Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.)
Frontiers in Plant Science,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 28, 2025
Ovule
number
per
ovary
(ONPO)
determines
the
maximum
potential
of
seed
fruit
that
is
a
direct
component
yield
in
crops.
This
study
aimed
to
dissect
genetic
basis
and
molecular
mechanism
ONPO
using
newly
developed
doubled
haploid
(DH)
population
oilseed
rape.
In
all
four
investigated
environments,
201
DH
lines
exhibited
normal
distribution
with
wide
variation
from
22.6
41.8,
suggesting
quantitative
inheritance
appropriate
for
mapping
QTL.
A
skeleton
map
2111
markers
within
19
linkage
groups
was
developed,
total
1715.71
cM
length
an
average
0.82
between
markers.
Linkage
identified
ten
QTLs
were
distributed
on
eight
chromosomes
explained
7.0-15.9%
phenotypic
variance.
Among
these,
identical
reported
two
repeatedly
detected
relatively
large
effects,
highlighting
their
marker-assisted
selection.
Phytohormone
quantification
ovaries
(at
ovule
initiation
stage)
pools
high
low
showed
significant
differences
levels
nine
sub-types
phytohormones,
important
roles
regulating
number.
Transcriptomic
analysis
7689
differentially
expressed
genes
(DEGs)
pools,
which
nearly
half
enriched
into
functional
categories
ONPO,
including
protein,
RNA,
signalling,
miscellaneous,
development,
hormone
metabolism,
tetrapyrrole
synthesis.
Integration
QTL
mapping,
transcriptome
sequencing
BLAST
15
homologues
327
DEGs
regions,
considered
as
candidate
genes.
These
findings
propose
further
insights
mechanisms
will
facilitate
future
gene
cloning
improvement
enhancing
Язык: Английский
Amino acid transporters of Brassica napus: Identification, evolution, expression and response to various stresses
Ziyi Ye,
Fei Hu,
Weimeng Zhang
и другие.
Industrial Crops and Products,
Год журнала:
2023,
Номер
194, С. 116338 - 116338
Опубликована: Фев. 20, 2023
Язык: Английский
Linkage and association mapping of ovule number per ovary (ON) in oilseed rape (Brassica napus L.)
Molecular Breeding,
Год журнала:
2023,
Номер
43(2)
Опубликована: Фев. 1, 2023
Язык: Английский
Ovule number as a rising star for regulating seed yield: Hope or hype
Journal of Integrative Agriculture,
Год журнала:
2024,
Номер
23(11), С. 3623 - 3640
Опубликована: Фев. 29, 2024
Rapeseed
(Brassica
napus
L.)
is
the
second
most
premium
oilseed
crop,
mainly
grown
for
vegetable
oil
and
protein
meal
around
globe.
One
of
main
goals
breeders
to
produce
high-yield
rapeseed
cultivars
with
sustainable
production
meet
requirements
fast-growing
population.
Besides
pod
number,
seeds
per
silique
(SS),
thousand-seed
weight
(TSW),
ovule
number
(ON)
decisive
yield
determining
factor
a
single
plant
final
seed
yield.
In
recent
years,
tremendous
efforts
have
been
made
dissect
genetic
molecular
basis
these
complex
traits.
Still,
only
relatively
few
genes
or
loci
controlling
traits
reported.
This
review
highlighted
updated
hormonal
ON
development
in
model
plants
(A.
thaliana).
also
compiled
at
hormonal,
molecular,
mechanism
bridge
understanding
between
species
thaliana)
cultivated
(B.
napus).
report
will
open
new
corridor
primary
applied
research
biology
benefit
breeding
programs.
literature
inculcate
interest
understand
further
determination,
its
role
improvement,
possible
utilization
Язык: Английский
NAC100 regulates silique growth during the initial phase of fruit development through the gibberellin biosynthetic pathway
Plant Science,
Год журнала:
2024,
Номер
unknown, С. 112344 - 112344
Опубликована: Дек. 1, 2024
The
NAC
transcription
factor
family
is
a
large
class
of
DNA-binding
proteins
found
in
several
plant
species.
In
the
model
Arabidopsis
thaliana,
factors
are
expressed
different
organs,
and
they
known
to
modulate
many
diverse
developmental
processes,
such
as
meristem
formation,
flower
fruit
development,
leaf
senescence.
From
previous
time-lapse
transcriptomic
analysis
developing
siliques
performed
by
our
group,
we
factor,
NAC100,
that
upregulated
during
silique
development.
this
work,
characterized
role
NAC100
demonstrated
contributes
regulating
growth
initial
phase
their
nac100
mutant
smaller
but
defects
can
be
rescued
through
application
exogenous
bioactive
gibberellin.
Gene
expression
analysis,
transactivation
assay
endogenous
gibberellin
quantification
indicate
modulates
metabolism,
both
directly
indirectly
GA-metabolic
genes
expression,
ultimately
affecting
elongation.
Язык: Английский
Linkage and association mapping of ovule number per ovary (ON) in oilseed rape (Brassica napus L.)
Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Окт. 25, 2022
Abstract
Ovule
number
(ON)
produced
during
flower
development
determines
the
maximum
of
seeds
per
silique
and
thereby
affects
crop
productivity;
however,
genetic
control
ON
remains
poorly
understood
in
oilseed
rape
(
Brassica
napus
).
In
this
study,
we
genetically
dissected
variations
a
double
haploid
population
(DH)
diverse
inbred
accessions
by
linkage
mapping
genome-wide
association
analysis.
Phenotypic
analysis
six
environments
showed
that
displayed
normal
distribution
both
populations
with
broad-sense
heritability
0.861
(DH
population)
0.930
(natural
population).
Linkage
identified
5
QTLs
related
to
ON,
including
qON-A03,
qON-A07,
qON-A07-2,
qON-A10
,
qON-C06
.
Genome-wide
studies
(GWAS)
revealed
214,
48
40
significant
single
nucleotide
polymorphisms
(SNPs)
individually
using
single-locus
model
GLM
multiple-locus
MrMLM
FASTMrMLM.
The
phenotypic
variation
explained
(PVE)
these
SNPs
ranged
from
2.00-17.40%
5.03–7.33%,
respectively.
Integration
results
strategies
four
consensus
genomic
regions
associated
Chr
A03,
A07,
A10.
Our
preliminarily
resolved
basis
provides
useful
molecular
markers
for
plant
yield
improvement
B.
Язык: Английский