Unraveling growth molecular mechanisms inPinus taedawith GWAS, machine learning, and gene coexpression networks DOI Creative Commons
Alexandre Hild Aono, Stephanie Karenina Bajay, Felipe Roberto Francisco

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Дек. 10, 2022

Abstract Pinus taeda (loblolly pine [LP]) is a long-lived tree species and one of the most economically significant forest species. Among growth traits, volume widely considered trait in improvement programs. However, deciphering genetic variants responsible for variations conifers, such as LP, particularly challenging due to vast size intricate complexity genomes. We present comprehensive analysis focusing on markers associated with stem variation, elucidate molecular mechanisms governing high-performance phenotypes. used population 1,692 individuals phenotyped genotyped these using sequence capture probes. To conduct genome-wide associations, we utilized both association study (GWAS) machine learning (ML) approaches. The identified were found be linked genes assembled from three distinct transcriptomes. These subsequently construct gene coexpression networks, through topological evaluations, key potential regulatory roles within configurations. Using set 31,589 SNPs, defined 7 GWAS-associated SNPs 128 ML-associated markers, all which correlated multiple involved diverse biological functions. Gene revealed group 270 potentially regulation material. Key directly implicated response stress identified, inferences about their impact development elucidated. Our not only offers insights into but also elucidates subset characterized by unique features. findings significantly advance our understanding factors influencing reveal candidate future functional studies, contribute broader comprehension architecture underlying traits LP.

Язык: Английский

Genome-Wide Identification of Rubber Tree SCAMP Genes and Functional Characterization of HbSCAMP3 DOI Creative Commons
Baoyi Yang, Xiao Huang, Yuanyuan Zhang

и другие.

Plants, Год журнала: 2024, Номер 13(19), С. 2729 - 2729

Опубликована: Сен. 29, 2024

Natural rubber produced by the tree is a vital industrial raw material globally. Seven SCAMP gene family members were identified in tree, and phylogenetic classified HbSCAMPs into three subfamilies. Significant differences observed among terms of length, number exons, composition conserved motifs. The expansion genome associated with segmental duplications. high expression HbSCAMP1–6 petioles HbSCAMP7 stem tips, along their distinct responses to drought, salt, wound stresses, indicates crucial roles substance transport stress adaptation. Transgenic poplar experiments demonstrated that overexpression HbSCAMP3 significantly promotes plant height growth, localization tobacco plasma membrane, suggesting its involvement regulating growth through membrane processes. These findings enhance understanding trees provide new insights how plants finely tune adapt environmental changes.

Язык: Английский

Процитировано

0

Genome-wide identification of protein kinase family in Populus tomentosa: Functional evidence for causative protein kinase in secondary cell wall biosynthesis DOI
Weina Qi, Mingyang Quan, Yuanyuan Fang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 285, С. 138219 - 138219

Опубликована: Ноя. 30, 2024

Язык: Английский

Процитировано

0

The Cucumis sativus kinome: Identification, annotation, and expression patterns in response to powdery mildew infection DOI Creative Commons
Francisco Cleilson Lopes Costa, Alexandre Hild Aono, Edson Mário de Andrade Silva

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 20, 2023

It is widely known that protein kinases (PKs) play a fundamental role in regulating various metabolic processes plants, from development to response the environment. However, detailed characterization of this superfamily still lacking for several species, such as cucumber (Cucumis sativus), especially regarding their involvement Powdery Mildew (PM) caused by Podosphaera xanthii. This study aimed characterize PK family, shedding light on its genomic distribution, classification, and expression patterns triggered P. The hidden Markov models (HMMs) analysis uncovered 835 PKs kinome, distributed across seven chromosomes, categorized into 20 distinct groups 123 families, with RLK group being most abundant. Evidence tandem duplication genes was also observed, enriching our understanding PKs. To investigate profiles cucumber, we analyzed transcription levels all RNA-seq data leaves resistant susceptible cultivars xanthii, which were artificially inoculated. Depending treatment, DEGs ranged 319 1,690, ranging 8 105. number varied between different contrasts analyzed. Notably, observed greater genotypes when challenged pathogen. Our findings indicate specific context plant-pathogen interactions pave way further research intricate mechanisms underlying responses Mildew.

Язык: Английский

Процитировано

0

Identifying Critical Regulators in the Viral Stress Response of Wheat (Triticum aestivum L.) Using Large-Scale Transcriptomics Data DOI Creative Commons
Amir Ghaffar Shahriari, Imre Majláth,

Massume Aliakbari

и другие.

Agronomy, Год журнала: 2023, Номер 13(10), С. 2610 - 2610

Опубликована: Окт. 13, 2023

Wheat (Triticum aestivum L.) cultivation has been globally restricted by many plant viruses such as the streak mosaic virus (WSMV), Barley stripe (BSMV), and Brome (BMV). Herein, transcriptome of wheat was in silico analyzed under mono- (WSMV, BSMV, or BMV), bi- (BMV&BSMV, BMV&WSMV, BSMV&WSMV), tripartite BMV) infections using RNA-seq technique. Total numbers 1616/270, 5243/690 5589/2183 differentially expressed genes (DEGs) were up/down-regulated during bipartite infection BMV&BSMV, BMV&WSMV BSMV&WSMV, respectively, while resulted up/down-regulation 6110/2424 DEGs. The NAC bHLH most commonly presented transcription factor (TF) families WSMV, BMV, BSMV infection, C2H2, bHLH, TF involved BSMV&WSMV infections, respectively. RLK-Pelle_DLSV protein kinase (PK) family all patterns. Promoter analysis showed that motifs gene expression, CUL4 RING ubiquitin ligase complex, stress response, brassinosteroid energy-related pathways significantly induced plants infections. expression network a defense-related gene, i.e., allene oxide synthase (AOS) serves crucial hub

Язык: Английский

Процитировано

0

Unraveling growth molecular mechanisms inPinus taedawith GWAS, machine learning, and gene coexpression networks DOI Creative Commons
Alexandre Hild Aono, Stephanie Karenina Bajay, Felipe Roberto Francisco

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Дек. 10, 2022

Abstract Pinus taeda (loblolly pine [LP]) is a long-lived tree species and one of the most economically significant forest species. Among growth traits, volume widely considered trait in improvement programs. However, deciphering genetic variants responsible for variations conifers, such as LP, particularly challenging due to vast size intricate complexity genomes. We present comprehensive analysis focusing on markers associated with stem variation, elucidate molecular mechanisms governing high-performance phenotypes. used population 1,692 individuals phenotyped genotyped these using sequence capture probes. To conduct genome-wide associations, we utilized both association study (GWAS) machine learning (ML) approaches. The identified were found be linked genes assembled from three distinct transcriptomes. These subsequently construct gene coexpression networks, through topological evaluations, key potential regulatory roles within configurations. Using set 31,589 SNPs, defined 7 GWAS-associated SNPs 128 ML-associated markers, all which correlated multiple involved diverse biological functions. Gene revealed group 270 potentially regulation material. Key directly implicated response stress identified, inferences about their impact development elucidated. Our not only offers insights into but also elucidates subset characterized by unique features. findings significantly advance our understanding factors influencing reveal candidate future functional studies, contribute broader comprehension architecture underlying traits LP.

Язык: Английский

Процитировано

0