Exploring the potential of seed inoculation with microbial consortia to mitigate drought stress in maize plants under greenhouse conditions

Plant and Soil, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Abstract Background and aims Plant-beneficial microbes may attenuate climate change-induced stresses on plants such as drought. We investigated the potential of beneficial microbial consortia (BMc) plant growth rhizosphere bacterial/archaeal community under Methods Seeds Zea mays B73 were inoculated with six plant-beneficial bacterial isolates either alone or combined in two three-member (BMc1, BMc2) before sowing loamy sandy substrates greenhouse. A known effective consortium (BMc3) was included positive control. Drought treatment established BMc treatments by omitting watering last five weeks period. The maize single determined. Colony-forming units (CFUs) inoculants evaluated selective plating, effects native assessed using 16S rRNA gene amplicon sequencing basal root tip grown loam. Results In both water conditions, CFUs inoculations higher at roots than tips. Under well-watered seed inoculation a isolate had no effect substrates. resulted shoot (but not root) compared to non-inoculated controls conditions zone most important driver for beta-diversity, followed while showed effect. Conclusion Our study suggests that has drought stress during growth.

Язык: Английский

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Testing plant growth promoting microorganisms in the field - a proposal for standards

Frontiers in Plant Science, Год журнала: 2024, Номер 14

Опубликована: Янв. 16, 2024

In the European Union and worldwide there are a burgeoning markets for plant growth promoting microorganisms (PGPM) other biological agents as soil improvers, bio-fertilizers, bio-stimulants, control or bio-pesticides. Microbial have major share in this development. The use of such products is often advertised with promise contributing to sustainable agricultural practices by increasing crop yield offering an alternative substitute decrease dependency agriculture on hazardeous agrochemicals. contrast registered microbial protection products, PGPM that marketed EU improvers biostimulants, not strictly required proven minimum efficacy levels under field conditions. Manufacturers only ensure these do pose unacceptable risks human, animal health, safety environment. Uniform guidelines comparable EPPO - standards (European Mediterranean Plant Protection Organisation) test trials available. This paper attempts fill gap. It proposes trial design implementation, well recommendations type scope data collection evaluation. Selected research papers from literature were evaluated analyze, whether what extent requirements already met. majority had clear experimental followed proper Frequent deficiencies low number tested environments species, insufficient site agronomic management description missing humidity temperature. Using suggested assumed increase expressive power products.

Язык: Английский

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Trichoderma consortium compost corncob promotes the growth of Pseudostellaria heterophylla and alleviates its diseases incidence via reshaping the soil microbiome and improving the soil nutrients under continuous monocropping conditions

Industrial Crops and Products, Год журнала: 2024, Номер 216, С. 118800 - 118800

Опубликована: Май 21, 2024

Язык: Английский

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Rhizosphere competent inoculants modulate the apple root–associated microbiome and plant phytoalexins

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Май 27, 2024

Abstract Modulating the soil microbiome by applying microbial inoculants has gained increasing attention as eco-friendly option to improve disease suppressiveness. Currently, studies unraveling interplay of inoculants, root-associated microbiome, and plant response are lacking for apple trees. Here, we provide insights into ability Bacillus velezensis FZB42 or Pseudomonas sp. RU47 colonize microhabitats modulate their microbiome. We applied two strains plants grown in soils from same site either affected replant (ARD) not (grass), screened establishment selective plating, measured phytoalexins roots 3, 16, 28 days post inoculation (dpi). Sequencing 16S rRNA gene ITS fragments amplified DNA extracted dpi different microhabitat samples revealed significant effects on fungal β-diversity root-affected rhizoplane. Interestingly, only ARD soil, most abundant bacterial amplicon sequence variants (ASVs) changed significantly relative abundance. Relative abundances ASVs affiliated with Enterobacteriaceae were higher rhizoplane reduced both inoculants. Bacterial communities root endosphere but presence was indicated. Interestingly previously unobserved, responded increased phytoalexin content roots, more pronounced grass than soil. Altogether, our results indicate that rhizosphere competent, modulated perceived plants, which could make them interesting candidates an mitigation strategy ARD. Key points • Rhizosphere competent (mainly fungi) Inoculants abundance stronger

Язык: Английский

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Shift in the soil rhizobacterial community for enhanced solubilization and bioavailability of phosphorus in the rhizosphere of Allium hookeri Thwaites, through bioaugmentation of phosphate-solubilizing bacteria

3 Biotech, Год журнала: 2024, Номер 14(8)

Опубликована: Июль 27, 2024

Язык: Английский

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Roots of resilience: Optimizing microbe‐rootstock interactions to enhance vineyard productivity

Plants People Planet, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Societal Impact Statement Grape production relies signifcantly on agrochemicals, such as fertilizers and pesticides, to sustain vine health yield. However, excessive or improper use of these inputs leads detrimental environmental effects, including soil degradation, water contamination, biodiversity decline. To address this, research must explore sustainable alternatives. Enhancing the symbiotic interactions between grapevine rootstocks beneficial microorganisms offers a viable pathway. By fostering natural relationships, producers scientists can develop environmentally viticulture practices that strengthen resilience without compromising productivity. This approach also supports broader transition regenerative, ecologically balanced agricultural systems. Summary The productivity vineyards are influenced by complex surrounding microbiome. Emerging has highlighted pivotal role microbe‐rootstock alliances in modulating nutrient acquisition, water‐use efficiency, pathogen resistance. leveraging potential microorganisms, viticulturists optimize vineyard management enhance overall productivity, stability, sustainability. Through strategic selection with enhanced mycorrhizal associations targeted introduction plant growth‐promoting rhizobacteria, growers support adapting challenging conditions. Aditionally, manipulating rhizosphere microbiome, through techniques biofertilization reverse microdialysis, foster development robust, disease‐suppressive communities safeguard vines against biotic abiotic stressors. Integrating microbiome‐centric approaches into comprehensive strategies, potentially future rootstock genetic improvement programs, holds promise for improving grape yield, quality, long‐term viticultural

