Enhancing cotton whitefly (Bemisia tabaci) detection and counting with a cost-effective deep learning approach on the Raspberry Pi DOI Creative Commons

Feng Zhen,

Nan Wang, Ying Jin

и другие.

Plant Methods, Год журнала: 2024, Номер 20(1)

Опубликована: Окт. 20, 2024

Abstract Background The cotton whitefly ( Bemisia tabaci ) is a major global pest, causing significant crop damage through viral infestation and feeding. Traditional B. recognition relies on human eyes, which requires large amount of work high labor costs. pests overlapping generations, reproductive capacity, small size, migratory behavior present challenges for the real-time monitoring early warning systems. This study aims to develop an efficient, high-throughput automated system detection whiteflies. In this work, novel tool fast identification quantification was developed based deep learning-based model. approach enhances effectiveness control by facilitating earlier its establishment in cotton, thereby allowing quicker implementation management strategies. Results We compiled dataset 1200 annotated images whiteflies leaves, augmented using techniques like flipping rotation. modified YOLO v8s model replacing C2f module with Swin-Transformer introducing P2 structure Head, achieving precision 0.87, mAP 50 0.92, F1 score 0.88 ablation studies. Additionally, we employed SAHI image preprocessing integrated algorithm Raspberry Pi, GUI-based visual interface. Our preliminary analysis revealed higher density leaves afternoon middle-top, middle, middle-down plant sections. Conclusion Utilizing enhanced learning model, have achieved precise counting whiteflies, enabling application hardware devices Pi. highly suitable research requiring accurate including phenotypic analyses. Future will focus deploying such equipment fields manage infestations.

Язык: Английский

Plant resistance to the whitefly Bemisia tabaci is compromised in salt-stressed Capsicum DOI Creative Commons
Lotte Caarls,

Faith Enigimi,

Wendy van ‘t Westende

и другие.

Environmental and Experimental Botany, Год журнала: 2025, Номер unknown, С. 106101 - 106101

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Integrating AI detection and language models for real-time pest management in Tomato cultivation DOI Creative Commons
Yavuz Selim Şahin, Nimet Sema Gençer, Hasan Şahin

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Фев. 21, 2025

Tomato (Solanum lycopersicum L.) cultivation is crucial globally due to its nutritional and economic value. However, the crop faces significant threats from various pests, including Tuta absoluta, Helicoverpa armigera, Leptinotarsa decemlineata, among others. These pests not only reduce yield but also increase production costs heavy reliance on pesticides. Traditional pest detection methods are labor-intensive prone errors, necessitating exploration of advanced techniques. This study aims enhance in tomato using AI-based language models. Specifically, it integrates YOLOv8 for segmentation tasks ChatGPT-4 generating detailed, actionable insights detected pests. was chosen superior performance agricultural detection, capable processing large volumes data real-time with high accuracy. The methodology involved training model images plant damage. achieved a precision 98.91%, recall 98.98%, mAP50 98.75%, mAP50-95 97.72% tasks. For tasks, 97.47%, 98.81%, 99.38%, 95.99%. metrics demonstrate improvements over traditional methods, indicating model's effectiveness. integration further enhances system by providing detailed explanations recommendations based approach facilitates expert consultation, making management accessible untrained producers, especially remote areas. study's results underscore potential combining models revolutionize practices. Future research should focus these domain-specific improve accuracy reliability. Additionally, addressing computational limitations personal devices will be broader adoption. promises democratize information access, promoting more resilient, informed, environmentally conscious farming.

Язык: Английский

Процитировано

1
The biochemical and molecular mechanisms of plants: a review on insect herbivory DOI Creative Commons
Afeez Adesina Adedayo, Richard O. Musser,

Mari Aanaenson

и другие.

Plant Signaling & Behavior, Год журнала: 2024, Номер 20(1)

Опубликована: Дек. 26, 2024

Biochemical and molecular mechanisms have been essential to reduce various insect attacks on plants. The biochemical methods are wide involving direct indirect defenses. defensive chemical substances secreted effectively the wound caused by herbivores (insects phytopathogens) Plants responded producing VOCs which draw natural enemies of insects phytopathogens. progress observed in cognition stimulus plants their potential control responses is characterized modification shifts our attention development endogenous resistance preserving crops. main objective implementing a biotechnological mechanism crop production employ durable multimechanistic alternatives pests via plant produces upon encountering attack.

