Genome-wide identification and expression analysis of GRAS gene family in Eucalyptus grandis
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Июнь 18, 2024
Abstract
Background
The
GRAS
gene
family
is
a
class
of
plant-specific
transcription
factors
with
important
roles
in
many
biological
processes,
such
as
signal
transduction,
disease
resistance
and
stress
tolerance,
plant
growth
development.
So
far,
no
information
available
describes
the
functions
genes
Eucalyptus
grandis
.
Results
A
total
82
were
identified
amino
acid
lengths
ranging
from
267
to
817
aa,
most
EgrGRAS
had
one
exon.
Members
are
divided
into
9
subfamilies
different
protein
structures,
while
members
same
subfamily
have
similar
structures
conserved
motifs.
Moreover,
these
expanded
primarily
due
segmental
duplication.
In
addition,
cis
-acting
element
analysis
showed
that
this
was
involved
transduction
various
hormones,
development,
response.
qRT-PCR
data
indicated
18
significantly
responded
hormonal
abiotic
stresses.
Among
them,
expression
EgrGRAS13
,
EgrGRAS68
EgrGRAS55
up-regulated
during
treatment
period,
it
hypothesised
play
an
role
tolerance.
Conclusions
study,
phylogenetic
relationship,
domains,
-elements
patterns
analyzed,
which
filled
gap
identification
laid
foundation
for
analyzing
function
hormone
Язык: Английский
Genome-wide identification and expression analysis of GRAS transcription factors under cold stress in diploid and triploid Eucalyptus
Frontiers in Genetics,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 29, 2025
The
GRAS
[GRI
(Gibberellic
Acid
Insensitive),
RGA
(Repressor
of
GAI-3
mutant),
and
SCR
(Scarecrow)]
transcription
factors
play
a
pivotal
role
in
the
development
stress
responses
plants.
Eucalyptus
is
an
important
fast-growing
tree
species
worldwide,
yet
its
poor
cold
tolerance
limits
cultivation
range.
This
study
conducted
bioinformatics
analysis
grandis
family
investigated
expression
patterns
genes
different
ploidy
under
treatment.
identified
92
EgrGRAS
genes,
which
were
divided
into
eight
subfamilies.
Interspecies
synteny
found
that
E.
Populus
trichocarpa
have
more
syntenic
gene
pairs.
Chromosome
localization
revealed
90
to
be
unevenly
distributed
across
11
chromosomes.
Gene
structure
similar
intron-exon
structures
genes.
Protein
motif
proteins
within
same
subfamily
certain
structural
similarities.
physical
chemical
properties
encoded
by
vary,
but
ranges
amino
acid
numbers,
molecular
weights,
isoelectric
points
(pI)
are
those
from
other
species.
Subcellular
prediction
using
software
56
members
localized
nucleus,
with
few
cytoplasm,
chloroplasts,
mitochondria.
Tobacco
subcellular
experiments
verified
nuclear-localized
factor.
Cis
-acting
element
predicted
involved
growth
as
well
response
hormones,
light
induction,
low-temperature
stress.
Transcriptome
data
quantitative
real-time
PCR
(qRT-PCR)
diploid
triploid
urophylla
some
exhibited
upregulated
treatment
durations,
LISCL,
PAT1,
DELLA
subfamilies
significantly
Eucalyptus.
These
may
lays
foundation
for
breeding
cold-resistant
varieties.
Язык: Английский
Genome-Wide Identification, Expression, and Protein Interaction of GRAS Family Genes During Arbuscular Mycorrhizal Symbiosis in Poncirus trifoliata
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 2082 - 2082
Опубликована: Фев. 27, 2025
Arbuscular
mycorrhizal
(AM)
fungi
establish
mutualistic
symbiosis
with
most
land
plants,
facilitating
mineral
nutrient
uptake
in
exchange
for
photosynthates.
As
one
of
the
commercially
used
rootstocks
citrus,
Poncirus
trifoliata
heavily
depends
on
AM
absorption.
The
GRAS
gene
family
plays
essential
roles
plant
growth
and
development,
signaling
transduction,
responses
to
biotic
abiotic
stresses.
