Editing VvDXS1 for the creation of muscat flavour in Vitis vinifera cv. Scarlet Royal

Plant Biotechnology Journal, Год журнала: 2024, Номер 22(6), С. 1610 - 1621

Опубликована: Янв. 20, 2024

Muscat flavour represents a group of unique aromatic attributes in some grape varieties. Biochemically, berries with muscat produce high levels monoterpenes. Monoterpene biosynthesis is mainly through the DOXP/MEP pathway, and VvDXS1 encodes first enzyme this plastidial pathway terpene grapevine. A single-point mutation resulting substitution lysine an asparagine at position 284 protein has previously been identified as major cause for producing grapes. In study, same was successfully created prime editing table Vitis vinifera cv. 'Scarlet Royal'. The targeted point detected most transgenic vines, varying efficiencies. No unintended mutations were edited alleles, either by PCR Sanger sequencing or amplicon sequencing. More than dozen vines efficiency more 50%, indicating that these likely derived from single cells which one allele edited. These had much higher monoterpenes their leaves control, similar to what found leaf samples between field-grown non-muscat

Язык: Английский

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Greater aperture counteracts effects of reduced stomatal density on water use efficiency: a case study on sugarcane and meta-analysis

Journal of Experimental Botany, Год журнала: 2024, Номер 75(21), С. 6837 - 6849

Опубликована: Июль 17, 2024

Abstract Stomata regulate CO2 and water vapor exchange between leaves the atmosphere. are a target for engineering to improve crop intrinsic use efficiency (iWUE). One example is by expressing genes that lower stomatal density (SD) reduce conductance (gsw). However, quantitative relationship reduced SD, gsw, mechanisms underlying it poorly understood. We addressed this knowledge gap using low-SD sugarcane (Saccharum spp. hybrid) as case study alongside meta-analysis of data from 10 species. Transgenic expression EPIDERMAL PATTERNING FACTOR 2 Sorghum bicolor (SbEPF2) in SD 26–38% but did not affect gsw compared with wild type. Further, no changes occurred complex size or proxies photosynthetic capacity. Measurements gas at low concentrations promote complete opening normalize aperture genotypes were combined modeling maximum anatomical data. These suggest increased only possible explanation maintaining when reduced. Meta-analysis across C3 dicots, monocots, C4 monocots revealed engineered reductions strongly correlated (r2=0.60–0.98), response damped relative change anatomy.

Язык: Английский

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CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come

Horticulturae, Год журнала: 2024, Номер 10(9), С. 965 - 965

Опубликована: Сен. 11, 2024

The advent of Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated (Cas) proteins as a revolutionary innovation in genome editing has greatly promoted targeted modification and trait improvement most plant species. For grapevine (Vitis vinifera L.), perennial woody species, CRISPR/Cas is an extremely promising technique for genetic short period. Advances have been achieved by using CRISPR technology recent years, which promises to accelerate grapevine. In this review, we describe the development advances CRISPR/Cas9 its orthologs variants. We summarize applications discuss challenges facing well possible strategies that could be used improve addition, outline future perspectives model system, precise editing, accelerated improvement, transgene-free editing. believe will play more important role exciting bright expected economically significant

Язык: Английский

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The Potential of CRISPR/Cas Technology to Enhance Crop Performance on Adverse Soil Conditions

Plants, Год журнала: 2023, Номер 12(9), С. 1892 - 1892

Опубликована: Май 5, 2023

Worldwide food security is under threat in the actual scenery of global climate change because major staple crops are not adapted to hostile climatic and soil conditions. Significant efforts have been performed maintain yield crops, using traditional breeding innovative molecular techniques assist them. However, additional strategies necessary achieve future demand. Clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas) technology, as well its variants, emerged alternatives transgenic plant breeding. This novelty has helped accelerate modifications confront impact abiotic stress on agriculture systems. review summarizes current advances CRISPR/Cas applications deal with main conditions, such drought, flooding waterlogging, salinity, heavy metals, nutrient deficiencies. In addition, potential extremophytes a reservoir new mechanisms for tolerance, their orthologue identification edition shown. Moreover, challenges prospects related technology issues, legal regulations, customer acceptance will be discussed.

