Less is more: CRISPR/Cas9-based mutations in DND1 gene enhance tomato resistance to powdery mildew with low fitness costs DOI Creative Commons
Ruiling Li,

Lei Cui,

Matteo Martina

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 10, 2024

Powdery mildew (PM), triggered by Oidium neolycopersici, represents a significant threat and major concern for the productivity of tomato plants (Solanum lycopersicum L.). The presence susceptibility (S) genes in facilitates pathogen proliferation their dysfunction can lead to recessively inherited broad-spectrum durable type resistance. Past studies have demonstrated that disrupting function DND1 (Defense No Death 1) increases plant resilience against various pathogens, such as powdery but this comes at cost negatively affecting overall health vigor plant. To investigate possibility minimizing adverse effects dnd1 mutation while boosting disease resistance, CRISPR-Cas9 construct with four single guide RNAs targeting three exons SlDND1 (Solyc02g088560.4.1) was designed introduced into variety Moneymaker (MM) through Agrobacterium tumefaciens-mediated transformation. Three T

Язык: Английский

The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke DOI Creative Commons
Matteo Martina, Aldana Zayas, Ezio Portis

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Май 17, 2024

Abstract Globe artichoke ( Cynara cardunculus var. scolymus ; 2n = 2x 34) is a food crop consumed for its immature flower heads. Traditionally, globe varietal types are vegetatively propagated. However, seed propagation makes it possible to treat the as annual, increasing field uniformity and reducing farmers costs, well pathogens diffusion. Despite artichoke’s significant agricultural value critical role of heterosis in development superior varieties, production hybrids remains challenging without reliable system large-scale industrial production. Male sterility (MS) presents promising avenue overcoming these challenges by simplifying hybridization process enabling cost-effective within genus, genic male has been linked three recessive loci artichoke, with no definitive genetic mechanism elucidated date. A 250 offsprings F 2 population, derived from cross between MS fertile (MF) cultivated cardoon C. altilis ) fitting monogenic segregation model (3:1), was analyzed through BSA-seq, aiming at identification genomic regions/genes affecting sterility. Four QTL regions were identified on chromosomes 4, 12, 14. By analyzing sequence around highest pick chromosome 14, cytochrome P450 CYP703A2 identified, carrying deleterious substitution (R/Q) fixed sterile parent. single dCAPS marker developed this SNP, allowing discrimination MF genotypes suitable applications plant breeding programs. 3D protein generated homology modeling, revealing that mutated amino acid part highly conserved motif crucial folding.

Язык: Английский

Процитировано

1

Less is more: CRISPR/Cas9-based mutations in DND1 gene enhance tomato resistance to powdery mildew with low fitness costs DOI Creative Commons
Ruiling Li,

Lei Cui,

Matteo Martina

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 10, 2024

Powdery mildew (PM), triggered by Oidium neolycopersici, represents a significant threat and major concern for the productivity of tomato plants (Solanum lycopersicum L.). The presence susceptibility (S) genes in facilitates pathogen proliferation their dysfunction can lead to recessively inherited broad-spectrum durable type resistance. Past studies have demonstrated that disrupting function DND1 (Defense No Death 1) increases plant resilience against various pathogens, such as powdery but this comes at cost negatively affecting overall health vigor plant. To investigate possibility minimizing adverse effects dnd1 mutation while boosting disease resistance, CRISPR-Cas9 construct with four single guide RNAs targeting three exons SlDND1 (Solyc02g088560.4.1) was designed introduced into variety Moneymaker (MM) through Agrobacterium tumefaciens-mediated transformation. Three T

Язык: Английский

Процитировано

1