Gene editing and GWAS for digital imaging analysis of wheat grain weight, size and shape are inevitable to enhance the yield
Cereal Research Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 15, 2025
Язык: Английский
Plant membrane transporters function under abiotic stresses: a review
Planta,
Год журнала:
2024,
Номер
260(6)
Опубликована: Окт. 24, 2024
Язык: Английский
The TaGW2‐TaSPL14 module regulates the trade‐off between tiller number and grain weight in wheat
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(9), С. 1953 - 1965
Опубликована: Июнь 24, 2024
ABSTRACT
IDEAL
PLANT
ARCHITECTURE1
(IPA1)
is
a
pivotal
gene
controlling
plant
architecture
and
grain
yield.
However,
little
known
about
the
effects
of
Triticum
aestivum
SQUAMOSA
PROMOTER‐BINDING‐LIKE
14
(TaSPL14),
an
IPA1
ortholog
in
wheat,
on
balancing
yield
traits
its
regulatory
mechanism
wheat
(
T.
L.).
Here,
we
determined
that
GRAIN
WIDTH2
(TaGW2)‐TaSPL14
module
influences
balance
between
tiller
number
weight
wheat.
Overexpression
TaSPL14
resulted
reduced
increased
weight,
whereas
knockout
had
opposite
effect,
indicating
negatively
regulates
tillering
while
positively
regulating
weight.
We
further
identified
TaGW2
as
novel
interacting
protein
confirmed
ability
to
mediate
ubiquitination
degradation
TaSPL14.
Based
our
genetic
evidence,
acts
positive
regulator
number,
addition
role
negative
which
Moreover,
combinations
TaSPL14‐7A
TaGW2‐6A
haplotypes
exhibit
significantly
additive
breeding.
Our
findings
provide
insight
into
how
TaGW2‐TaSPL14
trade‐off
potential
application
improving
Язык: Английский
Fine-mapping and candidate gene identification for QPtn.sau-4B showing potential in increasing productive tiller number and yield in wheat
The Crop Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 1, 2025
Язык: Английский
The regulation of grain weight in wheat
Seed Biology,
Год журнала:
2023,
Номер
2(1), С. 0 - 0
Опубликована: Янв. 1, 2023
Wheat
(Triticum
aestivum
L.,
AABBDD)
is
one
of
the
world's
most
extensively
cultivated
crops,
furnishing
vital
nutrients
and
energy
for
human
consumption.
seeds
are
primary
sustenance
source.
Given
mounting
global
population
dwindling
arable
land,
enhancing
wheat
grain
yield
remains
a
concern
mankind.
A
pivotal
agronomic
trait
influencing
weight,
which
predominantly
contingent
on
seed
size
endosperm
components
regulated
by
complex
precise
molecular
networks.
Endogenous
factors,
such
as
transcriptional
post-translational
regulators,
exert
influence
over
development.
Notably,
starch
main
storage
component
endosperm,
synthesis-related
genes
an
important
effect
weight.
Prior
reviews
traits
have
mostly
focused
regulation
size,
contents
almost
entirely
written
based
regulatory
network
rice
size.
Although
many
mechanisms
various
similar
in
wheat,
there
lots
differences
due
to
its
vast
intricate
genome.
An
all-encompassing
panorama
weight
has
not
yet
been
comprehensive.
This
review
summarizes
catalog
reported
genes,
discusses
emerging
mechanisms,
delves
into
networks
foster
more
holistic
understanding
Язык: Английский
Grain size control in wheat: toward a molecular understanding
Seed Biology,
Год журнала:
2024,
Номер
3(1), С. 0 - 0
Опубликована: Янв. 1, 2024
Grain
size
is
a
major
determinate
of
bread
wheat
(Triticum
aestivum)
yield,
which
has
broad
impact
on
worldwide
food
security.
Not
surprisingly,
grain
underwent
extensive
artificial
selection
during
domestication
and
breeding.
Recent
advances
in
molecular
genetics
genomics
have
facilitated
the
elucidation
basis
underlying
size.
determination
cumulative
result
source
strength,
photoassimilate
remobilization,
sink
strength.
Here,
we
systematically
review
recent
progress
cloning
mechanisms
genes
that
regulate
following
to
flow.
In
addition,
discuss
possible
strategies
for
overcoming
trade-off
between
number,
as
well
synergetic
improvement
yield
quality.
Язык: Английский
Coordination of miR319–TaPCF8 with TaSPL14 orchestrates auxin signaling and biosynthesis to regulate plant height in common wheat
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(11), С. 2362 - 2378
Опубликована: Авг. 7, 2024
Wheat
culms,
comprising
four
to
six
internodes,
are
critically
involved
in
determining
plant
height
and
lodging
resistance,
essential
factors
for
field
performance
regional
adaptability.
This
study
revealed
the
regulatory
function
of
miR319
common
wheat
height.
Repression
tae-miR319
through
short
tandem
target
mimics
(STTM)
caused
an
increased
height,
while
overexpression
(OE)
had
opposite
effect.
Overexpressing
a
miR319-resistant
gene
TaPCF8
(rTaPCF8),
acted
as
transcription
repressor
downstream
genes
TaIAAs,
which
interact
physically
with
TaSPL14.
The
significant
differences
indole-3-acetic
acid
(IAA)
contents
indicate
involvement
auxin
pathway
miR319-mediated
regulation.
Finally,
we
identified
two
haplotypes
global
collections.
