Chitosan Nanoparticles as a Strategy for enhancing the Tropane Alkaloid Compounds Production and h6h Gene Expression in Hyoscyamus reticulatus Hairy Roots

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Background: Hyoscyamus reticulatus L. is a rich source of tropane alkaloids (TA) which widely used in medicine. In this study, hairy roots were obtained from two-week cotyledon explants H. by using the A7 strain Agrobacterium rhizogenes. The effects different concentrations chitosan nanoparticles (CNP) (0, 10, 30, 50 and 100 mg L⁻¹) as signaling molecule with two exposure times (24 48 h) on growth rate, antioxidant enzymes activity, phenol content, TA contents hyoscyamine-6-beta-hydroxylase (h6h) gene expression levels investigated. After extraction roots, High-performance liquid chromatography (HPLC) was to measure amount hyoscyamine scopolamine. Results: results showed that highest (1300.3 μg g⁻¹ FW) scopolamine (918.06 accumulated treated L⁻¹ CNP at 48h 10 24h, respectively. present effect on h6h also investigated semi-quantitative RT-PCR. observed at concentration 24h lowest control sample. Conclusions: Based these results, it concluded CNP can be an effective stimulant for production plant secondary metabolites, including TA, probably due stimulation TA.

Язык: Английский

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Navigating the challenges of engineering composite specialized metabolite pathways in plants

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels target metabolites, alternative strategies such as pathway engineering heterologous systems like microbes (e.g., bacteria and fungi) or cell‐free can be employed. Another approach is plant engineering, which aims to either enhance the native production original reconstruct model system. Although increasing metabolite promising strategy, plants exotic pose significant challenges for genetic manipulation. Effective requires comprehensive prior knowledge genes enzymes involved, well precursor, intermediate, branching, final metabolites. Thus, thorough elucidation biosynthetic closely linked successful metabolic host systems. In this review, we highlight recent advances engineering. We focus on efforts engineer complex, multi‐step pathways that require expression at least eight transient three stable transformation. Reports complex stably transformed remain relatively scarce. discuss major hurdles overcoming them, followed by an overview achievements, challenges, solutions reconstitution through Recent including computer‐based predictions offer platforms sustainable plants.

Язык: Английский

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The Occurrence, Uses, Biosynthetic Pathway, and Biotechnological Production of Plumbagin, a Potent Antitumor Naphthoquinone

Molecules, Год журнала: 2025, Номер 30(7), С. 1618 - 1618

Опубликована: Апрель 4, 2025

Plumbagin is an important naphthoquinone with potent anticancer properties besides multitudinous uses in healthcare. It produced a limited number of species and families but mostly the roots Plumbaginaceae family members. The biosynthetic pathway genes that regulate plumbagin synthesis are not completely known, details these being revealed. Several species, including Plumbago, Drosera, others, uprooted for extraction by pharmaceutical industries, leading to destruction natural habitats. industry therefore facing acute shortage plant material. This necessitates enhancing accumulation using suspensions hairy meet market demands. Many factors, such as aggregate size inoculum, stability culture, sequential effects elicitors, immobilization, permeabilization, have been demonstrated act synergistically markedly augment accumulation. Hairy root cultures can be used large-scale production, growth, accumulation, exploration their efficacy now imperative. secretion compounds into spent medium situ adsorption via resin has remarkable potential, this thoroughly exploited. Improvements quality biomass, selection cell lines, production bioreactors thus far sporadic, parameters need further In review, we report advances made relating importance stable line long-term cultures, plumbagin, its semicontinuous total recycling different types bioreactors. Such might pave way industrial exploitation. steps currently understood also aid us isolating relevant order examine overexpression or heterologous downregulation edit genome CRISPR-Cas9 technology enhance plumbagin. Its potential molecule mode action amply demonstrated, exploited clinics due insolubility water highly lipophilic nature. Plumbagin-loaded nanoemulsions, plumbagin–silver, albumin nanoparticle formulations overcome problems solubility tried improve bioavailability antiproliferative activities, discussed current paper.