Язык: Английский

Процитировано

4
Temperature Requirements Can Affect the Microbial Composition Causing Sour Rot in Grapes DOI Creative Commons
Chiara Brischetto, Vittorio Rossi, Irene Salotti

и другие.

Environmental Microbiology Reports, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 27, 2025

ABSTRACT Sour rot (SR) is a late‐season non‐Botrytis affecting grapevines, resulting from complex interplay of microorganisms, including non‐ Saccharomyces yeasts and acetic acid bacteria. Nonmicrobial factors contributing to disease development encompass vectors (e.g., Drosophila spp.), the presence wounds or microcracks on grape berry surfaces, environmental conditions during ripening. The microbial complexes within SR‐affected grapes exhibit variability among different bioclimates seasons, with certain microorganisms predominating under specific conditions. This study examined influence microbiome composition associated bunches, utilising data 41 locations across three distinct Italian bioclimates. We selected nine yeast two bacterial species frequently isolated sour‐rotted for analysis. growth responses these temperature were assessed by categorising them into four ecophysiological clusters. Furthermore, we analysed distribution their respective clusters results indicate that microbiomes involved in SR can vary according bioclimatic grape‐growing area. Further research required comprehend ecological requirements define niches understand geographical epidemiology, enhance management strategies.

Язык: Английский

Процитировано

0
Screening of Capsicum spp. accessions for resistance to whitefly Bemisia tabaci MEAM1 affecting adult survival and nymphal development DOI Creative Commons

Erin Puspita Rini,

Dania Oktihafifah,

Miriam Strijker

и другие.

Euphytica, Год журнала: 2025, Номер 221(6)

Опубликована: Май 16, 2025

Abstract Whitefly ( Bemisia tabaci (Genn.) is an important pest in pepper Capsicum spp.) production, not only because infestations lower the quality of fruits but also whiteflies transfer viruses. Therefore, resistant varieties are urgently needed. In this research, we searched for accessions to B. MEAM1. Forty underwent screening through a no-choice clip cage assay, evaluating adult survival and oviposition rate female whiteflies. Accessions with low were validated by assessing whitefly population buildup whole plant assay. Lastly, nymphal development scored three most accessions. Accession Zuchtmaterial Nr.1 demonstrated ability reduce reproduction halted at egg first stage, thus repressing buildup. Additionally, observed that glandular trichomes type VI found exhibited negative correlation survival, rate, number all stages Thus, conclude Nr. 1 could serve as useful source resistance, possible role resistance mechanism.

Язык: Английский

Процитировано

0
Influence of Physical and Morphological Factors On the Preference and Colonization of Bemisia Tabaci MED in Soybean Genotypes DOI
Ana Paula Santana Lima, Edson Luiz Lopes Baldin, Thaís Lohaine Braga dos Santos

и другие.

Deleted Journal, Год журнала: 2024, Номер 76(2), С. 447 - 460

Опубликована: Фев. 19, 2024

The whitefly, Bemisia tabaci Mediterranean (MED), is an invasive pest of several crops, including soybeans. objective this study was to evaluate the resistance soybean genotypes B. MED, in addition influence possibly related physical and morphological factors. A no-choice test carried out with 90 genotypes. Subsequently, 35 materials were selected for further multiple-choice tests. Trichomes leaf color plants observed, aim correlating these factors preference colonization MED. KS 4202, TMG 1188 RR, M 7739 IPRO, 65l65 PI 229358 least preferred by adults In test, lowest numbers eggs nymphs per square centimeter observed Dowling, 229358, IAC 24, 4202. 19, 1288 1182 99R09, 2375 IPRO presented assay. Plants higher trichome density MED and, consequently, more heavily colonized insects. leaves lower luminosity reduced green yellow intensity attractive whiteflies. summary, RR exhibited both assays, thus indicating their potential as promising sources

Язык: Английский

Процитировано

2
Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance DOI Creative Commons
Diana Naalden, Wannes Dermauw, Aris Ilias

и другие.

