Genomic engineering in peanut

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 159 - 175

Опубликована: Янв. 1, 2024

Язык: Английский

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Effects of Ethylene Inhibitors on the Long-Term Maintenance of the Embryogenic Callus of Vitis vinifera L.

Horticulturae, Год журнала: 2024, Номер 10(10), С. 1038 - 1038

Опубликована: Сен. 29, 2024

Vitis vinifera is an important fruit crop which mainly consumed fresh or used for the production of wine. Genetic improvement programs through New Genomic Techniques (NGTs) aim to develop grapevine varieties resistant biotic and abiotic stresses enhancing nutraceutical properties. In order apply NGTs, maintaining embryogenic calluses from flower tissues critical. Optimizing culture conditions—pH, gelling agents, temperature, light, growth regulators, gas composition—is essential inducing efficient responses tailored each genotype/explant. Ethylene, a pivotal gaseous plant hormone, significantly influences tissue by affecting organogenesis embryogenesis processes in several plants. Modulating ethylene levels shows promise improving vitality. This study evaluates effects silver thiosulfate (STS) salicylic acid (SA) on callus growth, specifically investigating their roles competence. STS, particularly at 40 µM 60 concentrations, effectively preserved competence Italia Red Globe calluses, while high SA concentrations showed varied occasionally adverse effects. At same time, STS markedly suppressed non-embryogenic recalcitrant variety Italia, potentially increasing ratio between development.

Язык: Английский

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Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 11902 - 11902

Опубликована: Ноя. 6, 2024

Selectable marker genes are useful for recognizing which cells have integrated specific sequences in their genome after genetic transformation processes. They especially important fruit trees to individuate putatively genetically modified events, because most of the protocols used engineer these species often unsuccessful or with low efficiency. Traditional selectable genes, mainly bacterial origin, confer antibiotics/herbicides-resistance metabolic advantages transformed cells. Genes that allow visual recognition engineered tissues without using any selective agent, such as morphogenic regulators and reporter also selection tools vitro identify regenerated lines. As final step, plants should be tested field conditions, where no longer necessary, strongly unpopular commercial development new products. Thus, different approaches, based on use site-specific recombinases and/or editing nucleases, being now recover marker-free crops. This review describes comments suitable interest, particularly tree engineering. Lastly, a spotlight highlights biosafety aspects related exploited

Язык: Английский

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Development and Applications of Somatic Embryogenesis in Grapevine (Vitis spp.)

Plants, Год журнала: 2024, Номер 13(22), С. 3131 - 3131

Опубликована: Ноя. 7, 2024

Somatic embryogenesis (SE) provides alternative methodologies for the propagation of grapevine (

Язык: Английский

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