TaPCF8-5A-Hap2,
per
association
evolution
examinations,
was
subjected
strong
substantial
selection
throughout
breeding.
haplotype,
associated
shorter
aligns
breeding
requirements.
Consequently,
high-yield
breeding,
proposed
potential
molecular
marker
marker-assisted
(MAS).
Our
findings
offer
fresh
perspectives
into
mechanisms
that
underlie
miR319-TaPCF8
module's
regulation
by
orchestrating
signaling
biosynthesis
wheat.
Язык: Английский
Characterization of tae-miR156(s) and their response to abiotic stress in wheat (Triticum aestivum L.)
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 4, 2024
The
microRNA156
(miR156)
has
been
widely
studied
in
plants,
however,
the
characterization
of
miR156
family
genes
wheat
and
their
expression
patterns
under
abiotic
stress
are
not
completely
clear.
In
this
study,
a
total
20
members,
referred
to
as
tae-miR156a
tae-miR156t,
were
identified
with
loci
mapped
various
chromosomes.
These
members
divided
into
five
subgroups:
miR156a/b/c/d/e/f,
miR156g/h/i,
miR156j/k,
miR156l/m/n/o/p/q,
miR156r/s/t.
They
highly
conserved
during
evolution.
prediction
cis-elements
tae-MIR156(s)
promoter
region
revealed
that
had
diverse
cis-acting
elements;
these,
15
6
found
be
drought-responsive
elements
cold-responsive
elements,
respectively.
And
target
tae-miR156(s)
mainly
SPL
transcription
factor
genes.
Expression
analysis
based
on
quantitative
real-time
polymerase
chain
reaction
(qRT‒PCR)
showed
miR156(s)
have
different
levels
tissues,
subgroups'
response
varied.
Among
them,
miR156g/h/i
strongly
induced
root
cold
heat
stress,
miR156a/b/c/d/e/f
significantly
increased
roots
after
drought
whereas
miR156r/s/t
inhibited
leaves
salt
stress.
findings
imply
involved
wheat,
they
provide
new
fundamental
knowledge
for
further
function
its
regulatory
mechanism
Язык: Английский
Genome wide association and haplotype analyses for the crease depth trait in bread wheat (Triticum aestivum L.)
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Июль 3, 2023
Wheat
grain
has
a
complex
structure
that
includes
crease
on
one
side,
and
tissues
within
the
region
play
an
important
role
in
nutrient
transportation
during
wheat
development.
However,
genetic
architecture
of
is
still
unclear.
In
this
study,
413
global
accessions
were
resequenced
method
was
developed
for
evaluating
phenotypic
data
depth
(CD).
The
CD
values
exhibited
continuous
considerable
large
variation
population,
broad-sense
heritability
84.09%.
found
to
be
positively
correlated
with
grain-related
traits
negatively
quality-related
traits.
Analysis
differentiation
between
landraces
cultivars
revealed
simultaneously
improved
breeding
improvement.
Moreover,
2,150.8-Mb
segments
identified
fall
selective
sweeps
cultivars;
they
contained
some
known
functional
genes
quality-
Genome-wide
association
study
(GWAS)
performed
using
around
10
million
SNPs
generated
by
genome
resequencing
551
significant
18
QTLs
detected
significantly
associated
CD.
Combined
cluster
analysis
gene
expression,
haplotype
analysis,
annotated
information
candidate
genes,
two
promising
TraesCS3D02G197700
TraesCS5A02G292900
potentially
regulate
To
best
our
knowledge,
first
provide
basis
CD,
loci
may
ultimately
assist
programs.
Язык: Английский
Integration of mRNA and miRNA analysis reveals the molecular mechanisms of sugar beet (Beta vulgaris L.) response to salt stress
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Дек. 12, 2023
Abstract
The
continuous
increase
of
saline-alkali
areas
worldwide
has
led
to
the
emergence
conditions,
which
are
primary
abiotic
stress
or
hindering
growth
plants.
Beet
is
among
main
sources
sugar,
and
its
yield
sugar
content
notably
affected
by
stress.
Despite
beet
being
known
as
a
salt-tolerant
crop,
there
few
studies
on
mechanisms
underlying
salt
tolerance,
previous
have
mainly
delineated
crop’s
response
induced
NaCl.
Recently,
advancements
in
miRNA-mRNA
network
analysis
an
increased
understanding
how
plants,
including
beet,
respond
In
this
study,
seedlings
variety
"N98122"
were
grown
laboratory
using
hydroponics
culture
exposed
at
40
days
growth.
According
phenotypic
adaptation
seedlings'
leaves
from
state
turgidity
wilting
then
back
before
after
exposure,
18
different
time
points
selected
collect
samples
for
analysis.
Subsequently,
based
data
real-time
quantitative
PCR
(qRT-PCR)
salt-responsive
genes,
collected
0,
2.5,
7.5,
16
h
subjected
further
with
experimental
materials.
Next,
mRNA-seq
identification
8455
differentially
expressed
mRNAs
(DEMs)
under
exposure
addition,
miRNA-seq
investigation
retrieved
3558
miRNAs
stress,
encompassing
887
belonging
783
families
2,671
novel
miRNAs.
With
integrated
network,
57
miRNA-target
gene
pairs
obtained,
consisting
55
DEMIs
DEMs.
Afterwards,
we
determined
pivotal
involvement
aldh2b7
,
thic
δ-oat
genes
effect
identified
novel-m035-5p
novel-m0365-5p
regulating
aldh
miRNA
novel-m0979-3p
gene.
findings
mRNA
expression
validated
qRT-PCR.
Язык: Английский