Preharvest and Postharvest γ-Aminobutyric Acid Treatment Enhance Quality and Shelf Life in Strawberry (Fragaria × ananassa) Fruits

Journal of Plant Growth Regulation, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Язык: Английский

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Integrate analysis of metabolome and transcriptome of three Fragaria × ananassa cultivars to stablish the non-volatile compounds of strawberry flavor

LWT, Год журнала: 2024, Номер 198, С. 116043 - 116043

Опубликована: Апрель 1, 2024

The flavor attribute of Fragaria × ananassa Duch. would decide consumers' selection preferred strawberry commercial cultivars. roles volatile compounds in aroma have been well-studied while knowledge regarding the non-volatile is limited. Some which are involved consumers preference reported, contributions for remain ambiguous. This study explored influences essential by comparing biosynthesis pathways cultivars exhibiting distinct characteristics. widely targeted metabolome generated global profiles three including 521 known compounds. differential accumulation analysis and precise quantification soluble sugars, organic acids, polyphenols free amino acids (FAAs) predicted fructose, citric acid, kaempferol pelargonin-3-glucoside were key sweetness, acidity astringency strawberries. Further, conjoint metabolites 824 differentially expressed genes revealed that flavonoid biosynthesis, phenylpropanoid metabolism citrate cycle decided content difference Within these pathways, several structure as potential regulators flavonoids umami-taste FAAs. These findings be helpful identification responsible flavor.

Язык: Английский

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Effect of edible coating incorporating sodium carboxymethyl cellulose/cellulose nanofibers and self-produced mandarin oil on strawberries

Food Packaging and Shelf Life, Год журнала: 2023, Номер 40, С. 101197 - 101197

Опубликована: Окт. 5, 2023

Edible coatings were prepared using a mixture of 1.0 % (w/v) sodium carboxymethyl cellulose (CMC), 0.5 nanofibers (CNF), and varying concentrations mandarin oil (MO) at 0.05, 0.1, 0.2, 0.3, or 0.4 (w/v). The research investigated the physicochemical characteristics antifungal activities films on Botrytis cinerea (B. cinerea). CMC/CNF/MO used to preserve strawberries (Amaou). changes in quality activity assessed during storage 5 °C 85 ± relative humidity (RH). Results showed major components MO include β-pinene, limonene, methyl palmitate. addition into indicated significant effect properties coating solutions as well film shown b* roughness values increased strongly. contained also represented marked fruit weight loss against B. strain both vitro vivo. concentration 0.05 illustrated controlling strawberries.

Язык: Английский

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Exploration of a European-centered strawberry diversity panel provides markers and candidate genes for the control of fruit quality traits

Horticulture Research, Год журнала: 2024, Номер unknown

Опубликована: Май 14, 2024

Abstract Fruit quality traits are major breeding targets in cultivated strawberry (Fragaria × ananassa). Taking into account the requirements of both growers and consumers when selecting high cultivars is a real challenge. Here, we used diversity panel enriched with unique European accessions 50K FanaSNP array to highlight evolution over past 160 years, investigate molecular basis 12 fruit by Genome-Wide Association Studies (GWAS), provide genetic markers for breeding. Results show that considerable improvements key including weight, firmness, composition appearance occurred simultaneously American cultivars. Despite our panel, observed drop nucleotide certain chromosomal regions, revealing impact selection. GWAS identified 71 associations 11 and, while validating known (firmness, sugar), highlighted predominance new QTL, demonstrating value using untapped resources. Three six selective sweeps detected related glossiness or skin resistance, two little-studied important attractiveness potentially, postharvest shelf-life. Moreover, QTL glossiness, resistance susceptibility bruising found within low region chromosome 3D. Stringent search candidate genes underlying uncovered strong candidates color, sugar acid composition, resistance. Overall, study provides potential avenue extending shelf-life without compromising flavor color as well needed achieve this goal.

Язык: Английский

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The accumulation and regulation of malate in fruit cell

Fruit Research, Год журнала: 2024, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Fruit acidity is an important component of flavor quality in fleshy fruit. The accumulation malate, the dominant organic acid formation most mature fruit, highly regulated by metabolism and transportation during fruit development. While, knowledge still limited about mechanism acidification, as well major genes substances. In present paper, research advance on relevance between malate associated with transportation, transcriptional regulation was reviewed. Furthermore, positive future could provide theoretical reference for optimization genetic improvement.

Язык: Английский

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ClaPEPCK4: target gene for breeding innovative watermelon germplasm with low malic acid and high sweetness

GM crops & food, Год журнала: 2025, Номер 16(1), С. 156 - 170

Опубликована: Янв. 14, 2025

Malic acid markedly affects watermelon flavor. Reducing the malic content can significantly increase sweetness of watermelon. An effective solution strategy is to reduce through molecular breeding technology. In this study, we measured TSS and pH six varieties at four growth nodes. The was very low 10 DAP accumulated rapidly 18, 26, 34 DAP. Three phosphoenolpyruvate carboxykinase (PEPCK) genes were identified analyzed. ClaPEPCK4 expression inversely proportional malate variations in fruits. transgenic plants, overexpressing gene, decreased. knockout two SNP mutations one base deletion occurred with leaves increasing considerably PEPCK enzyme activity reduced half wild-type. It interesting that gene triggered closure leaf stomata under dark conditions which indicated its involvement stomatal movement. conclusion, study provides a target for creating innovative new high-sweetness varieties.

