Triacontanol Reverses Abscisic Acid Effects on Stomatal Regulation in Solanum lycopersicum L. under Drought Stress Conditions

Horticulturae, Год журнала: 2024, Номер 10(9), С. 985 - 985

Опубликована: Сен. 18, 2024

When applied under abiotic stress conditions, triacontanol (TRIA) is effective in regulating the physicochemical processes plants through mechanisms of defence such as abscisic acid (ABA) signalling. However, TRIA’s role relation to ABA and stomatal opening unclear. Therefore, objective this study was evaluate effects TRIA their combinations on different variables related regulation Solanum lycopersicum, which subjected drought stress, leaf epidermis. The negative responses triggered by were reversed treated with TRIA. increased conductance photosynthetic activity early hours, it determined that produced larger stomata than did other treatments. Moreover, chloroplasts significantly smaller more numerous those control, could improve CO2 diffusion efficiency may be photosynthesis. Finally, abaxial epidermis tests reaffirmed inhibitory opening. These results confirm important various ABA, regulation.

Язык: Английский

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Effect of humidity on postmortem changes in rats

Journal of Veterinary Science, Год журнала: 2024, Номер 25(3)

Опубликована: Янв. 1, 2024

In veterinary forensic science, accurately determining the postmortem interval (PMI) is crucial for identifying causes of animal deaths. Autolysis, a significant process, influences PMI estimation, but its relationship with humidity not well understood.

Язык: Английский

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Physico-chemical features and functional relevance of tomato rhomboid proteases

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 272, С. 132681 - 132681

Опубликована: Май 26, 2024

Язык: Английский

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Integrating Gene Expression Analysis and Ecophysiological Responses to Water Deficit in Leaves of Tomato Plants

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 5, 2024

Abstract Soil water deficit (WD) is one of the most important abiotic stresses affecting plant survival and crop yield. Despite its economic relevance, many gaps remain in our understanding how crops respond to WD, especially concerning synergistic coordination molecular ecophysiological adaptations delaying damage mortality. In this study, we investigated gene expression imposed by a progressive WD combined it with measurements pointing key thresholds leaves tomato plants. We uncovered transcriptomic changes mature at four stages defined physiological markers relating different intensities: partial stomatal closure, complete after leaf wilting, beginning embolism development veins. By identifying transcription factors (TFs) across these progressively worsening stages, timing impact ABA-(in)dependent regulatory pathways during WD. addition, compared transcriptome young developing versus explored mechanisms that may explain higher tolerance dehydration younger leaves. correlating precise measurements, dataset will serve as framework for future studies comparing responses specific intensities. Highlight Integrated analyses identify underlying

Язык: Английский

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Water Relations and Physiological Response to Water Deficit of ‘Hass’ Avocado Grafted on Two Rootstocks Tolerant to R. necatrix

Agronomy, Год журнала: 2024, Номер 14(9), С. 1959 - 1959

Опубликована: Авг. 29, 2024

Avocado (Persea americana Mill.) cultivation has spread to many countries from the tropics Mediterranean region, where avocado crops commonly face water shortages and diseases, such as white root rot (WRR) caused by Rosellinia necatrix. The use of drought- WRR-tolerant rootstocks represents a potential solution these constraints. In this research, relations morpho-physiological response ‘Hass’ grafted on two selections R. necatrix-tolerant (BG48 BG181) were evaluated under well-watered (WW) at soil-water-availability conditions (WS, ~50% ~25% field capacity). Under WW, scion was markedly affected rootstock, with BG48 displaying water-spender behavior, showing higher consumption (~20%), plant transpiration rates (~30%; Eplant) leaf photosynthetic AN) than BG181, which exhibited water-saving strategy based upon trade-off between leaf-biomass allocation tight stomatal control transpiration. This did not reduce biomass, BG181 plants being more efficient. WS, drought-avoidance behavior distinct underlying mechanisms, but increases in mass area (~18–12%; LMA), decreases Eplant (~50–65%), hydraulic conductance (~44–86%; Kh) (~48–73%; Ψw) observed both rootstocks, aligned stress severity. After rewatering, fully recovered, suggesting some ability withstand stress, enabling variety adapt region-specific

Язык: Английский

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Integrating gene expression analysis and ecophysiological responses to water deficit in leaves of tomato plants