Язык: Английский

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Combined use of humic acids and Trichoderma harzianum as sustainable alternatives to alliviate salt stress in bell pepper

Brazilian Journal of Biology, Год журнала: 2025, Номер 85

Опубликована: Янв. 1, 2025

Abstract Bell pepper is a vegetable with beneficial properties for human nutrition. However, salinity an abiotic factor affecting bell yield in arid and semi-arid areas of Peru. The objective was to determine the combined effect humic acid (HA) Trichoderma harzianum (TH) as sustainable alternatives increase peppers subjected salt stress. experiment carried field out during 2023 2024 seasons design 2 x 3 factorial scheme, referring electrical conductivity (EC) irrigation water (0 4 dSm-1), HA (0, 15 30 L ha-1) TH 1 kg ha-1). Agronomic yield-related parameters were analyzed. results showed that saline soil accompanied by irrigations 0 dS m-1 EC, significant decrease agronomic concentration sodium chlorine leaves. application ha-1 plant height (86.53 y 86,42%), higher root dry weight (95.44 95.32%) (90.46 90.41%), also, greatly improved fruit length 64.6 63.74%) width (58.47 57.31%), significantly increased content per (91.49 91.30%), (93.29 93.15%) total (89.54 89.23%) relation plants It also increases potassium calcium leaves (37.88 48.71%), K+/ Na+ ratio (72.07 74.93%) proline (58.60 59.31%) superior control, defense mechanism against Therefore, dose acids increased, shows tolerance salinity, being alternative reduce stress

Язык: Английский

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Inoculation of wild type or EPS mutant Bacillus subtilis does not alter rhizocompetence, soil EPS, and tomato growth in the absence of stress

Rhizosphere, Год журнала: 2025, Номер unknown, С. 101076 - 101076

Опубликована: Апрель 1, 2025

Язык: Английский

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Selective recruitment of beneficial microbes in the rhizosphere of maize affected by microbial inoculants, farming practice, and seasonal variations

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Background Plant beneficial microorganisms as inoculants can improve crop performance, but factors affecting their impact on plant performance under field conditions remain unclear, thereby limiting use in farming. Here, we investigated how farming practices (e.g. tillage and N-fertilization intensity) growing seasons influenced the of a microorganism consortium (BMc: Trichoderma, Bacillus, Pseudomonas strains) maize affected rhizosphere competence each BMc strain. In addition, tested whether affects resident microbiome performance. two (2020 2021), assessed inoculation growth, nutritional status, gene expression, different at flowering stage. Results Inoculated strains successfully colonized independent practice. improved growth iron uptake 2020, regardless These effects co-occurred with lower precipitation levels 2020 compared to 2021. reduced expression several stress-related genes for drought. An increased by BMc-inoculated plants was observed associated upregulation ZmNAS3, which is linked uptake. Therefore, mitigated drought maize. The microbial communities were altered both years, patterns responder taxa differed between seasons. Metagenome analysis revealed that more encoding biosurfactants siderophores) enriched than Moreover, identified bacterial fungal positively relative abundance these significantly due while they showed overall higher abundances 2021, inoculation. We mapped sequences publicly available genomes verified occurrence various traits genomes. Conclusions Overall, show season determined effect shaping composition function findings highlight importance complex interplay abiotic stress conditions.

Язык: Английский

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Plant-beneficial bacteria are promoted in pasture-crop rotations in the Uruguayan Pampa, contributing to soil health and crop performance

Frontiers in Bacteriology, Год журнала: 2025, Номер 4

Опубликована: Май 21, 2025

Introduction Understanding how pasture-crop rotation design influences long-term soil health and microbial dynamics is crucial for sustainable agroecosystems. Pasture-crop rotations may alleviate degradation, but their effects or legacy on rhizosphere microbiomes, including potential recruitment of plant-beneficial microorganisms, link to crop yield need be better understood. This study examines land use intensity grazed pasture influence prokaryotic diversity, composition, functionality, productivity. Methods A gradient intensities ranging from continuous cropping (CCG), short (SR) long (LR) rotations, permanent improved (PIP), natural grassland (NGL) were sampled in a field experiment established 1995 the Uruguayan Pampa. Moreover, two stages rotation, one year after sown with sorghum (SRS, LRS) years soybean (SRG, LRG), studied assess persistence pasture-derived legacies. Soil physicochemical biological properties measured evaluate along Bulk soil, soybean, communities analyzed across using 16S rRNA gene amplicon sequencing functional assays bacterial isolates. Results showed partial retention assessed through selected properties, i.e. organic C, total N, extractable protein content higher LRG compared CCG, while an intermediate response was observed SRG. LR preserved legacy, maintaining bulk community composition similar PIP distinct SR converged CCG diverged PIP. Soybean diversity strongly shaped by type inoculation Bradyrhizobium elkanii , overriding intensification legacy. Key taxa ( Streptomyces, Solibacillus, Sphingomonas ) linked functionality. Linking data rhizobacterial isolates that Pseudomonas Bacillus Microbacterium all exhibiting multiple activities vitro enriched rotations. Discussion highlights design, particularly duration plant services health, contributing towards development resilient

Язык: Английский

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