Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Дек. 16, 2022
The
genus
Sabia
is
a
woody
climber
belonging
to
the
family
Sabiaceae,
order
Proteales.
Several
species
of
this
have
been
utilized
as
medicines
for
treating
diseases,
such
rheumatic
arthritis,
traumatism,
hepatitis,
etc
.
However,
lack
molecular
data
has
prevented
accurate
identification
and
refinement
taxonomic
relationships
in
genus.
In
study,
chloroplast
genomes
11
samples
were
assembled
analyzed.
These
showed
typical
quadripartite
structure
ranged
length
from
160,956
162,209
bp.
was
found
be
relatively
conserved,
with
130
genes
annotated,
including
85
coding
genes,
37
tRNA
eight
rRNA
genes.
A
total
78–98
simple
sequence
repeats
52–61
interspersed
detected.
Sequence
alignment
revealed
highly
variable
loci
genomes.
Among
these
loci,
ndhF
-
ndhD
achieved
remarkably
higher
resolution
than
other
regions.
addition,
phylogenetic
analysis
indicated
that
Sect.
Pachydiscus
did
not
form
two
separate
monophyletic
groups.
divergence
time
calculated
based
on
Reltime
method
evolutionary
branches
Meliosma
started
approximately
85.95
million
years
ago
(Mya),
within
began
diverge
7.65
Mya.
conclusion,
our
study
provides
basis
comprehensively
exploring
It
also
methodological
support
establishing
standardized
scientific
system
The
processed
tuberous
roots
of
Asparagus
cochinchinensis
(Asparagaceae),
known
as
Asparagi
Radix,
have
long
been
used
in
East
Asia
(particularly
China)
traditional
medicines
and
play
an
indispensable
role
the
pharmaceutical
industry.
However,
frequent
adulteration
Radix
with
obtained
from
nine
other
congeneric
species
could
potentially
compromise
quality
control
measures
for
related
products,
while
also
posing
challenges
to
conservation
rational
exploitation
adulterant
that
are
ethnomedicines.
Given
this
issue,
study
aims
develop
a
molecular
authentication
method
accurate
identification
A.
adulterants,
employing
genome
skimming
approach
generate
complete
plastid
genomes
(plastomes)
nuclear
ribosomal
DNA
(nrDNA)
arrays
candidate
markers.
Through
comprehensive
phylogenetic
genetic
distance
analyses
based
on
extensive
sampling
at
both
inter-
intra-specific
levels,
efficacy
two
markers
was
assessed
by
investigating
whether
their
inter-specific
divergences
align
taxonomically
delineated
boundaries.
results
indicated
plastomes
exhibit
superior
performance
accurately
identifying
(the
botanical
source
Radix)
thus
can
serve
optimal
effective
Radix.
desirable
discriminative
power
demonstrated
suggests
PCR-free
developed
will
not
only
contribute
products
derived
but
facilitate
efforts
commonly
adulterants.
Molecular Ecology Resources,
Год журнала:
2022,
Номер
22(8), С. 3161 - 3175
Опубликована: Июль 5, 2022
Plastid
genome
and
nuclear
ribosomal
DNA
(nrDNA)
arrays,
proposed
recently
as
"super-barcodes,"
might
provide
additional
discriminatory
power
overcome
the
limitations
of
traditional
barcoding
loci,
yet
super-barcodes
need
to
be
tested
for
their
effectiveness
in
more
plant
groups.
Morphological
homoplasy
among
Schima
species
makes
genus
a
model
testing
efficacy
super-barcodes.
In
this
study,
we
generated
multiple
data
sets
comprising
standard
barcodes
(matK,
rbcL,
trnH-psbA,
nrITS)
(plastid
genome,
nrDNA
arrays)
across
58
individuals
from
12
out
13
China.
No
samples
were
correctly
assigned
using
while
only
27.27%
with
accessions
distinguished
plastid
its
partitioned
sets-the
lowest
estimated
rate
super-barcode
success
literature
so
far.
For
other
taxa
similarly
divergence
low
levels
genetic
variation,
incomplete
lineage
sorting,
hybridization
or
taxonomic
oversplitting
are
all
possible
causes
failure.
