Integrated Transcriptomic and Metabolomics Analyses Reveal Molecular Responses to Cold Stress in Coconut (Cocos nucifera L.) Seedlings

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(19), С. 14563 - 14563

Опубликована: Сен. 26, 2023

Coconut is an important tropical and subtropical fruit oil crop severely affected by cold temperature, limiting its distribution application. Thus, studying low-temperature reaction mechanism required to expand cultivation range. We used growth morphology physiological analyses characterize the response of coconuts 10, 20, 30 d low temperatures, combined with transcriptome metabolome analysis. Low-temperature treatment significantly reduced plant height dry weight coconut seedlings. The contents soil analyzer development (SPAD), soluble sugar (SS), protein (SP), proline (Pro), malondialdehyde (MDA) in leaves were increased, along activities superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), endogenous hormones abscisic acid (ABA), auxin (IAA), zeatin (ZR), gibberellin (GA) contents. A large number differentially expressed genes (DEGs) (9968) detected under conditions. Most DEGs involved mitogen-activated kinase (MAPK) signaling pathway-plant, hormone signal transduction, plant-pathogen interaction, biosynthesis amino acids, nucleotide metabolism, carbon starch sucrose purine phenylpropanoid pathways. Transcription factors (TFs), including WRKY, AP2/ERF, HSF, bZIP, MYB, bHLH families, induced express stress. In addition, most associated major cold-tolerance pathways, such as ICE-CBF-COR, MAPK signaling, their up-regulated. Under a total 205 accumulated metabolites (DAMs) enriched; 206 DAMs positive-ion mode 97 negative-ion mode, mainly phenylpropanoids polyketides, lipids lipid-like molecules, benzenoids, organoheterocyclic compounds, organic oxygen acids derivatives, nucleosides, nucleotides, analogues. Comprehensive analysis revealed that related enriched acid, flavonoid, carbohydrate, lipid, metabolism pathways Together, results this study provide insights into stress, which will reveal underlying molecular mechanisms help screening breeding.

Язык: Английский

---

Transcriptomic and metabolomic analysis reveal the cold tolerance mechanism of common beans under cold stress

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 15, 2025

Common bean (Phaseolus vulgaris L.) is a thermophilic crop, and exposure to cold stress can significantly impact their yield quality. To elucidate the of on cold-tolerant 'Wei Yuan' (WY) cold-sensitive 'Bai Bu Lao' (BBL) common bean, mechanism tolerance was studied by physiological biochemical multi-omics analysis. In this study, lower relative conductivity higher malondialdehyde content after endowed 'WY' seedlings with tolerance. A total 11,837 differentially expressed genes (DEGs) 923 differential metabolites (DEMs) were identified transcriptome metabolomics Joint analysis showed that under stress, DEGs DEMs in beans are extensively engaged sugar, amino acid isoflavonoid biosynthesis, flavone flavonol plant hormone signal translation, especially related isoflavone biosynthesis. addition, it also found bHLH MYB family transcription factors may be involved transduction bean. The above results will provide theoretical basis for help screening resources beans. Not applicable.

Язык: Английский

---

Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Апрель 10, 2024

Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces Guanxi autonomous region of China. However, trees growing these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolome transcriptome profiles leaves are limited. Here, we used combined analyses three mango cultivars with different tolerance, i.e. Jinhuang (J)-tolerant, Tainung (T) Guiremang No. 82 (G)-susceptible, after 24 (LF), 48 (MF) 72 (HF) hours cold.

Язык: Английский

---

Revitalizing agriculture: next-generation genotyping and -omics technologies enabling molecular prediction of resilient traits in the Solanaceae family

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Фев. 5, 2024

This review highlights -omics research in Solanaceae family, with a particular focus on resilient traits. Extensive has enriched our understanding of genomics and genetics, historical varietal development mainly focusing disease resistance cultivar improvement but shifting the emphasis towards unveiling resilience mechanisms genebank-preserved germplasm is nowadays crucial. Collecting such information, might help researchers breeders developing new experimental design, providing an overview state art most advanced approaches for identification genetic elements laying behind resilience. Building this starting point, we aim at useful tool tackling global agricultural goals these crops.

