Role of Plant Growth-Promoting Rhizobacteria in Sustainable Agriculture: Addressing Environmental and Biological Challenges

Journal of Plant Physiology, Год журнала: 2025, Номер 307, С. 154455 - 154455

Опубликована: Фев. 20, 2025

Язык: Английский

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Phylogenetic relationship of WRKY transcription factors in Solanum and potato genes in response to hormonal and biotic stresses

Plant Signaling & Behavior, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 11, 2025

WRKY transcription factors are important regulators of plant responses to environmental stresses and hormone signaling. This study analyzes the gene family in Solanum tuberosum by examining phylogenetic relationships, expression profiles, their roles abiotic stress responses. Phylogenetic tree was constructed using 322 genes from four species: S. tuberosum, pennellii, pimpinellifolium, lycopersicum. The results revealed conserved expanded across these species. We then studied 75 SotuWRKY response salt, drought, heat stresses, treatments (IAA, ABA, BABA, GA3, BAP). Results showed that 19, 25, 29 were regulated under respectively. Several (e.g. SotuWRKY03 SotuWRKY24) also biotic like Phytophthora infestans infection treatments, indicating involvement defense mechanisms. A co-expression network based on gene-to-gene correlations, where SotuWRKY52 identified as a hub gene, positively regulating six negatively four. These findings suggest potato play key signaling, potentially enhancing resistance diseases. provides new insights into other

Язык: Английский

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Modes of Action of Biocontrol Agents from Rhizosphere to Phyllosphere Signaling

Опубликована: Янв. 1, 2025

Язык: Английский

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Heterologous expression of the barley-specific HvbZIP87 transcription factor in wheat enhances broad-spectrum disease resistance with balanced yield

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

NPR1, a protein that interacts with bZIP transcription factors known as TGAs, plays pivotal role in coordinating systemic acquired resistance (SAR) plants. Nevertheless, the molecular intricacies governing SAR Triticeae family, which includes crops like wheat and barley, are still largely enigmatic. We identified HvbZIP87, barley-specific factor, induced SAR, more strongly transgenic barley HvNPR1 knocked down. The objective of this research is to explore HvbZIP87 defense mechanisms plants, focusing on lines have been engineered overexpress (HvbZIP87-OE). Initially, broad-spectrum disease levels HvbZIP87-OE were evaluated. Multiple techniques employed validate direct interaction between NPR1. RNA-seq DAP-seq performed analyze gene regulatory effects lines. Transgenic expressing exhibited significantly enhanced improved plant stripe rust, leaf spot blotch, Fusarium crown rot. Despite some adverse agronomic traits, heterologous expression resulted balanced yield due larger harvested seeds. Intriguingly, physically interacted TaNPR1 cell nucleus. Transcriptome sequencing revealed networks cis-elements governed by genome. genes TaPR1, TaPR2, TaPR4, TaPR5, among several PR genes, forecasted undergo regulation HvbZIP87. Additionally, we TaMYC2 factor another interactor Silencing further wheat's suggesting its negative defense. unique pathway species clarified conferring resistance.

Язык: Английский

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Activation of the phenylpropanoid pathway and enhanced powdery mildew resistance in honeysuckle induced by inactivated Erysiphe lonicerae spores

Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102739 - 102739

Опубликована: Май 1, 2025

Язык: Английский

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Wheat TaNADPO promotes spot blotch resistance

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 19, 2024

ABSTRACT Bipolaris sorokiniana is a common soil-borne fungal pathogen that can infect various organs of wheat ( Triticum aestivum L.), causing diseases such as spot blotch, root rot, head blight, and black point. The genetic basis resistance to B. not yet fully understood. In this study, natural population 1,302 global germplasms was established inoculated with at the seedling stage in greenhouse. Resistance blotch assessed, revealing only about 3.8% exhibited moderate or higher levels. A genome-wide association study (GWAS) using high-density 660K single nucleotide polymorphism (SNP) data identified region on chromosome 1BL (621.2-674.0 Mb) 9 SNPs significantly associated p < 10e-4) resistance, designated Qsb.hebau-1BL . RNA sequencing qRT-PCR assays showed gene TraesCS1B02G410300 , encoding nicotinamide-adenine dinucleotide phosphate-binding oxidoreductase (TaNADPO), induced by Five SNP variations were found promoter TaNADPO lines without Transient expression Nicotiana benthamiana leaves cytoplasmic subcellular localization fusion protein green fluorescent (GFP) tag. Wheat transgenic overexpressing enhanced compared wildtype plants, accumulation reactive oxygen species (ROS). knockout EMS mutant turgidum NADPO tdnadpo-K2561 Gln125*) reduced lower ROS plants. summary, has been crucial for providing valuable insights developing blotch-resistant varieties through molecular breeding techniques.

