Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin–abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense
Horticulture Research,
Год журнала:
2024,
Номер
11(5)
Опубликована: Март 12, 2024
Abstract
The
seasonal
flowering
Chinese
Cymbidium
produce
an
axillary
floral
meristem
and
require
a
dormancy
period
during
cold
conditions
for
flower
development.
However,
the
bud
activation
mechanism
remains
elusive.
This
study
evaluates
multi-omics
across
six
stages
of
development,
along
with
functional
analysis
core
genes
to
decipher
innate
initiation
outgrowth
in
orchid
sinense.
Transcriptome
proteome
analyses
identified
10
modules
essential
roles
activation.
Gene
clusters
early
development
were
mainly
related
time
regulation
determination,
while
late
correlated
hormone
signaling
pathways.
metabolome
69
potential
hormones
which
gibberellin
(GA)
abscisic
acid
(ABA)
main
regulatory
hubs,
GA4
GA53
exhibited
reciprocal
loop.
Extraneous
GA
application
caused
rapid
elongation
buds
promoted
expression
genes.
Contrarily,
exogenous
ABA
extended
process
inhibitors
induced
release.
Moreover,
CsAPETALA1
(CsAP1)
was
as
target
Transformation
CsAP1
Arabidopsis
its
transient
overexpression
C.
sinense
protoplasts
not
only
affected
organ
morphogenesis
but
also
orchestrated
presence
response
elements
promoter,
downregulation
after
application,
inhibitor
treatment
suggest
that
can
control
through
CsAP1.
Язык: Английский
Detection of a bibenzyl core scaffold in 28 common mangrove and associate species of the Indian Sundarbans: potential signature molecule for mangrove salinity stress acclimation
Frontiers in Plant Science,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 16, 2024
Bibenzyl
derivatives
comprising
two
benzene
rings
are
secondary
plant
metabolites
with
significant
therapeutic
value.
To
date,
bibenzyl
in
the
Plant
kingdom
have
been
primarily
identified
bryophytes,
orchids,
and
Cannabis
sativa
.
The
metabolic
cost
investment
by
species
for
synthesis
of
these
bioactive
is
rationalized
as
a
mechanism
defense
response
to
oxidative
stress
induced
biotic/abiotic
factors.
synthesized
from
core
phenylpropanoid
biosynthetic
pathway
offshoots
species.
Mangrove
mangrove
associate
thrive
under
extreme
ecological
niches
such
hypersaline
intertidal
environment
through
unique
adaptive
acclimative
characteristics,
involving
osmotic
adjustments
followed
abatement.
Several
primary/secondary
components
salinity
adaptation/acclimation/mitigation;
however,
existence
scaffold
functioning
this
context
remains
unknown.
We
here
report
confirmed
detection
extensive
gas
chromatography-mass
spectrometry
chromatography-flame
ionization
analyses
28
Indian
Sundarbans.
speculate
that
common
presence
molecule
may
be
related
its
via
branches
high
salinity,
which
functions
detoxify
reactive
oxygen
protection
maintenance
processes.
This
finding
reveals
new
eco-physiological
functional
role
bibenzyls
ecosystem.
Язык: Английский
Deciphering the complete CDS sequence of Bibenzyl synthase (BBS) gene in Pholidota articulata and its transcriptional regulation under different stress conditions
Plant Gene,
Год журнала:
2025,
Номер
unknown, С. 100491 - 100491
Опубликована: Янв. 1, 2025
Язык: Английский
Isolation, bioactivity and molecular docking of stilbene acetylcholinesterase inhibitors from Arundina graminifolia (D. Don) Hochr.
Journal of Molecular Structure,
Год журнала:
2025,
Номер
unknown, С. 141745 - 141745
Опубликована: Фев. 1, 2025
Язык: Английский
Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Март 18, 2024
Introduction
Paphiopedilum
barbigerum
is
currently
the
rarest
and
most
endangered
species
of
orchids
in
China
has
significant
ornamental
value.
The
mature
seeds
P.
are
difficult
to
germinate
owing
absence
an
endosperm
highly
dependent
on
mycorrhizal
fungi
for
germination
subsequent
development.
However,
little
known
about
regulation
mechanisms
symbiosis
symbiotic
seeds.
Methods
Herein,
transcriptomics
proteomics
were
used
explore
changes
after
inoculation
with
(FQXY019
treatment
group)
or
without
(control
Epulorhiza
sp.
FQXY019
at
90
days
germination.
Results
Transcriptome
sequencing
revealed
that
a
total
10,961
differentially
expressed
genes
(DEGs;
2,599
upregulated
8,402
downregulated)
identified
control
groups.
These
DEGs
mainly
involved
carbohydrate,
fatty
acid,
amino
acid
metabolism.
Furthermore,
expression
levels
candidate
related
nodulin,
Ca
2+
signaling,
plant
lectins
significantly
affected
Subsequently,
tandem
mass
tag-based
quantitative
was
performed
recognize
proteins
(DEPs),
537
DEPs
(220
317
enriched
processes
including
photosynthesis,
photosynthesis-antenna
proteins,
biosynthesis
Discussion
This
study
provides
novel
insight
underlying
vitro
seed
protocorm
development
by
using
compatible
fungal
symbiont
will
benefit
reintroduction
orchids.
