Foods,
Год журнала:
2024,
Номер
13(12), С. 1884 - 1884
Опубликована: Июнь 15, 2024
Exploring
the
sugar
and
amino
acid
content
variability
influence
of
thermal
processing
on
these
in
soybeans
can
help
optimize
their
utilization
animal
feed.
This
study
examined
209
samples
harvested
2020
55
2021
from
across
U.S.
to
assess
variability.
Harvest
regions
included
East
Corn
Belt,
West
Mid-South,
Coast,
Southeast
In
addition
contents,
protein,
oil,
seed
size
were
also
analyzed.
Samples
evaluated
for
contents
before
after
autoclaving
seeds
at
105–110
°C
15
min.
For
2020,
sucrose
(4.45
g
100
g−1)
stachyose
(1.34
most
prevalent
sugars.
2021,
L-arginine
(9.82
g−1),
leucine
(5.29
glutamate
(4.90
acids.
Heat
treatment
resulted
an
8.47%,
20.88%,
11.18%,
1.46%
median
loss
free
lysine,
sucrose,
glucose,
fructose.
study’s
insights
into
heat-induced
changes
nutritional
composition
provide
a
reference
improving
soybean
quality
assessment
optimizing
its
use
feed
formulations
Background:
The
quality
of
soybeans
is
reflected
in
the
seed
coat
color,
which
indicates
soybean
and
commercial
value.
Researchers
have
identified
genes
related
to
color
various
plants.
However,
research
on
regulation
rare.
Methods:
In
this
study,
four
lines
coats
with
different
colors
(medium
yellow
14,
black,
green,
brown)
were
selected
from
F2:5
population,
Beinong
108
as
female
parent
green
bean
male
parent,
dynamic
changes
anthocyanins
stained
4-dimethylaminocinnamaldehyde
(DMACA)
during
grain
maturation
process
(20
days
drum
harvest).
Through
RNA-seq
colored
at
30
50
after
seeding,
we
can
further
understand
key
pathways
gene
modules
between
colors.
Results:
DMACA
revealed
that
black
produce
first
deepest
staining.
Clustering
principal
component
analysis
(PCA)
data
divided
eight
samples
into
two
groups,
resulting
16,456
DEGs,
including
5359
TFs.
GO
KEGG
enrichment
analyses
flavonoid
biosynthesis,
starch
sucrose
metabolism,
carotenoid
circadian
rhythm
significantly
enriched.
We
also
conducted
statistical
expression
pattern
differentially
expressed
transcription
factors.
Based
weighted
coexpression
network
(WGCNA),
seven
specific
connectivity
functional
annotation
within
calculated,
21
candidate
identified,
six
factor
(TF)
three
pathway
genes.
Conclusions:
These
findings
provide
a
theoretical
basis
for
an
in-depth
understanding
molecular
mechanisms
underlying
differences
new
genetic
resources.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 28, 2024
Seed
size/weight
plays
an
important
role
in
determining
crop
yield,
yet
only
few
genes
controlling
seed
size
have
been
characterized
soybean.
Here,
we
perform
a
genome-wide
association
study
and
identify
major
quantitative
trait
locus
(QTL),
named
GmSW17
(Seed
Width
17),
on
chromosome
17
that
determine
soybean
width/weight
natural
population.
encodes
ubiquitin-specific
protease,
ortholog
to
UBP22,
belonging
the
protease
(USPs/UBPs)
family.
Further
functional
investigations
reveal
interacts
with
GmSGF11
GmENY2
form
deubiquitinase
(DUB)
module,
which
influences
H2Bub
levels
negatively
regulates
expression
of
GmDP-E2F-1,
thereby
inhibiting
G1-to-S
transition.
Population
analysis
demonstrates
undergo
artificial
selection
during
domestication
but
has
not
fixed
modern
breeding.
In
summary,
our
identifies
predominant
gene
related
weight,
providing
potential
advantages
for
high-yield
breeding
Plants,
Год журнала:
2024,
Номер
13(7), С. 940 - 940
Опубликована: Март 24, 2024
Red
crown
rot
(RCR)
disease
caused
by
Calonectria
ilicicola
negatively
impacts
soybean
yield
and
quality.
Unfortunately,
the
knowledge
of
genetic
architecture
RCR
resistance
in
soybeans
is
limited.
In
this
study,
299
diverse
accessions
were
used
to
explore
their
diversity
RCR,
mine
for
candidate
genes
via
emergence
rate
(ER),
survival
(SR),
severity
(DS)
a
multi-locus
random-SNP-effect
mixed
linear
model
GWAS.
