In vitro and In silico investigation deciphering novel antifungal activity of endophyte Bacillus velezensis CBMB205 against Fusarium oxysporum

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 3, 2025

Endophytes from medicinal plants are potential biocontrol agents against Fusarium oxysporum f. sp. cubense (Foc), which is the causative fungus of banana wilt disease. In present study, endophytic bacterium was isolated Globba racemosa and their antagonistic activities Foc were studied, probable molecular mechanism antagonism predicted by docking studies. The 16SrRNA sequencing confirmed isolate to be Bacillus velezensis CBMB205 (EG2). isolates distortion fungal hyphae illustrated in SEM. metabolites identified FTIR, suggesting presence C-H, CH3 O-H groups. Two major such as β-amyrin dihydroxy octadecenoic acid (DA) LC-MS analysis. Molecular studies suggested that these showed binding with chitin synthase 1 1,3-glucan pathogenic fungi. energy (BE) interaction between synthase-1 (CS-1), (1,3-GS) estimated -10.17 kcal/mol − 9.5 kcal/mol, respectively. BE CS-1 1,3-GS determined -2.43 3.4 current study demonstrated EG2 towards provided a silico also provides insight into developing metabolite-based antifungal for various agricultural applications.

Язык: Английский

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Genomic Insight into a Potential Biological Control Agent for Fusarium-Related Diseases in Potatoes: Bacillus cabrialesii Subsp. cabrialesii Strain PE1

Horticulturae, Год журнала: 2024, Номер 10(4), С. 357 - 357

Опубликована: Апрель 4, 2024

Bacillus strain PE1, which was isolated from potatoes harvested in the Yaqui Valley, Mexico, evaluated as a potential biological control agent against Fusarium languescens. The draft genome sequence obtained through Illumina NovaSeq sequencing, revealing genomic size of 4,071,293 bp, with G + C content 44.13%, an N50 value 357,305 and 27 contigs. taxonomic affiliation confirmed by analyzing 16S rRNA gene overall relatedness indices (OGRIs) constructing phylogenomic tree based on whole genome, showed close relationship to cabrialesii subsp. cabrialesii. Genomic annotation using RAST Prokka identified 4261 coding DNA sequences (CDSs) distributed across 331 subsystems, highlighting genes associated biocontrol, stress response, iron acquisition. AntiSMASH 7.1 used for mining, seven biosynthetic clusters that potentially produce biocontrol-related metabolites. In vitro assays antagonistic activity PE1 languescens CE2, demonstrating its inhibit mycelial growth. study provides basis investigating B. potato production.

Язык: Английский

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Pseudomonas protegens ML15 and Trichoderma koningiopsis Tr21 Co-Culture: A potent strategy for Suppressing Fusarium cerealis infections in wheat through Augmented antifungal Metabolite production

Biological Control, Год журнала: 2024, Номер 198, С. 105621 - 105621

Опубликована: Сен. 13, 2024

Язык: Английский

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Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb

Applied Soil Ecology, Год журнала: 2025, Номер 206, С. 105915 - 105915

Опубликована: Фев. 1, 2025

Язык: Английский

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Comprehensive analysis of the mechanisms conferring resistance to phenamacril in the Fusarium species

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 11, 2025

The filamentous fungal genus Fusarium contains many species that cause catastrophic diseases in fruits, cereal, and vegetables. These substantial losses yield contaminate affected crops with toxins. This causes huge the agricultural sector threatens human animal health. most efficient approach to control spp. is fungicide application. Phenamacril a site-specific exerts its antifungal effect on sensitive It new developed targets graminearum by inhibiting myosin-5, an important protein growth disease development. Because of remarkable specificity, phenamacril regarded as environmentally benign. However, research findings have reported emergence resistance both field laboratory. article comprehensively analyzes mechanisms underlying phenamacril. We examine molecular, genetic, environmental factors contributing this resistance. emphasize importance continued integrating different approaches monitoring managing drug-resistant populations. Integrating current inventions inform strategies for sustainable practices, increase plant health, will contribute ongoing global efforts achieve food nutritional sustainability world's rapidly growing population while ensuring effectiveness fungicidal product.

Язык: Английский

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The occurrence of wheat crown rot correlates with the microbial community and function in rhizosphere soil

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 11, 2025

Wheat crown rot (WCR) is a significant soil-borne disease affecting wheat production worldwide. Understanding the impact of on structure and function microbial communities in rhizosphere soil can provide theoretical basis for mining biological control resources against WCR. In this study, soils with varying WCR severities (light, moderate, severe) were analyzed chemical properties, community composition functions using high-throughput sequencing. The results revealed that decreased pH, content available nitrogen phosphorus, abundance beneficial taxa such as Bacillus Streptomyces . Additionally, functional predictions showed adapted to by enhancing signaling pathways reducing their anabolic activity. From light occurrence, we isolated velezensis BF-237, whose was reduced Greenhouse experiments demonstrated BF-237 achieved efficiency 56.61% artificially inoculated sterilized 53.32% natural soil. This study clarifies structure, microorganisms, alongside identifying promising biocontrol agent. These findings contribute understanding pathogenesis offer practical its management.