Язык: Английский
The Integrated mRNA and miRNA Approach Reveals Potential Regulators of Flowering Time in Arundina graminifolia
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(2), С. 1699 - 1699
Опубликована: Янв. 15, 2023
Orchids
are
among
the
most
precious
flowers
in
world.
Regulation
of
flowering
time
is
one
important
targets
to
enhance
their
ornamental
value.
The
beauty
Arundina
graminifolia
its
year-round
flowering,
although
molecular
mechanism
this
ability
remains
masked.
Therefore,
we
performed
a
comprehensive
assessment
integrate
transcriptome
and
miRNA
sequencing
disentangle
genetic
regulation
valuable
species.
Clustering
analyses
provided
set
regulators
floral
transition
morphogenesis.
We
mined
candidate
homeotic
genes,
including
FCA,
FPA,
GI,
FT,
FLC,
AP2,
SOC1,
SVP,
TCP,
CO,
which
were
targeted
by
variety
miRNAs.
MiR11091
highest
number
phase
hormonal
control.
conserved
miR156-miR172
pathway
was
evident
our
data,
found
these
miRNAs
transcriptome.
Moreover,
endogenous
hormone
levels
determined
decipher
control
buds
A.
graminifolia.
qRT-PCR
analysis
integrators
validated
expression.
miRNA-mediated
mining
genes
with
forms
basis
for
comprehending
complex
regulatory
network
perpetual
orchids.
findings
study
can
do
great
deal
broaden
breeding
programs
manipulation
Язык: Английский
Arundina graminifolia (D.Don) Hochr. (Orchidaceae): A review of its medicinal importance, phytochemistry and pharmacology activities
South African Journal of Botany,
Год журнала:
2022,
Номер
150, С. 956 - 964
Опубликована: Сен. 13, 2022
Traditional
medicines
play
an
important
role
in
protecting
the
health
of
all
ethnic
groups
around
world,
and
their
importance
development
medical
services
cannot
be
overstated.
The
Orchidaceae
family
includes
some
most
beautiful
flowers
each
with
its
own
unique
shape,
color,
form.
Potted
cut
flower
orchids
are
big
business
because
bioactive
components
them
great
for
your
health.
Arundima
graminifolia
(Orchidaceae)
has
been
extensively
used
wide
range
ethnopharmacological
activities,
including
rheumatism,
food
poisoning,
snake
bites,
traumatic
injuries,
detoxification
heat
clearance.
It
is
also
utilized
to
treat
pneumonia,
tuberculosis
bronchitis
by
people.
recent
two
decades
fruitful
achievements
chemical
constituent
investigations
have
laid
a
foundation
study
therapeutically
material
basis
action
mechanism,
which
will
help
clarify
broad-spectrum
mechanism
develop
into
modern
pharmaceutical
preparation.
This
suggests
that
whole
plant
A.
more
than
other
orchid
species
extracting
constituents
such
as
flavonoids,
phenylpropanoid,
phenanthrene,
glycosides,
bibenzyl
phenolic
compounds
medicinal
purposes.
bamboo
promising
studying
efficacy
obtain
products
due
short
life
cycle
accumulation
ingredients
multiple
parts.
review
summarizes
current
usage,
phytochemistry
pharmacological
activities
this
high
value
species.
Язык: Английский
RNA Sequencing Approach for Genome-Wide Transcriptome Profiling in Ethnomedicinal Plants
R. Chandrakanth,
L. Sunil,
Sadashivaiah
и другие.
Опубликована: Янв. 1, 2024
Язык: Английский
Genetic Diversity and Its Relationship of Dendrobium (Orchidaceae) Based on Bioactive Compounds and Their Biological Activities: A Meta-Analysis
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi,
Год журнала:
2023,
Номер
33(4), С. 645 - 655
Опубликована: Дек. 15, 2023
Information
on
genetic
diversity
and
its
relationship
is
fundamental
for
the
preservation
improvement
of
orchid
germplasm.
For
Dendrobium,
such
information,
particularly
by
a
meta-analysis,
was
limited.
The
study
aimed
to
assess
relationships
Dendrobium
germplasm
based
bioactive
compounds,
their
biological
activities,
plant
organs
meta-analysis
approach.
A
total
51
species
have
been
collected
identified
as
producing
including
activities
(parts).
In
this
case,
highest
shown
polyphenols
(H'
index
=
0.90)
substances,
neuroprotective
0.80)
activity,
leaf
organ
with
an
H'
0.89.
UPGMA
analysis
showed
that
grouped
into
seven
clusters,
where
furthest
presented
D.
moschatum
catenatum.
However,
closest
relation
scabrilingue
delacourii,
snowflake
ovatum.
Following
these
parameters,
shows
unique
relationships.
Thus,
it
valuable
future
programs
Dendrobium.
Язык: Английский