All
had
brown
necrotic
lesions
on
primary
root,
with
five
genotypes
identified
as
resistant.
Nine
single-nucleotide
polymorphism
(SNP)
markers
detected
underlie
response
(ER,
SR,
DS).
Two
SNPs
colocalized
at
least
two
traits
form
haplotype
block
which
possessed
nine
genes.
Based
annotation
qRT-PCR,
three
genes,
namely
Glyma.08G074600,
Glyma.08G074700,
Glyma.12G043600,
are
suggested
modulate
RCR.
The
findings
from
study
could
serve
foundation
breeding
RCR-tolerant
varieties,
be
validated
deepen
our
understanding
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 14, 2025
As
complex
quantitative
traits,
soybean
seed
oil
and
protein
contents
are
governed
by
dynamic
proteome
networks
that
remain
largely
unknown.
Here,
we
investigated
the
changes
of
during
maturation
across
two
varieties
with
contrasting
content.
Through
optimizing
detectability
low-abundance
proteins
utilizing
library-free
data-independent
acquisition
(directDIA)
mass
spectrometry,
unprecedentedly
identified
7414
3975
groups
(PGs),
substantially
expanding
proteome.
Among
PGs,
1391
differentially
accumulated
between
varieties.
By
comparing
abundance
PGs
varieties,
core
periphery
seeds
revealed
variations
in
content
primarily
attributed
to
peripheral
proteome,
which
significantly
fluctuated
developmental
stages.
Our
work
presents
a
proteomic
atlas
underlying
variation
provides
insight
into
mechanisms
regulating
soybean.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 949 - 949
Опубликована: Янв. 23, 2025
This
study
presents
an
advanced
approach
for
the
comprehensive
analysis
of
low-abundance
proteins
in
soybean
seeds,
addressing
challenges
posed
by
high-abundance
storage
proteins.
We
compared
effectiveness
Data-Dependent
Acquisition
(DDA),
Data-Independent
(DIA),
and
BoxCar
mass
spectrometry
techniques
to
identify
two
types
seeds:
High-Oil
High-Protein
seeds.
The
results
indicate
that
DIA
method,
particularly
methods,
significantly
improve
detection
DDA,
offering
deeper
insights
into
seed
biology.
Specifically,
BoxCar-based
revealed
distinct
proteomic
differences
between
highlighting
more
active
metabolic
processes
Additionally,
several
key
were
identified
annotated
as
uniquely
expressed
either
or
These
findings
emphasize
importance
techniques,
such
BoxCar,
deepening
our
understanding
biology
supporting
breeding
strategies
nutritional
qualities.
The Open Agriculture Journal,
Год журнала:
2025,
Номер
19(1)
Опубликована: Фев. 14, 2025
Introduction/Objective
Soybean
is
a
major
source
of
various
nutrients.
Increasing
demand
for
soybeans
has
created
considerable
impetus
exploring
the
nutritional
quality
soybeans.
We
aimed
to
collect
soybean
varieties
rich
in
Materials
and
Methods
Metabolite
analysis
was
carried
out
seed
compositions,
including
protein,
phenolics,
flavonoids,
along
with
gene
expression
protein
phenolic
metabolism-related
enzymes
10
accessions
collected
from
different
geographical
regions.
Results
Total
content
ranged
29.7%
35.7%,
depending
on
germplasm
accessions.
Among
them,
Vang
Ha
Giang
(VHG)
exhibited
relatively
high
content,
while
Cuc
Vo
Nhai
(CVN)
had
comparatively
low
content.
Further
compounds
indicated
that
were
higher
cultivars
Dau
Tuong
Den
(DTD)
CVN,
total
37.7
µg
g
-1
flavonoid
2.1
mg
.
These
results
reinforced
by
levels
candidate
genes
β-conglycinin
(7S)
glycinin
(11S)
involving
storage,
phenylalanine
ammonia-lyase
1
(
GmPAL1
)
chalcone
synthase
8
GmCHS8)
related
synthesis,
which
showed
similar
correlation.
revealed
correlated
weight
but
not
color,
even
though
significant
variations
found
among
genotypes,
affected
coat
color.
Furthermore,
negative
correlation
flavonoids
demonstrated
intricate
relationships
components.
Conclusion
Protein
alteration
seeds
subject
major-effect-genotypes
landrace
breeding
cultivar
selection,
genotype
variants
are
relevant
Our
study
provides
components
offers
insight
into
quality.