Язык: Английский

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Application of new breeding techniques in fruit trees

PLANT PHYSIOLOGY, Год журнала: 2023, Номер unknown

Опубликована: Июль 3, 2023

Abstract Climate change and rapid adaption of invasive pathogens pose a constant pressure on the fruit industry to develop improved varieties. Aiming accelerate development better-adapted cultivars, new breeding techniques have emerged as promising alternative meet demand growing global population. Accelerated breeding, cisgenesis, CRISPR/Cas genome editing hold significant potential for crop trait improvement proven be useful in several plant species. This review focuses successful application these technologies trees confer pathogen resistance tolerance abiotic stress improve quality traits. In addition, we optimization diversification tools applied trees, such multiplexing, CRISPR/Cas-mediated base site-specific recombination systems. Advances protoplast regeneration delivery techniques, including use nanoparticles viral-derived replicons, are described obtention exogenous DNA-free tree The regulatory landscape broader social acceptability cisgenesis also discussed. Altogether, this provides an overview versatility applications improvement, well current challenges that deserve attention further implementation techniques.

Язык: Английский

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Transgene-free Genome Editing in Grapevine

BIO-PROTOCOL, Год журнала: 2025, Номер 15(1365)

Опубликована: Янв. 1, 2025

CRISPR/Cas9 genome editing technology has revolutionized plant breeding by offering precise and rapid modifications. Traditional methods are often slow imprecise, whereas allows for targeted genetic improvements. Previously, direct delivery of Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) complexes to grapevine (Vitis vinifera) protoplasts been demonstrated, but successful regeneration edited into whole plants not achieved. Here, we describe an efficient protocol obtaining transgene/DNA-free transfecting isolated from embryogenic callus subsequently regenerating them. The regenerated were comparable in morphology growth habit wild-type controls. This provides a highly method DNA-free grapevine, addressing regulatory concerns potentially facilitating the improvement other woody crop plants. Key features • Protoplasts one most commonly used systems application new technologies, including editing. accessible platform CRISPR-Cas9 ribonucleoparticles through chemical or physical transfection. avoid use both Agrobacterium tumefaciens plasmids; no stable integration exogenous DNA occurs. background remains unchanged identical original plant.

Язык: Английский

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Somatic embryogenesis and Agrobacterium-mediated transformation in a number of grape cultivars

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2025, Номер 160(3)

Опубликована: Март 1, 2025

Язык: Английский

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DSD1/ZmICEb regulates stomatal development and drought tolerance in maize

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

ABSTRACT Maize ( Zea mays L.) growth and yield are severely limited by drought stress worldwide. Stomata play crucial roles in transpiration gas exchange thus essential for improving plant water‐use efficiency (WUE) to help plants deal with the threat of drought. In this study, we characterized maize dsd1 decreased stomatal density 1 ) mutant, which showed defects development, including guard mother cell differentiation, subsidiary formation maturation. DSD1 encodes basic helix‐loop‐helix transcription factor INDUCER OF CBF EXPRESSION b (ZmICEb) is a homolog ICE1 Arabidopsis thaliana ). DSD1/ZmICEb expressed file cells throughout development plays conserved role across Arabidopsis. Mutations dramatically improved tolerance WUE reduced losses under conditions. Therefore, represents promising candidate target gene genetic improvement manipulating density.

Язык: Английский

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Genome Editing in Improving Photosynthesis in Crop Plants

CABI eBooks, Год журнала: 2025, Номер unknown, С. 217 - 246

Опубликована: Март 21, 2025

Язык: Английский

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Help from the past to cope with the future: Vitis sylvestris as a resource for abiotic stress resilience

Plants People Planet, Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Societal Impact Statement Viticulture is facing increasing challenges due to climate change. The focus on fast growth and sweet berries has come at the expense of stress resilience. Grafting onto Phylloxera‐resistant rootstocks from American species been most successful form ecological pest management. However, there still a significant reliance chemical plant protection. Additionally, abiotic not primary concern in rootstock breeding efforts so far. To identify genetic factors that contribute tolerance, we propose explore potential wild ancestor grapevine, Vitis sylvestris . By identifying resilience factors, can develop new generation or enhance grafted cultivars protect viticulture impact constraints. Summary There an urgent need germplasm resources for traits tolerance grapevines. posed by change, including heat drought stress, salinity, rising temperatures, untimely cold snaps spring, are intensifying. Traditional grapevine varieties often lack withstand environmental threats because conventional historically prioritized yield flavor over tolerance. In this review, highlight European Wild Grapevine, , as valuable resource traits. Understanding underlying mechanisms crucial developing molecular markers support breeding. Such be directly integrated through introgression into productive cultivars. Alternatively, they used scion stresses without compromising desirable oenological qualities. These may development gene editing strategies engineer more resilient genotypes.

Язык: Английский

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