Genetic Loci of Plant Pathogenic Dickeya solani IPO 2222 Expressed in Contact with Weed-Host Bittersweet Nightshade (Solanum dulcamara L.) Plants DOI Open Access
Robert Czajkowski, Dorota M. Krzyżanowska, Daryna Sokolova

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(5), С. 2794 - 2794

Опубликована: Фев. 28, 2024

Dickeya solani, belonging to the Soft Rot Pectobacteriaceae, are aggressive necrotrophs, exhibiting both a wide geographic distribution and host range that includes many angiosperm orders, dicot monocot plants, cultivated under all climatic conditions. Little is known about infection strategies D. solani employs infect hosts other than potato (Solanum tuberosum L.). Our earlier study identified Tn5 mutants induced exclusively by presence of weed S. dulcamara. The current assessed identity virulence contribution selected genes mutated insertions These encode proteins with functions linked polyketide antibiotics polysaccharide synthesis, membrane transport, stress response, sugar amino acid metabolism. Eight these genes, encoding UvrY (GacA), tRNA guanosine transglycosylase Tgt, LPS-related WbeA, capsular biosynthesis protein VpsM, DltB alanine export protein, glycosyltransferase, putative transcription regulator YheO/PAS domain-containing hypothetical were required for on dulcamara plants. implications interaction host, dulcamara, discussed.

Язык: Английский

Agricultural Pest Management: The Role of Microorganisms in Biopesticides and Soil Bioremediation DOI Creative Commons
Alane Beatriz Vermelho, Jean Vinícius Moreira, Ingrid Teixeira Akamine

и другие.

Plants, Год журнала: 2024, Номер 13(19), С. 2762 - 2762

Опубликована: Окт. 1, 2024

Pesticide use in crops is a severe problem some countries. Each country has its legislation for use, but they differ the degree of tolerance these broadly toxic products. Several synthetic pesticides can cause air, soil, and water pollution, contaminating human food chain other living beings. In addition, them accumulate environment an indeterminate amount time. The agriculture sector must guarantee healthy with sustainable production using environmentally friendly methods. this context, biological biopesticides from microbes plants are growing green solution segment. pests attack worldwide, including weeds, insects, nematodes, microorganisms such as fungi, bacteria, viruses, causing diseases economic losses. bioproducts microorganisms, microbial (MBPs) or alone, practice due to intense research world. Mainly, baculoviruses have been used sources biomolecules secondary metabolites biopesticide use. Different methods, direct soil application, spraying techniques endotherapy, seed treatment, used. Adjuvants like surfactants, protective agents, carriers improve system different formulations. tool bioremediation environment. This review summarizes topics, focusing on origin.

Язык: Английский

Процитировано

12

Shedding light on bacteria–host interactions with the aid of TnSeq approaches DOI Creative Commons
Marta Torres,

Sarah Paszti,

Leo Eberl

и другие.

mBio, Год журнала: 2024, Номер 15(6)

Опубликована: Май 9, 2024

Bacteria are highly adaptable and grow in diverse niches, where they often interact with eukaryotic organisms. These interactions different hosts span the entire spectrum from symbiosis to pathogenicity thus determine lifestyle of bacterium. Knowledge genetic determinants involved animal plant host colonization by pathogenic mutualistic bacteria is not only crucial discover new drug targets for disease management but also developing novel biostimulant strategies. In last decades, significant progress genome-wide high-throughput technologies such as transposon insertion sequencing has led identification pathways that enable efficient colonization. However, extent which similar genes play a role this process yet unclear. This review highlights commonalities specificities bacterial important bacteria-host interaction.