Язык: Английский

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Genome-wide analysis and functional validation reveal the role of late embryogenesis abundant genes in strawberry (Fragaria × ananassa) fruit ripening

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Март 1, 2024

Abstract Background Late embryogenesis abundant (LEA) proteins play important roles in plant growth and development, as well stresses responsiveness. Nowadays, it has been found that LEAs also have function fruit ripening. However, the comprehensive analysis on a genome-wide basis of LEA family remains limited, role ripening not fully explored yet, especially strawberry, an economic ideal material for studying Results In this study, total 266 putative were identified characterized strawberry genome. Subcellular localization prediction indicated they mostly localized chloroplast, cytoplasm nucleus. Duplication events detection revealed whole genome duplication or segmental was main driver expansion strawberry. The phylogenetic suggested FaLEAs classified into eight groups, among which, LEA2 largest subgroup with 179 members, followed by LEA3, dehydrin (DHN), LEA4 SMP (seed maturation protein). LEA1 DHN groups speculated to dominant development ripening, according their larger proportion members detected differentially expressed genes during such process. Notably, expression FaLEA167 belonging group altered maturation, inhibited overexpression negative regulators (a cytosolic/plastid glyceraldehyde-3-phosphate dehydrogenase, FaGAPC2 cytosolic pyruvate kinase, FaPKc2.2 ). Subsequently, significantly increased percentage at green stage, while reduced full red proportion. consistent, anthocyanins content skin color variable reflecting range from greenness redness ( a* value) reduced. Whereas, apparently up-regulated citric acid, soluble protein malondialdehyde content, but had no obvious effects solids, sugar, flavonoids, phenolics antioxidant capacity. Conclusions These findings only provided basic information FaLEA further functional research, involvement negatively regulating giving new insights understanding functions.

Язык: Английский

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Metabolomic and Transcriptomic Analysis Revealed the Maturation Mechanism of White-Fleshed Strawberry

Agronomy, Год журнала: 2024, Номер 14(12), С. 2860 - 2860

Опубликована: Ноя. 30, 2024

Strawberry (Fragaria ananassa) is a widely grown horticultural crop, which exists in red, yellow, and white varieties. In recent years, the white-fleshed strawberry variety gaining more attention from consumers for its unique taste appearance, but comprehensive understanding of molecular processes governing ripening remains undisclosed. this study, based on joint analysis physiology, metabolome, transcriptome, we screened identified key metabolites that were highly correlated to maturation (cv. ‘snow white’, SW short) fruits. contrast red-fleshed strawberries, fruits exhibited three main stages during maturation, accompanied by increases total soluble solid sugar declines anthocyanin acid. Metabolomic 832 differential accumulated (DAMs) at secondary level LC-MS/MS, further investigations suggested increase sucrose, citric acid, epicatechin levels potentially play role process Furthermore, abscisic acid methyl jasmonate recognized as primary phytohormones involved production these metabolites. The enrichment RNA-Seq data revealed expressed genes (DEGs) primarily attributed pathways ‘Starch sucrose metabolism’ ‘Plant hormone signal transduction’ undetected ‘Flavonoid biosynthesis’ late stage. Moreover, de novo biosynthesis pathway, WGCNA, Pearson correlation indicated direct relationship between FaSPS1, FaSPP1, FaSPP2 with FaPEPC1, FaV-PPase2, FaV-PPase3 Fa4CL2, Fa4CL3, FaANR1 anthocyanin. Further co-expression MYBs, bHLHs, NACs, WRKYs structural mentioned. Overall, our findings uncovered mechanism regulating strawberry, providing valuable insights enhancing flavor through gene-editing technique.

Язык: Английский

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Integrated transcriptomic and metabolomic analyses revealed the role of SlMYC2 in tomato (Solanum lycopersicum L.) fruit development and ripening

Scientia Horticulturae, Год журнала: 2023, Номер 325, С. 112691 - 112691

Опубликована: Ноя. 18, 2023

Язык: Английский

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Integrative analysis of metabolome and transcriptome reveals new insights into major quality formation and regulation in two strawberry cultivars with different fruit flavor

Vegetable Research, Год журнала: 2024, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Soluble sugars, organic acids, and vitamin C (AsA) are the major metabolites contributing to strawberry fruit quality. To date, metabolomics genetic information for above still limited. In this study, we integrated metabolome transcriptome in two cultivars with remarkably different The significant difference of fructose, sucrose, malic acid contents illustrated flavor discrepancy cultivars, addition, recycling pathway showed vital role AsA accumulation. Conjoint analysis implied key metabolizing enzymes related soluble acids AsA, respectively, further construction co-expression regulatory networks identified transcription factors structural genes. Specifically, a high potential was suggested that accumulation sugars were synchronously regulated by MYB, bHLH, NAC, AP2 bZIP family members during ripening. This work provided new insights into regulation core fruits, gene resources promising improvement quality molecular breeding.

Язык: Английский

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