A Comparative Transcriptomic Study Reveals Temporal and Genotype-Specific Defense Responses to Botrytis cinerea in Grapevine

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 124 - 124

Опубликована: Фев. 7, 2025

Grapevine (Vitis vinifera L.), a globally significant crop, is highly susceptible to Botrytis cinerea, the causative agent of gray mold disease. This study investigates transcriptomic responses B. cinerea in tolerant and grapevine genotypes using RNA sequencing (RNA-seq). Differentially expressed genes (DEGs) were identified at three time points (T1, T2, T3), highlighting both genotype-independent genotype-specific responses. Early-stage infection (T1) revealed rapid robust activation defense pathways genotypes, though genotype showed enhanced modulation metabolic processes by prioritizing secondary metabolism stress adaptation over growth. In contrast, exhibited less coordinated reprogramming, with delayed or weaker key mechanisms. Gene Ontology KEGG analyses critical pathways, including phenylpropanoid biosynthesis-like lignin metabolism, MAPK signaling, as well candidate such WRKY transcription factors enzymes involved cell wall fortification antifungal compound biosynthesis. Genotype-specific emphasized flexibility determinant resistance, exhibiting superior resource allocation pathways. These findings provide insights into molecular basis resistance offering potential targets for breeding genetic engineering enhance resilience reduce fungicide dependency.

Язык: Английский

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Grape Berry Morphology in Semi-Arid Climate of Tekirdağ: Evaluating the Effects of Environmental Factors and Stress Applications

Black Sea Journal of Agriculture, Год журнала: 2024, Номер 7(2), С. 144 - 156

Опубликована: Янв. 24, 2024

The growth and development of grapes are influenced by various biotic abiotic stresses. presence Vitis vinifera L. on Earth is threatened the increase in stresses due to global warming. On other hand, grape quality and, consequently, berry characteristics can also be negatively affected these stress factors. hypothesis this experiment determine effects applied five days before harvest berries live grapevines under field conditions. For purpose, for two years (2016 2017), Cabernet-Sauvignon Merlot varieties grafted onto SO4 rootstock at Te-Ha Corp. vineyard were used. In late pre-harvest period (five harvest), seven applications, including control, implemented. application methods included impact (1 minute with a plastic hammer 08:00 19:00), leaf removal (removing all leaves), injury (injuring leaves hitting stick), UV-C vibration Botrytis cinerea Pers ex. Fr (once). measurements features performed as follows, order: width-length (mm), bery volume (cm3), skin area (cm2/grain), area/berry flesh ratio (cm2/cm3), fresh-dry weight (g), 100 fresh density (g/cm3), % dry weight. As result, it was observed that treatments did not affect years, especially second year. Therefore, Shock action, UV-C, Vibration, Leaf injury, removal, improving found objectionable.

Язык: Английский

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Effect of abiotic stresses on primary / secondary metabolites and resveratrol in cv. Cabernet-Sauvignon

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Год журнала: 2025, Номер 28(1), С. 70 - 82

Опубликована: Янв. 30, 2025

The research was located at latitude 41° 01’ 11.15” N and longitude 27° 40’ 18.00” E, an altitude of 60 m above sea level, with 15-year-old Cabernet-Sauvignon/110R vines over two years. vineyard has a planting distance 2.6×0.9 m, the are trellised to double cordon Royat. In vineyard, 4 abiotic stress applications (Shock Action, Leaf Injury, UV-C) including Control were applied twice day (morning evening) for 5 days during 3 different phenological stages (Veraison, Veraison-Harvest, Harvest). Injury performed once by striking leaves rod. Shock Action carried out using plastic hammer, UV-C 1 minute. As result, it determined that did not cause significant differences in primary metabolites such as Total Soluble Solids (23.69°Brix) Acidity (7.32 g L-1) but had increasing effect on secondary (total tannin, anthocyanin, TPC, resveratrol). Additionally, found they enhancing TPI. terms resveratrol, effects (0.35 mg kg-1) (0.27 noted be higher than other Control).