Язык: Английский

Процитировано

7

Combined Analysis of Transcriptomes and Metabolomes Reveals Key Genes and Substances That Affect the Formation of a Multi-Species Biofilm by Nine Gut Bacteria DOI Creative Commons
Ting Zhang, Zhangming Pei, Hongchao Wang

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 234 - 234

Опубликована: Янв. 22, 2025

Biofilms are one of the ways microorganisms exist in natural environments. In recent years, research has gradually shifted its focus to exploring complexity and interactions multi-species biofilms. A study showed that nine gut bacteria can form a biofilm on wheat fibers (M9 biofilm). However, previous did not clarify reasons why M9 exhibited better formation ability than mono-species this study, gene expression levels metabolic accumulation biofilms each individual bacterium were analyzed using transcriptomes metabolomes. The differentially expressed genes (DEGs) there 740 common DEGs existed all groups, they could regulate five pathways related bacterial motility, cellular communication, signal transduction. metabolome results revealed many peptides/amino acids derivatives produced biofilm. Furthermore, purine metabolism was significantly enhanced L-arginine, l-serine, guanosine, hypoxanthine accumulated metabolites (DAMs). combined analysis metabolomes 26 highly correlated with four DAMs, involved amino purines. These indicate by modulating transduction, This provides insights into microbial

Язык: Английский

Процитировано

0

Gene expression analyses on Dickeya solani strains of diverse virulence levels unveil important pathogenicity factors for this species DOI Creative Commons

Weronika Babińska-Wensierska,

Agata Motyka‐Pomagruk, Alessio Mengoni

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 25, 2025

Dickeya solani causes soft rot and blackleg mainly on potato crops. High pathogenicity of this species results from efficient production plant cell wall-degrading enzymes, especially pectate lyases, potent root colonization, fast vascular movement. Despite genomic homogeneity, variations in virulence-related phenotypes suggest differences the gene expression patterns between diverse strains. Therefore, methylomes transcriptomes two strains (virulent IFB0099 low virulent IFB0223), differing tissue maceration capacity virulence factors production, have been studied. Methylation analysis revealed no significant differences. However, transcriptomes, studied under both non-induced induced by polygalacturonic acid conditions (in order to mimic stages infection process), unveiled higher lyases (pelD, pelE, pelL), pectin esterase (pemA), proteases (prtE, prtD) Vfm-associated quorum-sensing genes (vfmC, vfmD, vfmE) strain compared IFB0223. Additionally, related secretion system II (T2SS) (gspJ, nipE) displayed induction IFB0099. Furthermore, showed more elevated involved flagella formation, which coincides with enhanced motility To sum up, differential important for D. indicated candidate genes, might be crucial species.

Язык: Английский

Процитировано

0

Surviving the Potato Stems: Differences in Genes Required for Fitness by Dickeya dadantii and Dickeya dianthicola DOI
Juliana González‐Tobón, Tyler C. Helmann, Paul Stodghill

и другие.

Phytopathology, Год журнала: 2024, Номер 114(5), С. 1106 - 1117

Опубликована: Янв. 3, 2024

Bacteria belonging to the genus

Язык: Английский

Процитировано

1

Genetic Loci of Plant Pathogenic Dickeya solani IPO 2222 Expressed in Contact with Weed-Host Bittersweet Nightshade (Solanum dulcamara L.) Plants DOI Open Access
Robert Czajkowski, Dorota M. Krzyżanowska, Daryna Sokolova

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(5), С. 2794 - 2794

Опубликована: Фев. 28, 2024

Dickeya solani, belonging to the Soft Rot Pectobacteriaceae, are aggressive necrotrophs, exhibiting both a wide geographic distribution and host range that includes many angiosperm orders, dicot monocot plants, cultivated under all climatic conditions. Little is known about infection strategies D. solani employs infect hosts other than potato (Solanum tuberosum L.). Our earlier study identified Tn5 mutants induced exclusively by presence of weed S. dulcamara. The current assessed identity virulence contribution selected genes mutated insertions These encode proteins with functions linked polyketide antibiotics polysaccharide synthesis, membrane transport, stress response, sugar amino acid metabolism. Eight these genes, encoding UvrY (GacA), tRNA guanosine transglycosylase Tgt, LPS-related WbeA, capsular biosynthesis protein VpsM, DltB alanine export protein, glycosyltransferase, putative transcription regulator YheO/PAS domain-containing hypothetical were required for on dulcamara plants. implications interaction host, dulcamara, discussed.

Язык: Английский

Процитировано

1