Язык: Английский

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Enhancing secondary metabolite biosynthesis from ethnomedicinal plant Solanum nigrum L. through cytogenetically stable mass propagation, transgenic hairy root induction, and using different LED light and culture vessels for hairy root culture

Journal of Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Plant growth regulator studies and emerging biotechnological approaches in Artemisia annua L.: A comprehensive overview

South African Journal of Botany, Год журнала: 2025, Номер 181, С. 181 - 222

Опубликована: Апрель 21, 2025

Язык: Английский

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Efficient Production and Enhanced Accumulation of Valerenic Acid in Valeriana officinalis: Early Identification of High-Performing Hairy Root Clones and Pioneering Use of Hydrogen Peroxide as an Elicitor

Biocatalysis and Agricultural Biotechnology, Год журнала: 2024, Номер 60, С. 103319 - 103319

Опубликована: Июль 14, 2024

Язык: Английский

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Elicitation boosts withaferin A and withanolide A production in Withania somnifera (L.) Dunal hairy root cultures

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2024, Номер 158(2)

Опубликована: Авг. 1, 2024

Язык: Английский

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Hernandulcin Production in Elicited Hairy Roots of Phyla scaberrima: Toward Sustainable Production of a Non‐Caloric Sweetener with Nutraceutical Properties

Chemistry & Biodiversity, Год журнала: 2024, Номер 21(3)

Опубликована: Фев. 9, 2024

Abstract Herein we report on the generation of hairy root lines P. scaberrima able to produce hernandulcin (HE), a non‐caloric sweetener with nutraceutical properties. From ten different analyzed, three synthesized up 100 mg ⋅ L −1 HE under batch culture conditions standardized in this investigation. Adding elicitors (salicylic acid, chitin, Glucanex, polyethylene glycol) and biosynthetic precursors (farnesol (+)‐epi‐alpha‐bisabolol) significantly altered accumulation. Chitin Glucanex enhanced production from 130 160 , whereas farnesol (+)‐epi‐alpha‐bisabolol 165 200 without dependence biomass Improved cultures containing liquid Murashige & Skoog medium (MS; pH 7), added 4 % sucrose, 0.5 naphthaleneacetic 150 250 farnesol, at 25 °C (12 h light/12 darkness), triggered accumulation days. The efficiency each recombinant line is discussed.

Язык: Английский

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Improvement of sulforaphane production in hairy root cultures of broccoli (Brassica oleracea L. var. italica) by eliciting Myrosinase gene expression and its effect on breast cancer cells

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2024, Номер 158(1)

Опубликована: Июнь 26, 2024

Abstract Plant-derived hairy root cultures have the potential to be a significant biotechnological tool for producing valuable secondary metabolite compounds. This study deals with production of broccoli’s via mediation Agrobacterium rhizogenes and investigates effects different concentrations copper sulfate (CuSO 4 ) zinc (ZnSO as two elicitors on sulforaphane in broccoli's root. The expression Myrosinase ( MY gene, associated production, was quantified using real-time PCR. Moreover, cytotoxic effect treatments breast cancer cell line, MDA-MB-231, estimated transcription levels apoptosis-associated genes were also determined . highest amount produced at µM CuSO after 16 h ZnSO 8 elicitation. Overexpression gene found increased sulforaphane. All induced dose-dependent reduction viability cells. extracted from roots treated exerted higher MDA-MB-231 line than up-regulated Apoptosis Regulator Bax Proapoptotic Bcl2 Associated X, Caspase-3 , Caspase-8 Caspase-9, while down-regulating B-cell lymphoma 2 Bcl-2 (antiapoptotic) transcription. overall results showed an antiapoptotic extract derived MDAMB-231 cells, thereby establishing anticancer activity cultures.

Язык: Английский

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Development of Stephania tetrandra S. MOORE hairy root culture process for tetrandrine production

Journal of Biotechnology, Год журнала: 2024, Номер 394, С. 11 - 23

Опубликована: Авг. 14, 2024

Язык: Английский

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