesting
adaptation
that
had
limited
molecular
investigation
despite
its
economic
importance.
Here,
we
investigate
long‐term
apple
response
using
time‐course
RNA‐seq
analysis
several
postharvest
conditions.
We
use
phylogenetics,
differential
expression,
and
regulatory
networks
identify
genes
regulate
regulated
by
response.
potential
neofunctionalization
core‐hypoxia
apples,
including
novel
regulation
group
VII
ethylene
factor
(ERF
VII)
plant
cysteine
oxidase
(PCO)
family
members.
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
91(1)
Опубликована: Дек. 10, 2024
ABSTRACT
Arbuscular
mycorrhizal
fungi
(AMF)
are
promoted
as
commercial
bioinoculants
for
sustainable
agriculture.
Little
is
known,
however,
about
the
survival
of
AMF
inoculants
in
soil
and
their
impacts
on
native
or
pre-established
communities
root
tissue.
The
current
study
was
designed
to
assess
stability
pre-existing/nursery-derived
apple
rootstocks
after
being
planted
into
containing
a
known
community
with
limited
number
species.
Root-associated
endophytic
(bacteria
fungi)
differ
depending
rootstock
genotype.
Thus,
an
additional
aim
this
explore
effect
genotype
structure.
A
greenhouse
experiment
conducted
which
variety
genotypes
(G.890,
G.935,
M.26,
M.7)
were
inoculated
commercially
available,
multi-species
consortium.
Nursery-derived
sequenced,
changes
structure
following
cultivation
pasteurized
(inoculated
non-inoculated)
assessed
using
Glomeromycota-specific
phylogenetic
tree,
included
91
different
species
from
24
genera.
Results
show
that
inoculant
colonization
potential
serve
significant
source
inoculum
nursery
where
they
produced.
Rootstocks
established
relationships
introduced
genotype-specific
manner.
Regardless
success,
caused
alterations
resident
both
Geneva
Malling
rootstocks,
particularly
low
abundance
taxa.
In
addition,
phylogeny-based
analysis
revealed
unique,
well-supported
clade
unknown
taxonomy,
highlighting
importance
phylogenetic-based
classification
accurate
characterization
communities.
IMPORTANCE
Understanding
residential
essential
improving
plant
productivity
orchard
systems.
general,
there
dearth
data
interactions
living
symbiosis
host
plant.
interplay
between
microbiome
also
area
more
research
needed.
This
demonstrates
nursery-established
associations
be
maintained
when
transplanted
field.
addition
providing
insight
rootstock/AMF
associations,
our
calls
attention
issues
attendant
relying
web-based
databases
determining
identity.
use
tools
represents
one
possible
solution
may
value
industry
practitioners
terms
product
composition
consistency.
Abstract
Research
on
how
plants
respond
to
hypoxia
has
concentrated
model
organisms
where
tissues
can
only
survive
hypoxic
conditions
for
a
few
hours
days.
In
contrast,
are
used
commercially
as
method
prolong
the
shelf
life
of
Malus
domestica
(apple)
fruit
up
year
storage
without
substantial
changes
in
quality,
not
mention
lack
tissue
death.
This
ability
apples
withstand
protracted
is
an
interesting
adaptation
that
had
limited
molecular
investigation
despite
its
economic
importance.
Here,
we
investigate
long‐term
apple
response
using
time‐course
RNA‐seq
analysis
several
postharvest
conditions.
We
use
phylogenetics,
differential
expression,
and
regulatory
networks
identify
genes
regulate
regulated
by
response.
potential
neofunctionalization
core‐hypoxia
apples,
including
novel
regulation
group
VII
ethylene
factor
(ERF
VII)
plant
cysteine
oxidase
(PCO)
family
members.