Taken
together,
our
study
suggests
that
by
no
means
immune
challenges
imposed
evolutionary
complexity.
We
therefore
call
developing
multilocus
markers
discrimination
Abstract
Background
The
Ferula
genus
encompasses
180–185
species
and
is
one
of
the
largest
genera
in
Apiaceae,
with
many
possessing
important
medical
value.
previous
studies
provided
more
information
for
,
but
its
infrageneric
relationships
are
still
confusing.
In
addition,
genetic
basis
adaptive
evolution
remains
poorly
understood.
Plastid
genomes
variable
sites
have
potential
to
reconstruct
robust
phylogeny
plants
investigate
plants.
Although
chloroplast
been
reported
within
genus,
few
conducted
using
genomes,
especially
endemic
China.
Results
Comprehensively
comparative
analyses
22
newly
sequenced
assembled
plastomes
indicated
that
these
had
highly
conserved
genome
structure,
gene
number,
codon
usage,
repeats
type
distribution,
varied
size,
GC
content,
SC/IR
boundaries.
Thirteen
mutation
hotspot
regions
were
detected
they
would
serve
as
promising
DNA
barcodes
candidates
identification
related
genera.
Phylogenomic
high
supports
resolutions
showed
Talassia
transiliensis
Soranthus
meyeri
nested
thus
should
be
transferred
into
genus.
Our
phylogenies
also
monophyly
subgenera
Sinoferula
Narthex
Twelve
genes
significant
posterior
probabilities
identified
positively
selective
analysis,
their
function
may
relate
photosystem
II,
ATP
subunit,
NADH
dehydrogenase.
Most
them
might
play
an
role
help
adapt
high-temperatures,
strong-light,
drought
habitats.
Conclusion
Plastome
data
powerful
efficient
improve
support
resolution
complicated
phylogeny.
helpful
harsh
environment.
Overall,
our
study
supplies
a
new
perspective
comprehending
.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Март 18, 2024
Abstract
Aeluropus
littoralis
,
a
halophyte
grass,
is
widely
distributed
from
the
Mediterranean
to
Indian
subcontinent
through
Mongolian
Gobi.
This
model
has
garnered
increasing
attention
owing
its
use
as
forage
and
high
tolerance
environmental
stressors.
The
chloroplast
genomes
of
many
plants
have
been
extensively
examined
for
molecular,
phylogenetic
transplastomic
applications.
However,
no
published
research
on
A.
(cp)
genome
was
discovered.
Here,
entire
assembled
implementing
accurate
long-read
sequences.
genome,
with
an
estimated
length
135,532
bp
(GC
content:
38.2%),
quadripartite
architecture
includes
pair
inverted
repeat
(IR)
regions,
IRa
IRb
(21,012
each),
separated
by
large
small
single-copy
regions
(80,823
12,685
bp,
respectively).
features
consist
133
genes
that
synthesize
87
peptides,
38
transfer
RNAs,
8
ribosomal
RNAs.
Of
these
genes,
86
were
unique,
whereas
19
duplicated
in
IR
regions.
Additionally,
total
forty-six
simple
sequence
repeats,
categorized
into
32-mono,
four-di,
two-tri,
eight-tetranucleotides,
Furthermore,
ten
sets
repeats
greater
than
20
located
primarily
LSC
region.
Evolutionary
analysis
based
data
revealed
lagopoides
sinensis
belong
Aeluropodinae
subtribe,
which
sister
Eleusininae
tribe
Cynodonteae
subfamily
Chloridoideae.
belongs
PACMAD
clade,
contains
majority
C4
photosynthetic
Poaceae.
newly
constructed
cp
offers
valuable
knowledge
DNA
barcoding,
phylogenetic,
research,
other
biological
studies.
Fritillariae
Cirrhosae
Bulbus
(FCB)
is
frequently
adulterated
with
its
closely
related
species
due
to
personal
or
non-man
made
factors,
leading
alterations
in
the
composition
of
constituents
and
compromising
efficacy
products.
