Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 24, 2024
Double-stranded
RNA
(dsRNA)
has
emerged
as
key
player
in
gene
silencing
for
the
past
two
decades.
Tailor-made
dsRNA
is
now
recognized
a
versatile
raw
material,
suitable
wide
range
of
applications
biopesticide
formulations,
including
insect
control
to
pesticide
resistance
management.
The
mechanism
interference
(RNAi)
acts
at
messenger
(mRNA)
level,
utilizing
sequence-dependent
approach
that
makes
it
unique
term
effectiveness
and
specificity
compared
conventional
agrochemicals.
Two
primary
categories
small
RNAs,
known
short
interfering
RNAs
(siRNAs)
microRNAs
(miRNAs),
function
both
somatic
germline
lineages
broad
eukaryotic
species
regulate
endogenous
genes
defend
genome
from
invasive
nucleic
acids.
Furthermore,
application
RNAi
crop
protection
can
be
achieved
by
employing
plant-incorporated
protectants
through
plant
transformation,
but
also
non-transformative
strategies
such
use
formulations
sprayable
direct
agents,
factor
repressors
or
developmental
disruptors.
This
review
explores
agricultural
RNAi,
delving
into
its
successes
pest-insect
considering
broader
potential
managing
pathogens,
nematodes,
pests.
Additionally,
tool
addressing
pesticide-resistant
weeds
insects
reviewed,
along
with
an
evaluation
production
costs
environmental
implications.
ABSTRACT
The
partial
nucleotide
sequence
of
the
coat
protein
(CP)
gene
Ethiopian
isolates
chickpea
chlorotic
stunt
virus
(CpCSV,
genus
Polerovirus
),
beet
western
yellows
(BWYV,
and
soybean
dwarf
(SbDV,
Luteovirus
)
was
determined
from
lentil
plants
showing
yellowing,
stunting,
reddening
symptoms.
Comparative
analysis
CpCSV
obtained
five
showed
94.9%–100%
91.9%–98.7%
identity
with
each
other
reference
isolates,
respectively.
One
isolate
(MZ043728)
a
close
relationship
serotype
II
while
remaining
nine
were
closely
related
to
belonging
I.
Sequence
identities
three
BWYV
varied
93.3%
100%
one
them
(MZ043727)
displayed
previously
reported
(LStV,
).
MZ043725
MZ043726
appear
be
identical
other,
whereas
identified
LStV
isolate.
SbDV
had
lower
GenBank
their
phylogenetic
that
they
are
clustered
separately
rest
indicating
most
divergent.
This
result
generates
essential
information
for
further
research
on
legume
viruses
in
Ethiopia.
In
addition,
detailed
study
should
conducted
future
understand
prevalence
determine
potential
yield
losses
associated
Abstract
The
flowering
time
(FTi)
plays
a
critical
role
in
the
reproductive
success
and
yield
of
various
crop
species
by
directly
impacting
both
quality
quantity
grain
yield.
Achieving
optimal
FTi
is
crucial
for
maximizing
ensuring
overall
agricultural
productivity.
While
genetic
factors
undoubtedly
influence
FTi,
photoperiodism
vernalization
are
recognized
as
key
contributors
to
complex
physiological
processes
governing
plants.
Identifying
candidate
genes
pathways
associated
with
essential
developing
genomic
interventions
plant
breeding
enhance
adaptability
diverse
environmental
conditions.
This
review
highlights
intricate
nature
regulatory
mechanisms
emphasizes
vital
importance
precisely
regulating
ensure
success.
Special
attention
given
genes,
pathways,
geared
toward
promoting
early
flowering,
particularly
under
challenging
conditions
such
drought,
heat,
cold
stress
well
other
abiotic
stresses
that
occur
during
stage
major
field
crops.
Moreover,
this
explores
significant
progress
achieved
omics
technologies,
offering
valuable
insights
tools
deciphering
FTi.
In
summary,
aims
provide
comprehensive
understanding
particular
focus
on
their
bolstering
yields
adverse
safeguard
food
security.
Frontiers in Genome Editing,
Год журнала:
2023,
Номер
5
Опубликована: Сен. 21, 2023
Plant
genome
editing,
a
recently
discovered
method
for
targeted
mutagenesis,
has
emerged
as
promising
tool
crop
improvement
and
gene
function
research.
Many
genome-edited
plants,
such
rice,
wheat,
tomato,
have
over
the
last
decade.
As
preliminary
steps
in
procedure
editing
involve
genetic
transformation,
amenability
to
depends
on
efficiency
of
engineering.
Hence,
there
are
numerous
reports
aforementioned
crops
because
they
transformed
with
relative
ease.
Legume
rich
protein
and,
thus,
favored
source
plant
proteins
human
diet
most
countries.