However,
identification
functional
characterization
genes
P.
remains
largely
unexplored.
In
this
study,
a
comprehensive
genome-wide
analysis
PtGRAS
was
conducted,
including
their
identification,
physicochemical
properties,
phylogenetic
relationships,
structures,
conserved
domains,
chromosome
localization,
collinear
relationships.
Additionally,
expression
profiles
protein
interaction
these
under
were
systematically
investigated.
result,
41
identified
genome,
classified
into
nine
distinct
clades.
Collinearity
revealed
seven
segmental
duplications
but
no
tandem
duplications,
suggesting
that
duplication
played
more
important
role
expansion
compared
duplication.
18
differentially
expressed
response
symbiosis,
orthologs
RAD1,
RAM1,
DELLA3
trifoliata.
Yeast
two-hybrid
(Y2H)
screening
further
PtGRAS6
PtGRAS20
interacted
both
PtGRAS12
PtGRAS18,
respectively.
interactions
subsequently
validated
through
bimolecular
fluorescence
complementation
(BiFC)
assays.
These
findings
underscored
crucial
trifoliata,
provided
valuable
candidate
improving
stress
resistance
citrus
molecular
breeding
approaches.
Язык: Английский
The GRAS gene family and its roles in pineapple (Ananas comosus L.) developmental regulation and cold tolerance
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 19, 2024
Pineapple
(Ananas
comosus
L.)
is
a
major
tropical
fruit
crop
with
considerable
economic
importance,
and
its
growth
development
are
significantly
impacted
by
low
temperatures.
The
plant-specific
GRAS
gene
family
plays
crucial
roles
in
diverse
processes,
including
flower
development,
as
well
stress
responses.
However,
the
role
of
pineapple
has
not
yet
been
systematically
analyzed.
In
this
study,
43
AcGRAS
genes
were
identified
genome;
these
distributed
unevenly
across
19
chromosomes
6
scaffolds
designated
AcGRAS01
to
AcGRAS43
based
on
their
chromosomal
locations.
Phylogenetic
analysis
classified
into
14
subfamilies:
OS19,
HAM-1,
HAM-2,
SCL4/7,
LISCL,
SHR,
PAT1,
DLT,
LAS,
SCR,
SCL3,
OS43,
OS4,
DELLA.
Gene
structure
revealed
that
60.5%
lacked
introns.
Expression
profiling
demonstrated
tissue-specific
expression,
most
predominantly
expressed
specific
floral
organs,
tissues,
or
during
particular
developmental
stages,
suggesting
functional
diversity
development.
Furthermore,
majority
induced
cold
stress,
but
different
members
seemed
play
distinct
short-term
long-term
adaptation
pineapple.
Notably,
PAT1
subfamily
preferentially
late
petal
upregulated
under
special
response.
contrast,
no
consistent
expression
patterns
observed
among
other
subfamilies,
various
regulatory
factors,
such
miRNAs,
transcription
cis-regulatory
elements,
may
contribute
functions
members,
even
within
same
subfamily.
This
study
provides
first
comprehensive
pineapple,
offers
valuable
insights
for
further
investigations
AcGRASs
clues
improving
resistance
breeding.
Язык: Английский
Optimization of an on-farm multiplication and sanitation technique for plantain banana
Fruits,
Год журнала:
2024,
Номер
79(2), С. 1 - 19
Опубликована: Июль 1, 2024
Язык: Английский
ZmGRAS46 negatively regulates flowering time in maize
GM crops & food,
Год журнала:
2024,
Номер
16(1), С. 83 - 96
Опубликована: Дек. 30, 2024
Flowering
time
is
an
important
factor
limiting
the
planting
area
of
maize
(Zea
may
L.).
Gibberellin
(GA)
can
regulate
plant
flowering
by
mediating
GA
signaling
pathway.
This
study
screened
significantly
down-regulated
gene
ZmGRAS46
early
mutant
transcriptomic
sequencing
(PRJNA788070)
in
previous
laboratory.
The
expression
pattern
analysis
shows
that
it
has
highest
level
stems.
stem
treatment
with
200
μmol/L
GA3
resulted
lowest
at
3
h.
