Perspectives of Genome Editing Mediated Haploid Inducer Systems in Legumes
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1154 - 1154
Опубликована: Янв. 29, 2025
Genome
editing-mediated
haploid
inducer
systems
(HISs)
present
a
promising
strategy
for
enhancing
breeding
efficiency
in
legume
crops,
which
are
vital
sustainable
agriculture
due
to
their
nutritional
benefits
and
ability
fix
nitrogen.
Traditional
is
often
slow
complicated
by
the
complexity
of
legumes’
genomes
challenges
associated
with
tissue
culture.
Recent
advancements
have
broadened
applicability
HISs
facilitating
reduction
duration
cycle.
By
integrating
genome
editing
technology
systems,
researchers
can
achieve
precise
genetic
modifications
rapidly
produce
homozygous
lines,
thereby
significantly
accelerating
development
desired
traits.
This
review
explores
current
status
future
prospects
legumes,
emphasizing
mechanisms
induction;
recent
breakthroughs;
existing
technical
challenges.
Furthermore,
we
highlight
necessity
additional
research
optimize
these
across
various
species,
has
potential
greatly
enhance
contribute
sustainability
production.
Язык: Английский
Genome-wide analysis and expression pattern profiling of the DUF789 gene family in soybean (Glycine max L.)
South African Journal of Botany,
Год журнала:
2025,
Номер
180, С. 1 - 11
Опубликована: Март 6, 2025
Язык: Английский
Comprehensive characterization and diversity analysis of the HIS1 gene family in rice subpopulations for herbicide resistance
BMC Plant Biology,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 24, 2025
Understanding
the
genetic
diversity
and
functional
roles
of
key
resistance
genes
is
crucial
for
developing
sustainable
weed
management
strategies
in
rice
cultivation.
HIS1
(HPPD
INHIBITOR
SENSITIVE
1)
confers
broad-spectrum
to
β-triketone
herbicides
rice.
However,
despite
its
importance,
family
HIS1-like
(HSLs)
remains
largely
uncharacterized.
Here,
we
identified
25
gene
members
across
four
subpopulations,
including
13
Nipponbare,
4
Minghui
63,
6
Zhenshan
97,
Nagina-22.
Phylogenetic
analysis
grouped
these
into
seven
distinct
subfamilies
(HIS1,
HSL1
~
HSL6).
While
HIS1,
HSL2,
HSL3
were
present
all
others
exhibited
subpopulation-specific
presence/absence,
underscoring
influence
evolutionary
pressures
on
family.
Haplotype
within
a
collection
475
accessions
revealed
natural
variation
only
three
genes:
HSL3,
with
japonica
exhibiting
high
conservation
genes,
while
indica
displayed
diversity,
forming
haplotypes
HSL2.
did
not
strongly
correlate
Benzobicyclon
(BBC)
resistance,
suggesting
that
variations
are
primary
determinants
herbicide
response.
Expression
profiling
under
BBC
treatment
ecotype-specific
regulation,
notable
upregulation
HSL5
BBC-resistant
accessions.
Our
tandem
duplication
as
major
driver
expansion
diversification
chromosome
The
response
points
potential
role
mediating
ecotypes.
Employing
an
reference
genome
could
further
enhance
insights
mechanisms.
This
comprehensive
enhances
understanding
patterns
functions
rice,
offering
valuable
knowledge
future
breeding
programs.
Not
applicable.
Язык: Английский
Genomic identification and expression profiling of DMP genes in oat (Avena sativa) elucidate their responsiveness to seed aging
BMC Genomics,
Год журнала:
2024,
Номер
25(1)
Опубликована: Сен. 16, 2024
The
Domain
of
unknown
function
679
membrane
protein
(DMP)
family,
which
is
unique
to
plants,
plays
a
crucial
role
in
reproductive
development,
stress
response
and
aging.
A
comprehensive
study
was
conducted
identify
the
DMP
gene
members
oat
(Avena
sativa)
investigate
their
structural
features
tissue-specific
expression
profiles.