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 22, 2024

Soil water deficit (WD) significantly impacts plant survival and crop yields. Many gaps remain in our understanding of the synergistic coordination between molecular ecophysiological responses delaying substantial drought-induced effects on growth. To investigate this synergism tomato leaves, we combined molecular, ecophysiological, anatomical methods to examine gene expression patterns physio-anatomical characteristics during a progressing WD experiment. Four sampling points were selected for transcriptomic analysis based key leaves: 4 5 days after (d-WD), corresponding 10% 90% decrease leaf stomatal conductance; 8 d-WD, wilting point; 10 when air embolism blocks 12% xylem transport. At upregulated genes mostly linked ABA-independent responses, with larger-scale ABA-dependent occurring at d-WD. observed an upregulation heat shock transcription factors, two later (10 d-WD), found strong oxidative stress factors. Finally, that young leaves present stronger dehydration tolerance than mature same drought intensity level, presumably because upregulate related increased callose deposition resulting limiting loss phloem, cell rigidity by modifying wall structures. This dataset will serve as framework future studies aim obtain more holistic response level.

Язык: Английский

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Multiomics plasticity in seed traits of pan-genome wheat cultivars

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 21, 2024

Abstract The molecular basis of cultivar-level variations in polyploid wheat that enables environmental adaptation while maintaining yield and quality remains poorly understood. We conducted a detailed phenotypic assessment multiomics analysis nine pan-genome cultivars grown under control drought conditions. aimed to investigate the subgenome-level variations, cultivar differences biochemical mechanisms affecting plant fitness moderate stress. Intrinsic water use efficiency, grain yield, protein content differed among cultivars, supporting plasticity stress responses. Biased proteome metabolome abundance changes response during vegetative stage indicate different strategies for utilization homeologous isoforms assigned A, B, D subgenomes. Drought effects were detected at level, but significant observed central carbon pathway metabolites micronutrient profiles. subgenomic localization seed storage proteins highlight nutrient reservoir accumulation emphasizes enhanced role S-rich prolamins response. Subgenomic define phenotypes by producing molecules accumulate enable underlying trade-offs between yield- or quality-related traits. These can be used select crops with increased resistance without compromising yield.

Язык: Английский

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Exogenous abscisic acid treatment regulates protein secretion in sorghum cell suspension cultures

Plant Signaling & Behavior, Год журнала: 2023, Номер 18(1)

Опубликована: Дек. 15, 2023

Drought stress adversely affects plant growth, often leading to total crop failure. Upon sensing soil water deficits, plants switch on biosynthesis of abscisic acid (ABA), a hormone for drought adaptation. Here, we used exogenous ABA application dark-grown sorghum cell suspension cultures as an experimental system understand how drought-tolerant responds ABA. We evaluated intracellular and secreted proteins using isobaric tags relative absolute quantification. While the abundance only ~ 7% (46 proteins) changed in response ABA, ~32% (82 identified this study were responsive. This shows that extracellular matrix is disproportionately targeted suggests it plays vital role adaptation drought. Extracellular responsive predominantly defense/detoxification wall-modifying enzymes. confirmed exposed activate genes encoding same vitro culture with Our results suggest activates defense wall remodeling systems during response. could underpin success stress.

Язык: Английский

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Editorial: Plant proteolytic enzymes: contributions and challenges to improve food availability against climate change effects

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 9, 2024

Editorial: Plant proteolytic enzymes: contributions and challenges to improve food availability against climate change effects

Язык: Английский

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Identifying Candidate Genes for Sugar Accumulation in Sugarcane Cultivars: From a Syntenic Genomic Region to a Gene Coexpression Network

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 12, 2024

Abstract Elucidating the intricacies of sugarcane genome is essential for breeding superior cultivars. This economically important crop originates from hybridizations highly polyploid Saccharum species. However, large size (10 Gb), high polyploidy, and aneuploidy pose significant challenges to complete sequencing, assembly, annotation. One successful strategy identifying candidate genes linked agronomic traits, particularly those associated with sugar accumulation, leverages synteny potential collinearity related In this study, we explored between sorghum sugarcane. Genes a Brix QTL were used screen bacterial artificial chromosome (BAC) libraries two Brazilian varieties (IACSP93-3046 SP80-3280). The entire region was successfully recovered, confirming Manual annotation identified 51 in hybrid that subsequently confirmed be present spontaneum . To identify study employed multifaceted approach, including retrieving genomic interest, performing gene-by-gene analysis, analyzing RNA-seq data internodes officinarum S. accessions, constructing coexpression network examine expression patterns within studied their neighbors, finally differentially expressed (DEGs). comprehensive approach led discovery three potentially involved accumulation: an ethylene-responsive transcription factor (ERF), ABA 8’-hydroxylase, prolyl oligopeptidase (POP). These findings could valuable additional other agricultural traits directly targeting further work molecular breeding.

Язык: Английский

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