Язык: Английский

---

Capsicum Leaves under Stress: Using Multi-Omics Analysis to Detect Abiotic Stress Network of Secondary Metabolism in Two Species

Antioxidants, Год журнала: 2022, Номер 11(4), С. 671 - 671

Опубликована: Март 30, 2022

The plant kingdom contains an enormous diversity of bioactive compounds which regulate growth and defends against biotic abiotic stress. Some these compounds, like flavonoids, have properties are health supporting relevant for industrial use. Many valuable synthesized in various pepper (Capsicum sp.) tissues. Further, a huge amount biomass residual remains from production after harvest, provides important opportunity to extract metabolites optimize the utilization crops. Moreover, stresses induce synthesis such as defense mechanism. Two different Capsicum species were therefore exposed chilling temperature (24/18 ℃ vs. 18/12 ℃), salinity (200 mM NaCl), or combination thereof 1, 7 14 days investigate effect on metabolome transcriptome profiles their leaves. Both both responded all with increase over time. All resulted repression photosynthesis genes. Stress involving induced secondary metabolism whereas salt repressed cell wall modification solute transport. analysis annotated putatively many stimulating flavonoids (apigetrin, rutin, kaempferol, luteolin quercetin) residuals, response salinity, thereof, supported by related structural genes network analysis.

Язык: Английский

---

CaSPDS, a Spermidine Synthase Gene from Pepper (Capsicum annuum L.), Plays an Important Role in Response to Cold Stress

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(5), С. 5013 - 5013

Опубликована: Март 6, 2023

Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. SPDS genes help regulate plant response to environmental stresses, but their roles pepper remain unclear. In this study, we identified and cloned gene from (Capsicum annuum L.), named CaSPDS (LOC107847831). Bioinformatics analysis indicated that contains two highly conserved domains: an tetramerisation domain spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed was expressed stems, flowers, mature fruits of rapidly induced by cold stress. The function stress studied silencing overexpressing it Arabidopsis, respectively. Cold injury more serious reactive oxygen species levels were greater CaSPDS-silenced seedlings than wild-type (WT) after treatment. Compared with WT plants, CaSPDS-overexpression Arabidopsis plants tolerant higher antioxidant activities, spermidine content, cold-responsive (AtCOR15A, AtRD29A, AtCOR47, AtKIN1) expression. These indicate plays important valuable molecular breeding enhance tolerance pepper.

Язык: Английский

---

Integration of transcriptome, volatile and non-volatile metabolite profile reveals characteristic aroma formation in Toona sinensis

Food Chemistry, Год журнала: 2023, Номер 436, С. 137788 - 137788

Опубликована: Окт. 17, 2023

Язык: Английский

---

Genome-wide analysis and identification of stress-responsive genes of the CCCH zinc finger family in Capsicum annuum L.

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Май 30, 2023

The CCCH zinc finger gene family encodes a class of proteins that can bind to both DNA and RNA, an increasing number studies have demonstrated the plays key role in growth development responses environmental stress. Here, we identified 57 genes pepper (Capsicum annuum L.) genome explored evolution function C. annuum. Substantial variation was observed structure these genes, exons ranged from one fourteen. Analysis duplication events revealed segmental main driver expansion pepper. We found expression significantly up-regulated during response biotic abiotic stress, especially cold heat indicating play roles stress responses. Our results provide new information on will aid future evolution, inheritance,

Язык: Английский

---

Unveiling tolerance mechanisms in pepper to combined low-temperature and low-light stress: a physiological and transcriptomic approach

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 10, 2025

Pepper (Capsicum annuum L.) is a vegetable crop of significant economic importance, but its yield and quality are severely affected by the combined stress low temperature light (LL), particularly in greenhouse environments. Despite this, physiological molecular mechanisms underlying pepper's response to LL remain poorly understood. In this study, we conducted transcriptomic analyses on two pepper genotypes: Y2, LL-sensitive genotype, Y425, LL-tolerant genotype. These genotypes were subjected conditions (10 °C/5°C, 100 µmol m⁻²s⁻¹) control (CK) (28 °C/18°C, 300 m⁻²s⁻¹). Three days after treatment, phenotypes began show clear distinctions, with Y425 seedlings exhibiting greater root length, shoot fresh weight, weight compared Y2. Additionally, comparative transcriptome analysis leaf samples from both identified total 13,190 differentially expressed genes (DEGs). Gene Ontology (GO) enrichment revealed that associated photosynthesis, osmotic response, reactive oxygen species other GO terms potentially contribute tolerance. Moreover, three key pathways involved identified: photosynthesis-antenna proteins, zeatin biosynthesis, circadian rhythm pathways. The DEGs these at higher levels as Furthermore, indicators such chlorophyll fluorescence parameters, content, osmoregulatory substances, antioxidant enzyme activities decreased under stress; however, reduction was significantly Y2 further validating findings analysis. This study differences stress. It highlighted provide novel insights into results emphasize importance optimizing for better productivity.

Язык: Английский

---

Basil chilling injury: Oxidative stress or energy depletion?

Food Chemistry, Год журнала: 2025, Номер 477, С. 143581 - 143581

Опубликована: Фев. 25, 2025

Язык: Английский

---