Язык: Английский

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Integration of RNAseq and WGCNA to study phenotypic plasticity in wine grape cultivars grown in central-southern Italy

Acta Horticulturae, Год журнала: 2025, Номер 1418, С. 173 - 184

Опубликована: Фев. 1, 2025

Язык: Английский

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Metabolic and physiological analysis reveals distinct salinity tipping point in Vitis vinifera cv. Syrah to enter a stress response mode

Plant Stress, Год журнала: 2025, Номер 16, С. 100864 - 100864

Опубликована: Апрель 23, 2025

Язык: Английский

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Enhancing Abiotic Stress Resilience in Mediterranean Woody Perennial Fruit Crops: Genetic, Epigenetic, and Microbial Molecular Perspectives in the Face of Climate Change

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3160 - 3160

Опубликована: Март 29, 2025

Enhanced abiotic stresses such as increased drought, elevated temperatures, salinity, and extreme weather phenomena severely affect major crops in the Mediterranean area, a ‘hot spot’ of climate change. Plants have evolved mechanisms to face stressful conditions adapt environmental pressures. Intricate molecular processes involving genetic epigenetic factors plant–microbe interactions been implicated response tolerance stress. Deciphering whereby plants perceive respond stress is crucial for developing strategies counteract challenges. Progress determining genes, complex gene networks, biochemical pathways, well plant–microbiota crosstalk, involved has achieved through application tools diverse resources. This knowledge could be particularly useful accelerating plant improvement generating resilient varieties, especially concerning woody perennial crops, where classical breeding lengthy labor-intensive process. Similarly, understanding provide insights into innovative approaches facing conditions. In this review, we comprehensive overview discuss recent findings genetic, epigenetic, microbial aspects shaping responses, context enhancing resilience important fruit crops.

Язык: Английский

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Transcriptomic and Metabolomic Insights into ABA-Related Genes in Cerasus humilis under Drought Stress

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7635 - 7635

Опубликована: Июль 11, 2024

Cerasus humilis, a small shrub of the genus within Rosaceae family, is native to China and renowned for its highly nutritious medicinal fruits, robust root system, remarkable drought resistance. This study primarily employed association transcriptome metabolome analyses assess changes in abscisic acid (ABA) levels identify key regulatory genes C. humilis subjected varying degrees stress. Notably, we observed distinct alterations transcription factors across different intensities. Specifically, our data indicated noteworthy shifts GATA, MYB, MYC, WRKY, C2H2, bHLH factor families. Furthermore, combined transcriptomic metabolomic investigations demonstrated significant enrichment metabolic pathways, such as ‘Carbon metabolism’, ‘Biosynthesis amino acids’, cofactors’, ‘Phenylpropanoid biosynthesis’, ‘Starch sucrose ‘Plant hormone signal transduction’ under moderate (Mod) or severe (Sev) conditions. A total 11 candidate involved ABA biosynthesis signaling pathways were identified. The down-regulated included secoisolariciresinol dehydrogenase-like PYL2. Conversely, including FAD-dependent urate hydroxylase-like, cytochrome P450 97B2, carotenoid cleavage dioxygenase 4 (CCD4), SnRK2.2, ABI 5-like protein 5, PP2C 51, SnRK2.3, up-regulated Mod Sev lays genetic foundation enhance tolerance provides resources plant engineering breeding efforts.

Язык: Английский

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Study of red vine phenotypic plasticity across central-southern Italy sites: an integrated analysis of the transcriptome and weather indices through WGCNA

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Ноя. 11, 2024

The grapevine (

Язык: Английский

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Advances in fruit coloring research in grapevine: an overview

Plant Growth Regulation, Год журнала: 2023, Номер 103(1), С. 51 - 63

Опубликована: Дек. 14, 2023

Язык: Английский

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Effects of different rootstocks on the growth and yield characteristics of Papazkarası (Vitis vinifera L.)

International Journal of Agriculture Environment and Food Sciences, Год журнала: 2024, Номер unknown, С. 591 - 601

Опубликована: Сен. 11, 2024

Rootstocks, which are becoming increasingly important in viticulture, influence the growth, yield, and grape quality of grafted variety. Therefore, understanding resistance characteristics rootstocks to phylloxera, nematodes, environmental conditions, abiotic biotic stresses is crucial. Selecting a rootstock that appropriate for region where vineyard will be established optimizes quality. The aim this research determine performance cv. Papazkarası on different rootstocks. For purpose, an experiment was at Irem Çamlica Viticulture Winery Co. Kırklareli province. Ten-year-old vines onto 1103P, 110R, 420A were used as plant material. To growth vines, parameters such shoot elongation rate (cm/week), length changings (cm), pruning wood weight (PW) (kg/vine), vigor (g), puissance, number buds per square meter (number), balanced (number/vine), vegetative (VG), Ravaz Index (RI), Partridge (PI), yield (kg/vine) examined. Additionally, cluster characteristics, width volume gappy spaceless clusters (cm³) measured. results indicated 1103P had lowest RI, PI, VG values; moderate values PW, vigor, shoots, berries cluster, length; highest weight, clusters. 110R found more compared other rootstocks, with PI; average VG; length, VG, clusters; value one-year-old cane weight. In conclusion, based outlined, selection can made from these according cultivation purposes, but should also investigated.

Язык: Английский

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