Язык: Английский

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Overexpressing CsSABP2 enhances tolerance to Huanglongbing and citrus canker in C. sinensis

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Окт. 8, 2024

Huanglongbing (HLB) and citrus canker, arising from Candidatus Liberibacter asiaticus ( Ca Las) Xanthomonas citri pv. Citri Xcc ), respectively, have been imposing tremendous losses to the global industry. Systemic acquired resistance (SAR) has shown be crucial for priming defense against pathogen in citrus. Salicylic acid (SA) binding protein 2 (SABP2), which is responsible converting methyl salicylate (MeSA) SA, essential full SAR establishment. Here, we characterized functions of four SABP2 genes CsSABP2-1 , V18A CsSABP2-2 CsSABP2-3 ) HLB canker. In vitro enzymatic assay revealed that all proteins had MeSA esterase activities, strongest activity. Their activities were inhibited by SA except . Four controlled a strong promoter 35S induced into Wanjincheng orange Citrus sinensis Osbeck) generate transgenic plants overexpressing CsSABP2 Overexpressing increased content action on SA. Resistance evaluation demonstrated only significantly enhanced tolerance HLB, although CsSABP2s The data suggested amino Val-18 active site plays key role function. Our study emphasized balancing levels regulating conferring broad-spectrum This finding offers valuable insights enhancing through engineering.

Язык: Английский

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Immunomodulating melatonin-decorated silica nanoparticles suppress bacterial wilt (Ralstonia solanacearum) in tomato (Solanum lycopersicum L.) through fine-tuning of oxidative signaling and rhizosphere bacterial community

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 12, 2024

Tomato (Solanum lycopersicum L.) production is severely threatened by bacterial wilt, caused the phytopathogenic bacterium Ralstonia solanacearum. Recently, nano-enabled strategies have shown tremendous potential in crop disease management. This study investigates efficacy of biogenic nanoformulations (BNFs), comprising silica nanoparticles (SiNPs) and melatonin (MT), controlling wilt tomato. SiNPs were synthesized using Zizania latifolia leaves extract. Further, MT containing BNFs through one-pot approach. Nanomaterials characterized standard characterization techniques. Greenhouse assays conducted to assess impact on tomato plant immunity resistance wilt. The exhibited a spherical morphology, with particle sizes ranging from 13.02 nm 22.33 for 17.63 21.79 BNFs, indicating relatively uniform size distribution consistent shape across both materials. experiments revealed that soil application outperformed SiNPs, significantly enhancing reducing incidence 78.29% plants maintaining oxidative stress homeostasis via increasing activities antioxidant enzymes such as superoxide dismutase (31.81%), peroxidase (32.9%), catalase (32.65%), ascorbate (47.37%) compared untreated infected plants. Additionally, induced salicylic acid activating defense-related genes (e.g., SlPAL1, SlICS1, SlNPR1, SlEDS, SlPD4, SlSARD1) involved phytohormones signaling High-throughput 16 S rRNA sequencing promoted growth beneficial rhizosphere bacteria (Gemmatimonadaceae, Ramlibacter, Microscillaceae, Anaerolineaceae, Chloroplast Phormidium) healthy diseased plants, while suppressing R. solanacearum abundance Overall, these findings suggest offer more promising sustainable approach managing

Язык: Английский

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DNA methylation in wheat: current understanding and future potential for enhancing biotic and abiotic stress tolerance

Physiology and Molecular Biology of Plants, Год журнала: 2024, Номер 30(12), С. 1921 - 1933

Опубликована: Дек. 1, 2024

Язык: Английский

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Dufulin Impacts Plant Defense Against Tomato Yellow Leaf Curl Virus Infecting Tomato

Viruses, Год журнала: 2024, Номер 17(1), С. 53 - 53

Опубликована: Дек. 31, 2024

Tomato yellow leaf curl virus (TYLCV) poses a significant threat to tomato production, leading severe yield losses. The current control strategies primarily rely on the use of pesticides, which are often nonselective and costly. Therefore, there is an urgent need identify more environmentally friendly alternatives. Dufulin, novel compound that has been effective in controlling viral diseases tobacco rice, not yet tested against TYLCV. This study assessed efficacy dufulin TYLCV over three-year span from 2021 2023 through field trials, by monitoring disease symptoms titers. Additionally, this expression levels genes associated with systemic acquired resistance (SAR), specifically proteinase inhibitor II (PI II) non-expressor pathogenesis-related 1 (NPR1), using real-time qRT-PCR. chlorophyll nitrogen content leaves were also measured. Plants treated showed reduced symptomatology lower titers compared controls. Analysis gene revealed NPR1 was upregulated dufulin-treated plants, whereas PI consistently downregulated TYLCV-infected plants. Interestingly, increased healthy plants following seven-day post-treatment dufulin. Moreover, exhibited higher than controls, though no differences observed between water-treated Overall, application significantly bolstered plant’s defense response, effectively reducing enhancing resistance.

Язык: Английский

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