Abstract
There
has
been
a
significant
increase
in
cover
crop
adoption,
with
land
use
nearly
doubling
over
the
last
decade.
Winter
legume
crops
provide
several
ecosystem
services,
such
as
weed
suppression
and
reducing
soil
erosion,
while
serving
an
excellent
nitrogen
source
for
subsequent
cash
crops.
Hairy
vetch
(
Vicia
villosa
R.),
crimson
clover
Trifolium
incarnatum
),
winter
pea
Pisum
sativum
L.)
are
three
major
annual
United
States.
However,
varying
survival
rates
have
reduced
their
reliability
compared
hardy
grasses
like
cereal
rye
Secale
cereale
).
cultivars
selected
bred
pea,
which
also
used
food
forage
crops,
but
fewer
breeding
efforts
toward
cold
tolerance
made
hairy
or
clover.
Despite
current
efforts,
all
species
can
suffer
from
damage
hardiness
zone
6
below.
Developing
requires
multifaceted
approach,
including
cultivar
selection
hybridization,
quantitative
trait
locus
isolation,
management
practice
improvement,
identification
of
new
sources
hardiness.
Cold
acclimation,
deacclimation
resistance,
reacclimation
potential
possible
mechanisms
to
explore
these
be
evaluated
field
controlled
environments
using
visual
scoring,
chlorophyll
fluorescence,
ion
leakage
assays.
Life,
Год журнала:
2025,
Номер
15(2), С. 319 - 319
Опубликована: Фев. 19, 2025
The
genus
Camphora
encompasses
species
of
significant
ecological
and
economic
importance,
such
as
C.
parthenoxylon
officinarum,
which
exhibit
distinct
phenotypic
traits
stress
responses.
This
study
seeks
to
elucidate
the
molecular
basis
cold
tolerance
through
comparative
transcriptomic
analysis
complemented
by
physiological
characterization.
RNA
sequencing
revealed
6123
differentially
expressed
genes
between
two
species,
with
enriched
pathways
related
stress,
oxidative
carotenoid
biosynthesis,
photosynthesis.
Key
genes,
annexin
D5,
chlorophyll
a/b-binding
protein,
early
light-induced
protein
1,
9-cis-epoxycarotenoid
dioxygenase,
were
identified
critical
regulators
frost
resistance,
photosynthetic
efficiency,
biosynthesis.
Functional
enrichment
analyses
highlighted
involvement
signal
transduction,
membrane
stabilization,
secondary
metabolism
in
adaptive
Physiological
assays
supported
these
findings,
showing
higher
content
enhanced
antioxidative
enzyme
activities
parthenoxylon.
These
results
provide
valuable
insights
into
genetic
biochemical
mechanisms
underlying
adaptation
offer
promising
targets
for
enhancing
resilience
economically
plants.
Plant Cell & Environment,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 28, 2025
ABSTRACT
Cold
stress
is
the
major
abiotic
factor
limiting
crop
productivity.
However,
compared
to
model
plant
Arabidopsis
Thaliana
,
crucial
genes
and
underlying
molecular
mechanisms
involved
in
soybean
cold
remain
underexplored.
Here,
we
investigate
two
national
cultivars,
HH43
HX3,
bred
from
northeast
southwest
regions
of
China,
which
exhibit
significant
differences
tolerance.
Morphological
biochemical
examinations
show
that,
HH43,
HX3
exhibits
delayed
wilting,
reduced
oxidative
damage,
elevated
antioxidant
enzyme
activities
under
low
temperature
(4°C).
Transcriptomic
analyses
distinct
patterns
between
displays
a
rapid
gene
response,
while
gradual
increase.
Particularly,
identify
MEblue
MEgreen
modules
related
construct
their
GENIE3
networks.
Key
TFs
such
as
ATAF1
its
potential
targets
were
identified,
likely
contribute
tolerance
HX3.
Moreover,
proteomic
reveal
broader
more
sustained
protein
upregulation
associated
with
maintaining
metabolic
activity
cellular
homeostasis
cold‐tolerant
limited
response
was
observed
cold‐sensitive
degradation
certain
early
responsive
proteins.
These
findings
provide
substantial
resources
for
further
functional
research
breeding
cultivars.
Ecology and Evolution,
Год журнала:
2025,
Номер
15(4)
Опубликована: Март 30, 2025
ABSTRACT
Climate
change‐induced
heat
waves
often
reduce
seed
yields
and
quality
via
high‐temperature
effects
in
the
gametophytic
phase.
Yet,
contrast
to
model
crop
species,
ability
of
pollen
ovules
adapt
or
acclimate
stress
wild
plants
remains
poorly
understood.
To
address
this
gap,
we
examined
adaptation
acclimation
potential
six
traits
11
Silene
vulgaris
populations
across
a
temperature
gradient
Europe.
First,
cultivated
common
garden
reveal
differences
indicative
adaptation.
Next,
assessed
their
by
subjecting
flowering
two
chronic
(CHS)
treatments:
moderate
(35°C/30°C)
severe
(40°C/35°C)
for
18
days.
Also,
estimated
CHS
on
quantity
quality.
The
experiment
showed
no
intraspecific
variation
gradient,
suggesting
these
may
not
influence
reproductive
local
habitats.
During
CHS,
female
gametophyte
was
less
temperature‐sensitive
than
male.
Moderate
led
larger
ovaries
with
more
large‐sized
ovules,
while
reduced
ovule
numbers
but
increased
size.
Both
treatments
decreased
grain
numbers,
size,
anther
length,
causing
greater
reductions.
These
reductions
lower
yield
Under
both
treatments,
did
vary
along
except
size
under
which
warmer
climates.
Our
findings
revealed
lack
mechanisms
(except
size)
gradient.
suggest
that
rely
alternative
strategies,
such
as
shifts
physiology
biochemistry
phenology,
respond
thermal
associated
waves.