Utilizing
whole
genome
transcriptome
data,
we
analyzed
physicochemical
properties,
structure,
cis-acting
elements,
phylogenetic
relationships,
conserved
(CS)
domains,
CS
motifs
patterns
AsDMP
family
A.
sativa.
Язык: Английский
Genome-Wide Identification and Expression Analysis of the DMP and MTL Genes in Sweetpotato (Ipomoea batatas L.)
Genes,
Год журнала:
2024,
Номер
15(3), С. 354 - 354
Опубликована: Март 12, 2024
Sweetpotato
(Ipomoea
batatas
L.)
is
a
strategic
crop
with
both
economic
and
energy
value.
However,
improving
sweetpotato
varieties
through
traditional
breeding
approaches
can
be
time-consuming
labor-intensive
process
due
to
the
complex
genetic
nature
of
as
hexaploid
species
(2n
=
6x
90).
Double
haploid
(DH)
breeding,
based
on
in
vivo
induction,
provides
new
approach
for
rapid
crops.
The
success
induction
achieved
by
manipulating
specific
genes.
Two
most
critical
genes,
DMP
(DUF679
membrane
proteins)
MTL
(MATRILINEAL),
have
been
shown
induce
production
several
species.
Here,
we
identified
characterized
genes
using
gene
family
analysis.
In
this
study,
5
IbDMPs
25
IbpPLAs.
IbDMP5
IbPLAIIs
(IbPLAIIκ,
IbPLAIIλ,
IbPLAIIμ)
were
potential
(HI)
sweetpotato.
These
results
provide
valuable
information
identification
function
HI
ideas
DH
lines.
Язык: Английский
Genome-wide identification of the oat DMP gene family and its expression analysis in response to seed aging
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 2, 2024
Abstract
Background:
The
Domain
of
unknown
function
679
membrane
proteins
(DMPs)
family,
as
a
green
plant-specific
protein,
plays
an
important
role
in
plant
reproductive
development,
stress
response
and
aging.
To
identify
the
DMP
gene
members
oat
(
AsDMP
)
to
investigate
their
family
structural
features
tissue
expression
profile
characteristics,
study
was
conducted.
Based
on
whole
genome
transcriptome
data,
this
investigation,
we
have
scrutinized
physicochemical
properties,
structure,
cisacting
elements,
phylogenetic
relationships,
conserved
(CS)
domains,
CS
motifs
patterns
oat.
Results
genes
were
found
distributed
across
17
chromosomal
scaffolds
with
33
members.
We
could
divide
into
five
subfamilies
based
relationships.
structure
suggests
that
oats
may
also
undergone
intron
loss
event
during
evolution.
Covariance
analysis
genome-wide
duplication/segmental
duplication
be
major
contributor
expansion
family.
Ka/Ks
selective
pressure
pairs
tend
over
evolutionary
time.
upstream
promoter
these
containing
several
cis-acting
elements
indicates
plausible
abiotic
hormone
induction.
Gene
pattern
according
data
revealed
participation
organ
development.
In
study,
AsDMP1
,
AsDMP19
AsDMP22
identified
potentially
regulating
seed
senescence,
can
used
candidate
for
longevity
anti-aging
germplasm
breeding
studies
provides
valuable
information
regulatory
mechanism
aging
process
germplasm,
theoretical
support
further
investigation
molecular
anti-aging.
Conclusions
is
involved
seeds,
which
first
report
potential
seeds.
Язык: Английский
Characterization, Evolution, Expression and Functional Divergence of the DMP Gene Family in Plants
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(19), С. 10435 - 10435
Опубликована: Сен. 27, 2024
The
DMP
(DOMAIN
OF
UNKNOWN
FUNCTION
679
membrane
protein)
domain,
containing
a
family
of
proteins
specific
to
green
plants,
is
involved
in
numerous
biological
functions
including
physiological
processes,
reproductive
development
and
senescence
Arabidopsis,
but
their
evolutionary
relationship
function
most
crops
remains
unknown.