Positive
plants
were
obtained
through
modified
Agrobacterium-mediated
genetic
transformation
maize.
results
showed
overexpression
delayed
maize,
and
editing
made
blossom
earlier.
In
addition,
could
increase
100-grain
weight.
provides
new
insights
into
molecular
mechanism
GRAS
regulating
flowering.
Язык: Английский
Molecular identification and functional characterization of a transcription factor GeRAV1 from Gelsemium elegans
BMC Genomics,
Год журнала:
2024,
Номер
25(1)
Опубликована: Янв. 2, 2024
Abstract
Background
Gelsemium
elegans
is
a
traditional
Chinese
medicinal
plant
and
temperature
one
of
the
key
factors
affecting
its
growth.
RAV
(related
to
ABI3/VP1)
transcription
factor
plays
multiple
roles
in
higher
plants,
including
regulation
growth,
development,
stress
response.
However,
G.
has
not
been
reported.
Results
In
this
study,
three
novel
GeRAV
genes
(
GeRAV1
-
GeRAV3
)
were
identified
from
transcriptome
under
low
stress.
Phylogenetic
analysis
showed
that
GeRAV1-GeRAV3
proteins
clustered
into
groups
II,
IV,
V,
respectively.
RNA-sequencing
(RNA-seq)
real-time
quantitative
PCR
(qRT-PCR)
analyses
indicated
expression
GeRAV2
was
increased
response
cold
Furthermore,
gene
successfully
cloned
leaf.
It
encoded
hydrophilic,
unstable,
non-secretory
protein
contained
both
AP2
B3
domains.
The
amino
acid
sequence
shared
high
similarity
81.97%
with
Camptotheca
acuminata
CaRAV.
Subcellular
localization
transcriptional
self-activation
experiments
demonstrated
nucleoprotein
without
self-activating
activity.
constitutively
expressed
leaves,
stems,
roots
,
highest
levels
roots.
addition,
rapidly
up-regulated
abscisic
(ABA),
salicylic
(SA),
methyl
jasmonate
(MeJA)
stresses,
suggesting
it
may
be
involved
hormonal
signaling
pathways.
Moreover,
conferred
improved
sodium
chloride
tolerance
Escherichia
coli
Rosetta
cells.
Conclusions
These
findings
provided
foundation
for
further
understanding
on
function
regulatory
mechanism
low-temperature
elegans.
Язык: Английский
Genome-Wide Identification and Characterization of GRAS Transcription Factor Family in Cultivated Hybrid Sugarcane ZZ1 (Saccharum officinarum) and Their Role in Development and Stress
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13470 - 13470
Опубликована: Дек. 16, 2024
GRAS
gene
family
plays
multifunctional
roles
in
plant
growth,
development,
and
resistance
to
various
biotic
abiotic
stresses,
belonging
the
plant-specific
transcription
factor
(TF)
family.
In
this
study,
a
genome-wide
survey
systematic
analysis
of
cultivated
hybrid
sugarcane
ZZ1
(Saccharum
officinarum)
with
economic
industrial
importance
was
carried
out.
We
identified
747
genes
complete
structural
domains
classified
these
into
11
subfamilies
by
phylogenetic
analyses,
exhibiting
diverse
range
molecular
weight
isoelectric
points,
thereby
indicating
broad
functional
spectrum.
Analysis
Protein
motif
structure
revealed
conserved
yet
variable
arrangement
motifs
within
TFs,
suggesting
its
potential
for
roles.
Furthermore,
identification
numerous
cis-regulatory
elements
TFs
promoter
sequence
analysis,
implying
their
complex
regulation
response
environmental
physiological
signals.
Tertiary
predictions
analyses
using
AlphaFold3
highlighted
flexibility
conservation
family,
disordered
regions
potentially
contributing
versatility.
Weighted
Gene
Co-expression
Network
(WGCNA)
provided
insights
ShGRAS21A
sugarcane’s
smut
infection.
This
comprehensive
investigation
not
only
uncovers
diversity
but
also
sheds
light
on
regulatory
stress
response.
The
findings
contribute
deeper
understanding
functions
lay
groundwork
future
studies
role
improvement
disease
resistance.
Язык: Английский