Powdery
mildew
(PM),
triggered
by
Oidium
neolycopersici,
represents
a
significant
threat
and
major
concern
for
the
productivity
of
tomato
plants
(Solanum
lycopersicum
L.).
The
presence
susceptibility
(S)
genes
in
facilitates
pathogen
proliferation
their
dysfunction
can
lead
to
recessively
inherited
broad-spectrum
durable
type
resistance.
Past
studies
have
demonstrated
that
disrupting
function
DND1
(Defense
No
Death
1)
increases
plant
resilience
against
various
pathogens,
such
as
powdery
but
this
comes
at
cost
negatively
affecting
overall
health
vigor
plant.
To
investigate
possibility
minimizing
adverse
effects
dnd1
mutation
while
boosting
disease
resistance,
CRISPR-Cas9
construct
with
four
single
guide
RNAs
targeting
three
exons
SlDND1
(Solyc02g088560.4.1)
was
designed
introduced
into
variety
Moneymaker
(MM)
through
Agrobacterium
tumefaciens-mediated
transformation.
Three
T
Abstract
Globe
artichoke
(
Cynara
cardunculus
var.
scolymus
;
2n
=
2x
34)
is
a
food
crop
consumed
for
its
immature
flower
heads.
Traditionally,
globe
varietal
types
are
vegetatively
propagated.
However,
seed
propagation
makes
it
possible
to
treat
the
as
annual,
increasing
field
uniformity
and
reducing
farmers
costs,
well
pathogens
diffusion.
Despite
artichoke’s
significant
agricultural
value
critical
role
of
heterosis
in
development
superior
varieties,
production
hybrids
remains
challenging
without
reliable
system
large-scale
industrial
production.
Male
sterility
(MS)
presents
promising
avenue
overcoming
these
challenges
by
simplifying
hybridization
process
enabling
cost-effective
within
genus,
genic
male
has
been
linked
three
recessive
loci
artichoke,
with
no
definitive
genetic
mechanism
elucidated
date.
A
250
offsprings
F
2
population,
derived
from
cross
between
MS
fertile
(MF)
cultivated
cardoon
C.
altilis
)
fitting
monogenic
segregation
model
(3:1),
was
analyzed
through
BSA-seq,
aiming
at
identification
genomic
regions/genes
affecting
sterility.
Four
QTL
regions
were
identified
on
chromosomes
4,
12,
14.
By
analyzing
sequence
around
highest
pick
chromosome
14,
cytochrome
P450
CYP703A2
identified,
carrying
deleterious
substitution
(R/Q)
fixed
sterile
parent.
single
dCAPS
marker
developed
this
SNP,
allowing
discrimination
MF
genotypes
suitable
applications
plant
breeding
programs.
3D
protein
generated
homology
modeling,
revealing
that
mutated
amino
acid
part
highly
conserved
motif
crucial
folding.
Powdery
mildew
(PM),
triggered
by
Oidium
neolycopersici,
represents
a
significant
threat
and
major
concern
for
the
productivity
of
tomato
plants
(Solanum
lycopersicum
L.).
The
presence
susceptibility
(S)
genes
in
facilitates
pathogen
proliferation
their
dysfunction
can
lead
to
recessively
inherited
broad-spectrum
durable
type
resistance.
Past
studies
have
demonstrated
that
disrupting
function
DND1
(Defense
No
Death
1)
increases
plant
resilience
against
various
pathogens,
such
as
powdery
but
this
comes
at
cost
negatively
affecting
overall
health
vigor
plant.
To
investigate
possibility
minimizing
adverse
effects
dnd1
mutation
while
boosting
disease
resistance,
CRISPR-Cas9
construct
with
four
single
guide
RNAs
targeting
three
exons
SlDND1
(Solyc02g088560.4.1)
was
designed
introduced
into
variety
Moneymaker
(MM)
through
Agrobacterium
tumefaciens-mediated
transformation.
Three
T