However,
legume
cultivation
often
succumbs
various
biotic/abiotic
threats,
thereby
leading
high
yield
loss.
Furthermore,
certain
legumes
like
peanuts
possess
allergens,
these
need
be
eliminated
deprive
many
people
from
gaining
benefits
crops.
Further
variations
limited
legumes.
Genome
potential
offer
solutions
not
only
combat
stress
but
also
generate
desirable
knock-outs
variants.
excluding
soybean,
alfalfa,
Outlook on Agriculture,
Год журнала:
2023,
Номер
52(3), С. 294 - 302
Опубликована: Сен. 1, 2023
Legume-based
cropping
systems
are
regaining
interest
due
to
the
environmental
services
and
variety
of
food
feed
uses
they
provide,
this
having
special
in
agroecological
systems.
There
many
legume
crops
that
can
be
adopted
but
focused
breeding
is
needed
better
meet
specific
requirements
each
system,
especially
situation
changing
climate
often
stressful
environments.
Standard
methods
remain
valid,
from
classical
selection,
genomic
assisted-breeding,
profiting
modern
biotechnological
approaches
which
rapidly
developing
for
most
crops.
Rather
than
focusing
on
tools,
emphasis
targets,
might
different
those
high
input
agriculture
such
as
improved
response
symbiosis,
nutrient
water
use
efficiency
tolerance
biotic
abiotic
stresses
becoming
priorities.
Breeding
these
traits
requires
infusion
genetic
diversity
landraces
or
wild
relatives
by
pre-breeding.
Prospects
constraints
discussed.
Horticulturae,
Год журнала:
2024,
Номер
10(3), С. 262 - 262
Опубликована: Март 10, 2024
Analysis
of
the
viromes
three
symptomatic
Fabaceae
plants,
i.e.,
red
clover
(Trifolium
pratense
L.),
pea
(Pisum
sativum
and
common
bean
(Phaseolus
vulgaris
using
high-throughput
sequencing
revealed
complex
infections
enabled
acquisition
complete
genomes
a
potyvirus,
yellow
mosaic
virus
(BYMV).
Based
on
phylogenetic
analysis,
Slovak
BYMV
isolates
belong
to
two
distinct
molecular
groups,
VI
(isolate
FA40)
XI
(isolates
DAT,
PS2).
Five
commercial
genotypes
(Alderman,
Ambrosia,
Gloriosa,
Herkules,
Senator)
were
successfully
infected
with
BYMV-PS2
inoculum
displayed
similar
systemic
chlorotic
mottling
symptoms.
Relative
comparison
optical
density
values
semi-quantitative
DAS-ELISA
significant
differences
among
titers
in
one
(Ambrosia)
when
upper
fully
developed
leaves
tested.
Immunoblot
analysis
systemically
Alderman
plants
showed
rather
uneven
accumulation
different
plant
parts.
The
lowest
was
repeatedly
detected
roots,
while
highest
part
stem.
International Journal of Environment and Climate Change,
Год журнала:
2023,
Номер
13(9), С. 1461 - 1474
Опубликована: Июль 19, 2023
Yellow
mosaic
virus
(YMV)
is
a
destructive
viral
pathogen
that
affects
green
gram
(Vigna
radiata)
crops,
leading
to
significant
yield
losses
and
economic
repercussions.
This
review
paper
provides
comprehensive
analysis
of
the
impacts
YMV
on
cultivation,
explores
current
management
strategies
employed
combat
disease,
discusses
future
perspectives
for
effective
control
prevention.
primarily
transmitted
through
whiteflies
infects
plants
at
various
stages
growth,
causing
severe
symptoms
such
as
leaf
yellowing,
stunted
patterns.
These
ultimately
lead
reduced
crop
quality.
The
poses
major
threat
production
globally,
demanding
urgent
attention
practices.
Various
approaches
have
been
mitigate
impact
YMV.
Cultural
practices,
including
proper
field
sanitation,
weed
control,
rotation,
play
vital
role
in
disease
management.
Insecticide
application,
use
yellow
sticky
traps,
whitefly
population
monitoring
are
important
components
integrated
pest
aimed
reducing
transmission.
Additionally,
development
deployment
resistant
cultivars
conventional
breeding
biotechnological
shown
promise
minimizing
infection
its
associated
losses.
Despite
these
efforts,
continues
pose
challenges
production,
necessitating
further
research
innovative
approaches.
Future
involve
integration
advanced
technologies
molecular
diagnostics,
genome
editing,
RNA
interference
enhance
resistance
varieties.
promoting
awareness
among
farmers
about
symptoms,
preventive
measures,
adoption
will
contribute
sustainable
production.
In
conclusion,
represents
affecting
productivity
sustainability.
emphasizes
need
concerted
efforts
develop
strategies.
By
combining
modern
techniques,
measures
can
be
implemented
secure
