Fruit Research,
Год журнала:
2022,
Номер
2(1), С. 1 - 11
Опубликована: Янв. 1, 2022
One
application
of
DNA-informed
breeding,
which
has
potential
to
increase
the
effectiveness
traditional
breeding
methods,
is
use
DNA-based
diagnostic
tests
estimate
genetic
individuals.
In
sweet
cherry
(Prunus
avium
L.),
cracked
or
soft
fruit
are
major
industry
challenges.
Recent
research
detected
two
quantitative
trait
loci
(QTLs)
for
cracking
and
firmness
differing
in
levels
associated
with
QTL
haplotypic
variation.
Also,
a
DNA
test
(Pav-G5Crack-SSR),
using
single
simple
sequence
repeat
(SSR)
marker,
was
previously
developed
but
not
yet
validated
on
germplasm.
addition
SSR
markers,
nucleotide
polymorphism
(SNP)
markers
can
be
used
developing
locus-specific
run
as
assays
such
high-resolution
melting
(HRM).
The
objective
this
develop
evaluate
predictiveness
cherry.
Unselected
seedlings
from
pedigree-connected
families
were
screened
Pav-G5Crack-SSR
test.
also
created
four
SNP
HRM
assays,
years
data
evaluation.
explained
12–15%
phenotypic
variance,
while
Pav-G1Crack-SNP
Pav-G5Crack-SNP
(which
targeted
same
Pav-G5Crack-SSR)
together
16–30%
variance.
Pav-G1Firm-SNP
Pav-G3Firm-SNP
22–28%
All
three
implemented
programs
enhance
decreased
incidence
increased
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 18, 2024
Introduction
Prickles
are
often
deemed
undesirable
traits
in
many
crops,
including
roses
(
Rosa
sp.),
and
there
is
demand
for
rose
cultivars
with
no
or
very
few
prickles.
This
study
aims
to
identify
new
and/or
validate
reported
quantitative
trait
loci
(QTLs)
associated
stem
leaf
rachis
prickle
density,
characterize
the
effects
of
functional
haplotypes
major
QTLs,
sources
QTL-alleles
increased/decreased
density
roses.
Methods
QTL
mapping
using
pedigree-based
analysis
(PBA),
haplotype
were
conducted
on
two
multi-parental
diploid
populations
(TX2WOB
TX2WSE).
Results
discussion
Twelve
QTLs
identified
linkage
groups
(LGs)
2,
3,
4,
6.
The
located
between
42.25
45.66
Mbp
chromosome
3
chinensis
genome
assembly,
individual
explaining
18
49%
phenotypic
variance
(PVE).
remaining
mapped
minor.
As
several
detected
LG3,
6
PVE
8
17%.
Also,
this
that
ancestors
R.
wichurana
‘Basye’s
Thornless’,
‘Old
Blush’,
pollen
parent
M4-4
common
favorable
alleles
q
)
decreased
whereas
’Little
Chief’
‘Srche
Europy’
source
unfavorable
Q
TX2WOB
TX2WSE
populations,
respectively.
outcomes
work
complement
other
studies
locate
factors
affect
density.
These
results
can
also
be
utilized
develop
high-throughput
DNA
tests
apply
parental
selection
prickle-free
cultivars.
Fruit Research,
Год журнала:
2022,
Номер
2(1), С. 1 - 11
Опубликована: Янв. 1, 2022
One
application
of
DNA-informed
breeding,
which
has
potential
to
increase
the
effectiveness
traditional
breeding
methods,
is
use
DNA-based
diagnostic
tests
estimate
genetic
individuals.
In
sweet
cherry
(Prunus
avium
L.),
cracked
or
soft
fruit
are
major
industry
challenges.
Recent
research
detected
two
quantitative
trait
loci
(QTLs)
for
cracking
and
firmness
differing
in
levels
associated
with
QTL
haplotypic
variation.
Also,
a
DNA
test
(Pav-G5Crack-SSR),
using
single
simple
sequence
repeat
(SSR)
marker,
was
previously
developed
but
not
yet
validated
on
germplasm.
addition
SSR
markers,
nucleotide
polymorphism
(SNP)
markers
can
be
used
developing
locus-specific
run
as
assays
such
high-resolution
melting
(HRM).
The
objective
this
develop
evaluate
predictiveness
cherry.
Unselected
seedlings
from
pedigree-connected
families
were
screened
Pav-G5Crack-SSR
test.
also
created
four
SNP
HRM
assays,
years
data
evaluation.
explained
12–15%
phenotypic
variance,
while
Pav-G1Crack-SNP
Pav-G5Crack-SNP
(which
targeted
same
Pav-G5Crack-SSR)
together
16–30%
variance.
Pav-G1Firm-SNP
Pav-G3Firm-SNP
22–28%
All
three
implemented
programs
enhance
decreased
incidence
increased
