Nanopore
sequencing
enables
detection
of
DNA
methylation
at
the
same
time
as
identification
canonical
sequence.
A
recent
study
validated
low-pass
nanopore
to
accurately
estimate
global
levels
in
vertebrates
with
coverage
low
0.01x.
We
investigated
applicability
this
approach
plants
by
testing
three
plant
species
and
analysed
effect
technical
biological
parameters
on
precision
accuracy.
Our
results
indicate
that
higher
(0.1x)
is
required
achieve
accuracy
assessing
comparable
vertebrates.
Shorter
read
length
a
closer
sequence
match
between
sample
reference
genome
improved
measurement
Application
method
Vitis
vinifera
showed
consistent
across
different
leaf
sizes,
preservation
extraction
methods,
whereas
varieties
tissue
types
did
exhibit
differences.
Similarly,
distinct
patterns
were
observed
genomic
features.
findings
suggest
suitability
low-cost
screening
tool
for
validation
experimental
parameters,
developmental
courses,
assess
status
modification
contexts
level
whole
or
abundant
features
such
transposable
elements.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3160 - 3160
Опубликована: Март 29, 2025
Enhanced
abiotic
stresses
such
as
increased
drought,
elevated
temperatures,
salinity,
and
extreme
weather
phenomena
severely
affect
major
crops
in
the
Mediterranean
area,
a
‘hot
spot’
of
climate
change.
Plants
have
evolved
mechanisms
to
face
stressful
conditions
adapt
environmental
pressures.
Intricate
molecular
processes
involving
genetic
epigenetic
factors
plant–microbe
interactions
been
implicated
response
tolerance
stress.
Deciphering
whereby
plants
perceive
respond
stress
is
crucial
for
developing
strategies
counteract
challenges.
Progress
determining
genes,
complex
gene
networks,
biochemical
pathways,
well
plant–microbiota
crosstalk,
involved
has
achieved
through
application
tools
diverse
resources.
This
knowledge
could
be
particularly
useful
accelerating
plant
improvement
generating
resilient
varieties,
especially
concerning
woody
perennial
crops,
where
classical
breeding
lengthy
labor-intensive
process.
Similarly,
understanding
provide
insights
into
innovative
approaches
facing
conditions.
In
this
review,
we
comprehensive
overview
discuss
recent
findings
genetic,
epigenetic,
microbial
aspects
shaping
responses,
context
enhancing
resilience
important
fruit
crops.
Plants,
Год журнала:
2024,
Номер
13(10), С. 1400 - 1400
Опубликована: Май 17, 2024
As
global
arid
conditions
worsen
and
groundwater
resources
diminish,
drought
stress
has
emerged
as
a
critical
impediment
to
plant
growth
development
globally,
notably
causing
declines
in
crop
yields
even
the
extinction
of
certain
cultivated
species.
Numerous
studies
on
resistance
have
demonstrated
that
DNA
methylation
dynamically
interacts
with
responses
by
modulating
gene
expression
developmental
processes.
However,
precise
mechanisms
underlying
these
interactions
remain
elusive.
This
article
consolidates
latest
research
role
across
various
species,
focusing
methods
detection,
pattern
alteration
(including
de
novo
methylation,
maintenance
demethylation),
overall
conditions.
While
many
observed
significant
shifts
genome-wide
or
promoter
levels
drought-stressed
plants,
identification
specific
genes
pathways
involved
remains
limited.
review
aims
furnish
reference
for
detailed
into
through
epigenetic
approaches,
striving
identify
regulated
signaling
pathways,
their
molecular
action.
