International Heart Journal,
Год журнала:
2024,
Номер
65(3), С. 517 - 527
Опубликована: Май 30, 2024
Myocardial
infarction/reperfusion
(I/R)
injury
significantly
impacts
the
health
of
older
individuals.
We
confirmed
that
level
lncRNA
Peg13
was
downregulated
in
I/R
injury.
However,
detailed
function
myocardial
has
not
yet
been
explored.To
detect
Peg13,
vivo
model
constructed.
RT-qPCR
employed
to
investigate
RNA
levels,
and
Western
blotting
performed
assess
levels
endoplasmic
reticulum
stress
apoptosis-associated
proteins.
EdU
staining
cell
proliferation.I/R
therapy
dramatically
produced
injury,
increased
infarct
area,
decreased
amount
tissues
mice.
In
addition,
hypoxia/reoxygenation
(H/R)
notably
induced
apoptosis
promoted
(ER)
HL-1
cells,
while
overexpression
reversed
these
phenomena.
Additionally,
may
increase
Sirt1
through
binding
miR-34a.
Upregulation
H/R-induced
ER
via
regulation
miR-34a/Sirt1
axis.LncRNA
reduces
mediation
axis.
Hence,
our
research
might
shed
new
lights
on
developing
strategies
for
treatment
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 28, 2025
Introduction
Earlier
sowing
is
a
promising
strategy
of
ensuring
sufficiently
high
maize
yields
in
the
face
negative
environmental
factors
caused
by
climate
change.
However,
it
leads
to
low
temperature
exposure
plants
during
emergence,
warranting
better
understanding
their
response
and
acclimation
suboptimal
temperatures.
Materials
Methods
To
achieve
this
goal,
whole
transcriptome
sequencing
was
performed
on
two
inbred
lines
–
tolerant/susceptible
temperatures,
at
5-day-old
seedling
stage.
Sampling
after
6h
24h
treatment
(10/8°C).
The
data
filtered,
mapped,
identified
mRNAs,
lncRNAs,
circRNAs
were
quantified.
Expression
patterns
RNAs,
as
well
interactions
between
them,
analyzed
reveal
ones
important
for
low-temperature
response.
Results
Discussion
Genes
involved
different
steps
photosynthesis
downregulated
both
genotypes:
psa,
psb,
lhc
,
cab
genes
photosystem
I
II
functioning,
rca,
prk,
rbcx1
necessary
Calvin
cycle.
difference
tolerance
genotypes
appeared
arise
from
ability
mitigate
damage
photoinhibition:
ctpa2,
grx,
elip,
UF3GT
showed
higher
expression
tolerant
genotype.
Certain
lncRNAs
also
targeted
these
genes,
creating
an
interaction
network
induced
(XLOC_016169-
rca
;
XLOC_002167-XLOC_006091-
elip2
).
These
findings
shed
light
potential
mechanisms
emergence
lay
groundwork
subsequent
analyses
across
diverse
developmental
stages.
As
such,
offers
valuable
guidance
future
research
directions
molecular
breeding
maize.
Journal of Experimental Botany,
Год журнала:
2023,
Номер
75(6), С. 1741 - 1753
Опубликована: Авг. 30, 2023
Abstract
Tobacco
(Nicotiana
tabacum
L.)
is
a
widely
cultivated
crop
of
the
genus
Nicotiana.
Due
to
highly
addictive
nature
tobacco
products,
smoking
remains
leading
cause
preventable
death
and
disease.
There
therefore
critical
need
develop
varieties
with
reduced
or
non-addictive
nicotine
levels.
Nicotine
related
pyridine
alkaloids
biosynthesized
in
roots
plants
are
transported
leaves,
where
they
stored
vacuoles
as
defense
against
predators.
Jasmonate,
defense-related
plant
hormone,
plays
crucial
signaling
role
activating
transcriptional
regulators
that
coordinate
expression
downstream
metabolic
transport
genes
involved
production.
In
recent
years,
substantial
progress
has
been
made
molecular
genomics
research,
revealing
many
regulatory
biosynthesis.
These
advances
have
enabled
us
low
ultra-low
levels
through
various
methodologies,
such
mutational
breeding,
genetic
engineering,
genome
editing.
We
review
on
manipulation
production
tobacco,
which
serves
an
excellent
example
engineering
profound
social
implications.
ABSTRACT
The
challenge
of
feeding
the
world's
growing
population
is
impaired
by
declining
arable
land,
water
quality
and
erratic
weather
patterns
due
to
climate
change.
Abiotic
stresses
such
as
drought,
heat,
salinity
cold
disrupt
plant
growth,
reducing
crop
yields
quality.
Modern
biotechnological
tools
including
high‐throughput
sequencing
bioinformatics
have
enabled
characterization
stress
responses
through
advanced
“omics”
technologies.
Genomics,
transcriptomics,
proteomics,
metabolomics
epigenomics
describe
molecular
mechanisms
underlying
tolerance.
Integrating
multi‐omics
approaches
provides
a
deeper
understanding
these
mechanisms,
addressing
limitations
single‐omics
studies.
combination
data
(genomics,
proteomics
metabolomics)
identifies
important
biomarkers,
regulatory
networks
genetic
targets
that
enhance
resilience.
This
information
regarding
plants
crucial
for
genome‐assisted
breeding
(GAB)
improve
traits
development
climate‐resilient
crops
withstand
environmental
challenges.
Therefore,
researchers
use
pipelines
productive
crops,
tolerance,
solving
global
food
security
challenges
caused
change
stressors.
review
discusses
role
omics
technologies
in
describing
explores
how
this
applied
resilience
productivity,
which
leads
improved
crops.
application
combining
develop
next‐generation
are
capable
thriving
under
adverse
conditions,
ensuring
reliable
safe
supply
future
conditions.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3486 - 3486
Опубликована: Апрель 8, 2025
The
brown
planthopper
(BPH)
is
one
of
the
major
rice
pests
causing
significant
damage
to
production
worldwide,
due
its
high
reproductive
capacity
and
strong
migratory
ability.
A
series
BPH-resistant
varieties
have
been
developed,
but
durability
effective
resistance
constrained
by
evolution
BPH
virulence,
requiring
in-depth
insights
into
mechanisms.
In
this
paper,
we
used
strand-specific
lncRNA-seq
characterize
lncRNA
regulatory
mechanisms
on
response
infestation.
Overall,
4321
lncRNAs
were
identified,
60
which
significantly
upregulated
in
infestation,
specifically
differing
between
populations
with
variable
virulence.
Differential
expression
analysis
qRT-PCR
validation
showed
that
these
are
involved
regulation
several
defense
pathways,
including
jasmonic
acid
signaling
flavonoid
biosynthesis,
their
distinct
roles
resistant
susceptible
varieties.
Notably,
like
LNC_002533
found
be
negatively
correlated
suggesting
a
potential
role
modulating
responses.
contrast,
LNC_001986
LNC_000397
positively
genes
glutathione
metabolism,
may
associated
enhanced
resistance.
These
findings
highlight
critical
functions
rice-BPH
interactions
provide
molecular
framework
for
improving
through
targeted
genetic
engineering.
This
study
contributes
functional
genomics
elucidating
lncRNA-mediated
offers
promising
avenues
developing
durable
pest-resistant
