Integrative phenotyping approaches to unmask the Phyb-PIF4 pathway in Arabidopsis thaliana reproductive organs at high ambient temperatures

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 15, 2024

Abstract Background The increasing ambient temperature significantly impacts plant growth, development, and reproduction. Uncovering the temperature-regulating mechanisms in plants is of high importance, not only for boosting our biology knowledge but also assisting breeders improving resilience to these stress conditions. Numerous studies on molecular by which regulate responses revealed that employ distinct transcription factors thermomorphogenesis specific each tissue type. A significant discovery this field was identification PHYTOCHROME-INTERACTING FACTORs (PIFs) as key regulators during vegetative growth. PIF4, a regulator auxin-mediated signaling pathways, crucial controlling high-temperature responses. Results In study, we screened wild type several PhyB-PIF4 pathway Arabidopsis mutant lines combined integrative phenotyping platforms root soil, shoot, inflorescence, seed. We demonstrated differentially reproductive organs through pathway. Suppression components mimics response wild-type plants. identified correlative between shoot tissues. This automated complemented monitoring changes transcript levels organs. Transcriptomic profiling pistils from grown under elements may provide clues behind temperature-induced reduced fertilization rate, such downregulation auxin metabolism, upregulation genes involved signalling, miRNA156 miRN160 pollen tube attractants. Conclusions Thermomorphogenesis uniquely controlled different tissues at developmental stages. have help determine temperatures reproduction processes.

Язык: Английский

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The O-Fucosyltransferase SPINDLY Attenuates Auxin-Induced Fruit Growth by Inhibiting ARF6 and ARF8 binding to Coactivator Mediator Complex in Arabidopsis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 27, 2024

ABSTRACT The phytohormone auxin plays a pivotal role in promoting fruit initiation and growth upon fertilization flowering plants. Upregulation of signaling by genetic mutations or exogenous treatment can induce seedless formation from unpollinated ovaries, termed parthenocarpy. Recent studies suggested that the class A AUXIN RESPONSE FACTOR6 (ARF6) ARF8 Arabidopsis play dual functions first inhibiting when complexed with unidentified corepressor IAA protein(s) before pollination, later after as ARF dimers. However, whether how posttranslational modification(s) regulate ARF6- ARF8-mediated were unknown. In this study, we reveal both ARF6 are O -fucosylated their middle region (MR) SPINDLY (SPY), novel nucleocytoplasmic protein O- fucosyltransferase, which catalyzes addition fucose moiety to specific Ser/Thr residues target proteins. Epistasis, biochemical transcriptome analyses indicated downstream SPY, but more predominant parthenocarpic growth. Intriguingly, two ARF6/8-interacting proteins, co-repressor IAA9 MED8, subunit coactivator Mediator complex, also SPY. Biochemical assays demonstrated SPY-mediated -fucosylation these proteins reduced ARF-MED8 interaction, led enhanced transcription repression activity ARF6/8-IAA9 complex impaired transactivation activities ARF6/8. Our study unveils SPY attenuating auxin-triggered through modulation key factors, MED complex.

Язык: Английский

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Integrative phenotyping analyses reveal the relevance of the phyB-PIF4 pathway in Arabidopsis thaliana reproductive organs at high ambient temperature

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Июль 29, 2024

Abstract Background The increasing ambient temperature significantly impacts plant growth, development, and reproduction. Uncovering the temperature-regulating mechanisms in plants is of high importance, for our fundamental understanding thermomorphogenesis, its potential applied science, aiding breeders improving thermoresilience. Thermomorphogenesis, developmental response to warm temperatures, has been primarily studied seedlings regulation flowering time. PHYTOCHROME B PHYTOCHROME-INTERACTING FACTORs (PIFs), particularly PIF4, are key components this response. However, thermoresponse other adult vegetative tissues reproductive structures not systematically evaluated, especially concerning involvement phyB PIFs. Results We screened responses wild type several phyB-PIF4 pathway Arabidopsis mutant lines combined integrative phenotyping platforms root growth soil, shoot, inflorescence, seed. Our findings demonstrate that generally involved relay signals throughout including stage. Furthermore, we identified correlative between shoot tissues. This automated was complemented by monitoring changes transcript levels organs. Transcriptomic profiling pistils from grown under elements may provide insight into molecular behind temperature-induced reduced fertilization rate. These include a downregulation auxin metabolism, upregulation genes signalling, miRNA156 miRNA160 pathways, pollen tube attractants. Conclusions interpretation pervasive processes directly linked

Язык: Английский

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Variation in reproductive organ functionality among a population of tomato genotypes reveals the importance of pollen viability and fruit set in response to heat stress

International Journal of Vegetable Science, Год журнала: 2024, Номер unknown, С. 1 - 15

Опубликована: Ноя. 17, 2024

Язык: Английский

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