β-Glucosidase VmGlu1 is required for toxin production and pathogenicity of Valsa mali

Phytopathology Research, Год журнала: 2025, Номер 7(1)

Опубликована: Янв. 23, 2025

Abstract Flavonoids, serving as crucial secondary metabolites, are widely distributed in plants and play a key role inhibiting microbial growth, protecting from pathogen invasion, conserving energy. Our previous study revealed that the β-glucosidase VmGlu2 effectively catalyzes degradation of phloridzin, typical flavonoid apples, regulates pathogenic process Valsa mali . However, exhibits lower efficiency degrading rutin, another characteristic apples. In this study, we found expression level gene VmGlu1 was induced by 9.42-fold following rutin treatment, possessed capability to degrade both but under same conditions, showed distinct preference for degradation, with an initial rate 8.66 times higher than VmGlu2. Targeted deletion resulted 99.32% reduction pycnidia production, nearly 33% decrease toxin significant pathogenicity on apple twigs leaves. Furthermore, verified exhibit functional redundancy toxin-related activity, generating double-deletion mutants. The results provide valuable insights into roles VmGlus interaction V. during infection process.

Язык: Английский

Global characterization of GH11 family xylanases genes in Neostagonosporella sichuanensis and functional analysis of Nsxyn1 and Nsxyn2

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 21, 2024

Rhombic-spot disease, caused mainly by

Язык: Английский