Язык: Английский

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Farming practices affect soil's suppressiveness towards phytopathogens

Applied Soil Ecology, Год журнала: 2025, Номер 209, С. 106012 - 106012

Опубликована: Март 15, 2025

Язык: Английский

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Deciphering the rhizosphere microbiota composition of nature farming soybean (Glycine max L.) with different nodulation phenotypes

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 24, 2025

Nature farming, a sustainable agricultural method which avoids agrochemicals and untreated organic amendments, promotes both productivity ecological conservation. This system may foster unique plant-microbiota interactions for growth fitness; however, the microbiota of nature-farmed plants remains largely unexplored. Second, root nodule symbiosis (RNS) is crucial nitrogen fixation in legumes; its broader impact on rhizosphere assembly not well understood. study examined dynamics between impaired symbiosis, soil management, composition soybean (Glycine max L.). We evaluated characterized bacterial fungal communities by comparing wildtype soybeans (Enrei) with non-nodulating mutants (En1282) across four soils under conventional nature including fumigated unfumigated conditions. found that exhibited reduced compared wild-type plants, especially soils. Soil fumigation decreased microbial diversity reshaped community significant reduction plant nodulation all Restriction RNS increased soils, possibly as compensatory mechanism acquisition, whereas remained relatively stable. farming promoted beneficial microbes like Rhizobium, Trichoderma, Chloridium, favored Bacillus Aspergillus. Notably, differential enrichment analysis identified distinct associations each phenotype, Enrei predominantly enriched Pseudomonas, En1282 associated primarily oligotrophic microbes. Our sheds light complex interplay legume highlights significance eco-friendly methods cultivating healthy growth. The results paves way future strategies to manipulate microbiota, ultimately promoting robust systems reduce reliance chemical inputs.

Язык: Английский

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Soil Fusarium Antagonism and Resource Competition as Potential Mechanisms for Suppression of Bayoud Disease in Moroccan Date Palm Cultivation

Опубликована: Май 13, 2025

Bayoud disease, caused by Fusarium oxysporum f. sp. albedinis (Foa), poses a significant threat to date palm cultivation in North Africa. However, there is lack of in-depth studies on the soil microbial ecology governing disease development. This study explored outcomes nutrient competition among soil-borne Fusarium species and their antagonistic effects Foa. We assessed nutritional profiles isolates from three different soils: suppressive Marrakech (Fus_M) two conducive soils Errachidia (Fus_R) Zagora (Fus_Z), using Biolog SF-P2 plates. Co-inoculation tests were performed evaluate inhibitory activity each isolate Foa. Chemical analyses show that are rich nutrients, while poor nutrients. Fus_M exhibited greatest growth compared Fus_R, which showed similar Furthermore, Fus_Z significantly inhibited Foa 23% versus 17% 15% for Fus_R. Additionally, Fusarium (Fus_Z) shared 94% utilized was higher than Fus_R (88%) (84%). These results suggest suppression effect both interactions between pathogen. Overall, this highlights importance integrating an ecological approach develop effective management strategies against wilt palms.

Язык: Английский

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Identifying rhizosphere bacteria and potential mechanisms linked to compost suppressiveness towards Fusarium oxysporum

Environmental Microbiome, Год журнала: 2025, Номер 20(1)

Опубликована: Май 16, 2025

Soilborne fungal phytopathogens pose a significant threat to global food security. While chemical control remains an effective method for managing these pathogens, increasing regulations due health and environmental concerns, along with rising fungicide resistance, have restricted their use, underscoring the urgent need sustainable alternatives. The use of compost enhance soil fertility suppress plant diseases is well documented. Several studies underlined role microorganisms in disease suppression, but mechanisms facilitating this suppression remain unclear. We evaluated impact amendment on composition functional capacity rhizosphere microbiome cucumber plants (Cucumis sativus) inoculated Fusarium oxysporum f. sp. radicis-cucumerinum (FORC) under controlled greenhouse conditions using amplicon sequencing, shotgun metagenomic culture-based techniques. Compost significantly reduced FORC-induced relative non-amended treatments. FORC inoculation resulted shifts microbial (bacterial fungal) community plants, phenomenon was substantially less pronounced compost-amended plants. Specifically, sustained presence Actinomycetota (Streptomyces, Actinomadura, Saccharomonospora, Pseudonocardia, Glycomyces, Thermobifida) Bacillota (Planifilum, Novibacillus) that diminished without compost. These taxa contained myriad non-ribosomal peptides polyketides synthetases biosynthetic gene clusters (BGCs) putative antimicrobial iron-chelating functions. successfully isolated two Streptomyces strains from FORC-suppressing amended rhizospheres were almost identical bin2 (99% ortho ANI) metagenome assembled genome identified analysis. produced extracellular metabolites inhibited growth in-vitro BGCs encode compounds potential capacity. Based results presented study, we demonstrate alleviates dysbiosis microbiome, maintaining abundance specific bacterial taxa. groups may contribute through including iron chelation production antagonizing secondary metabolites.

Язык: Английский

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