Molecular Plant-Microbe Interactions, Год журнала: 2023, Номер 37(2), С. 98 - 111

Опубликована: Дек. 5, 2023

The phloem-feeding insect Bemisia tabaci is an important pest, responsible for the transmission of several crop-threatening virus species. While feeding, secretes a cocktail effectors to modulate plant defense responses. Here, we present set proteins identified in artificial diet on which B. was salivating. We subsequently studied whether these candidate can play role immune suppression. Effector G4 most robust suppressor induced- reactive oxygen species (ROS) response Nicotiana benthamiana. In addition, able suppress ROS production Solanum lycopersicum (tomato) and Capsicum annuum (pepper). localized predominantly endoplasmic reticulum N. benthamiana leaves colocalized with two target tomato: REF-like stress related protein 1 (RSP1) meloidogyne-induced giant cell DB141 (MIPDB141). Silencing MIPDB141 tomato reduced whitefly fecundity up 40%, demonstrating that involved susceptibility tabaci. Together, our data demonstrate effector impairs immunity whiteflies by interfering via interaction MIPDB141. [Formula: see text] Copyright © 2024 Author(s). This open access article distributed under CC BY-NC-ND 4.0 International license .

Язык: Английский

Процитировано

1
Assessment of tomato genotypes against the whitefly, Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) and Tomato leaf curl virus under controlled condition DOI

MK Ponselvakumari,

M. Murugan,

C. Chinniah

и другие.

International Journal of Tropical Insect Science, Год журнала: 2024, Номер 44(2), С. 581 - 593

Опубликована: Фев. 5, 2024

Язык: Английский

Процитировано

0
Transgenic Tomato Strategies Targeting Whitefly Eggs from Apoplastic or Ovary-Directed Proteins DOI Creative Commons

Natalie S. Thompson,

Aliya Fathima Anwar,

David Krum

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

Abstract Background Transgenic plants expressing proteins that target the eggs of ubiquitous plant pest Bemisia tabaci (whitefly) could be an effective insecticide strategy. Two approaches for protein delivery are assessed using mCherry reporter gene in transgenic tomato plants, while accommodating autofluorescence both plant, phloem-feeding whitefly and pedicle-attached eggs. Results Both strategies were segregated to homozygous genotype digital PCR. The first strategy uses a glycotransferase secretion signal peptide. Despite bright apoplastic accumulation, is not evident second targets in vivo eggs, where transgene was fused transduction domain (PTD) facilitate uptake into hemolymph as well synthetic vitellogenin ovary-targeting sequence. Phloem-specific expression fusion achieved from Commelina viral promoter. Accumulation sufficient observed females feeding on these nor their subsequently laid non-transgenic plants. Egg protection may mediated by protease activity which macerated Conclusions proved desired tissue-specific tomato, but insufficiently sensitive allow localization whiteflies or Segregated lines important drawing conclusions. implications observations possible pest-control including preliminary analogous chitinase constructs discussed.

Язык: Английский

Процитировано

0
Transgenic tomato strategies targeting whitefly eggs from apoplastic or ovary-directed proteins DOI Creative Commons

Natalie S. Thompson,

Aliya Fathima Anwar,

David Krum

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 27, 2024

Abstract Background Transgenic plants expressing proteins that target the eggs of ubiquitous plant pest Bemisia tabaci (whitefly) could be an effective insecticide strategy. Two approaches for protein delivery are assessed using mCherry reporter gene in transgenic tomato plants, while accommodating autofluorescence both plant, phloem-feeding whitefly and pedicle-attached eggs. Results Both strategies were segregated to homozygous genotype digital PCR. The first strategy uses a glycotransferase secretion signal peptide. Despite bright apoplastic accumulation, is not evident second targets vivo eggs, where transgene was fused transduction domain (PTD) facilitate uptake into hemolymph as well synthetic vitellogenin ovary-targeting sequence. Phloem-specific expression fusion achieved from Commelina viral promoter. Accumulation sufficient observed females feeding on these nor their subsequently laid non-transgenic plants. Egg protection may mediated by protease activity which macerated Conclusions proved desired tissue-specific tomato, but insufficiently sensitive allow localization whiteflies or Segregated lines important drawing conclusions. implications observations possible pest-control including preliminary analogous chitinase constructs discussed.

Язык: Английский

Процитировано

0