In
this
study,
we
scrutinized
phylogenetic
relationships,
gene
structure,
conserved
domains
motifs,
promoter
regions,
loss/duplication
events
expression
patterns.
Overall,
240
DMPs
were
identified
analyzed
24
plant
species
selected
from
lower
plants
angiosperms.
Comprehensive
analysis
revealed
that
these
underwent
purifying
selection
could
be
divided
into
five
groups
(I–V).
structure
showed
it
may
have
undergone
an
intron
loss
event
during
evolution.
had
the
same
domains,
which
distinct
each
other
terms
number
DMPs;
group
III
was
largest,
closely
followed
by
V.
promotor
region
with
various
cis-regulatory
elements
predicted
potential
role
development,
hormone
induction
abiotic
stresses.
Based
on
transcriptomic
data,
profiling
primarily
expressed
organs
moderately
tissues.
Evolutionary
suggested
occurred
more
frequently
than
duplication
among
all
groups.
genome-wide
study
elucidates
species,
further
research
needed
many
validate
roles.
Язык: Английский
Multi-omics analysis reveals genetic architecture and local adaptation of coumarins metabolites in Populus
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 6, 2024
Accumulation
of
coumarins
plays
key
roles
in
response
to
immune
and
abiotic
stress
plants,
but
the
genetic
adaptation
basis
controlling
perennial
woody
plants
remain
unclear.
We
detected
792
SNPs
within
334
genes
that
were
significantly
associated
with
phenotypic
variations
15
single-metabolic
traits
multiple
comprehensive
index,
such
as
principal
components
(PCs)
metabolites.
Expression
quantitative
trait
locus
mapping
uncovered
337
eQTLs
expression
levels
132
genes.
Selective
sweep
revealed
55
candidate
have
potential
selective
signature
among
three
geographical
populations,
highlighting
biosynthesis
been
encountered
forceful
local
adaptation.
Furthermore,
we
constructed
a
network
seven
coordinately
regulate
biosynthesis,
revealing
regulatory
patterns
affecting
accumulation
Populus
tomentosa.
Validation
gene
drought-tolerated
population
DUF538
heterologous
transformation
experiments
verified
function
their
adapting
different
conditions
poplar.
Our
study
regulation
metabolic
Populus,
offered
clues
for
drought-tolerance
evaluation
regional
improvement
plants.
Язык: Английский
Genome-wide identification of the sugar beet (Beta vulgaris L.) DMP gene family and its potential role in abiotic stress
Genetic Resources and Crop Evolution,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 16, 2024
Язык: Английский
CRISPR/Cas9 and Anther Culture for Precision Double Haploid Line Production in Controlled Environments
Plant Breeding,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 9, 2024
ABSTRACT
The
development
of
mapping
populations
and
quantitative
trait
loci
(QTL)
analysis
face
constraints,
in
crops
exhibiting
male
sterility
self‐incompatibility
under
field
conditions.
Addressing
these
challenges
requires
the
integration
advanced
techniques,
including
temporal
alteration
or
excision
centromere
histone
H3
(CENH3)
protein
use
gene
editing
tools
such
as
MATRILINEAL
(MTL)
knockout.
Specifically,
this
can
be
achieved
through
Cas9/gRNA‐mediated
mutagenesis
Cas9/gRNA‐driven
promoter
expression
systems.
These
technologies
offer
efficient
means
to
advance
QTL
sterile
self‐incompatible
within
controlled
ecosystems.
doubled
haploid
(DH)
population,
traditionally
requiring
3
years
generation
time
via
anther
culture
method,
now
expedited
2–3
using
techniques
environmental
Notably,
DH
efficiently
generated
various
crops,
rice,
wheat,
maize,
barley
oats
by
leveraging
tools.
Among
tools,
novel
approach
CENH3
alteration/excision
emerges
highly
compared
MTL
knockout
mutation
Cas9/gRNA
expression.
However,
further
investigation
is
warranted
optimise
regeneration
double
enhance
Язык: Английский