Lignin accumulation in cell wall plays a role in clubroot resistance DOI Creative Commons
J. C. Tu, Qin Li,

Chithra Karunakaran

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июль 23, 2024

Clubroot, caused by Plasmodiophora brassicae , is a significant disease affecting brassica crops worldwide and poses threat to canola ( Brassica napus ) production in western Canada. Management of this heavily relies on the use resistant cultivars, but resistance erosion serious concern due highly diverse pathogen populations. Understanding mechanisms may aid better deployment/rotation clubroot (CR) genes improve resilience. In study, we conducted comparative analysis using varieties carrying either single Rcr1 or double CR + Crr1 rutb decipher modes associated with these genes. Cell wall (CW) biopolymeric compounds different root layers were mapped quantified Fourier-transform mid-infrared microspectroscopy for changes CW elements resistance. Transmission electron confocal microscopy used assess infection details relative transcript abundance was analyzed determine activation lignin-related pathway relation Neither variety affected primary hairs/epidermal cells compared susceptible “Westar”, both exhibited strong inhibition cortical infection, effectively ‘trapping’ exodermis. The most prominent change observed increased lignin accumulation Westar, able degrade lignin, facilitating access cortex secondary plasmodia P . contrast, showed clear around penetration site exodermis, accompanied elevated expression involved phenylpropanoid pathway. These results suggest that induced plays role mediated canola, providing cellular structural evidence supports data from earlier transcriptomic studies.

Язык: Английский

Transcriptome and nutritional composition analysis of stacked transgenic maize with insect resistance and herbicide tolerance DOI Creative Commons
Xiaoxing Yu, Hongyu Gao, Pengfei Wang

и другие.

GM crops & food, Год журнала: 2025, Номер 16(1), С. 216 - 234

Опубликована: Фев. 27, 2025

The safety assessment of stacked transgenic crops is essential for their commercial cultivation. A crucial element the nutritional evaluation crops. Currently, profiling methods like transcriptome are employed as supplemental analytical tools to find unintended effects In this study, maize ZDRF8×nCX-1 was produced by crossing two events ZDRF8 and nCX-1. This expresses five genes: cry1Ab, cry2Ab g10evo-epsps (from ZDRF8), well cp4 epsps P450-N-Z1 nCX-1). Molecular analysis showed that insertion sites target genes were not changed during stack breeding, effectively expressed at both RNA protein levels in ZDRF8×nCX-1. Target trait exhibits tolerant glyphosate, flazasulfuron MCPA, resistant damage corn borers. Transcriptome revealed gene-stacked did significantly alter profiles compared Nutritional composition grain profile substantially equivalent non-transgenic counterpart. These results suggest hybrid stacking does cause beyond providing intended beneficial traits.

Язык: Английский

Процитировано

0
Clubroot Resistant in Cruciferous Crops: Recent Advances in Genes and QTLs Identification and Utilization DOI Creative Commons

Shangxiang Lai,

Yunshuai Huang,

Yumei Liu

и другие.

Horticulture Research, Год журнала: 2025, Номер 12(7)

Опубликована: Апрель 16, 2025

Язык: Английский

Процитировано

0
Lignin accumulation in cell wall plays a role in clubroot resistance DOI Creative Commons
J. C. Tu, Qin Li,

Chithra Karunakaran

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июль 23, 2024

Clubroot, caused by Plasmodiophora brassicae , is a significant disease affecting brassica crops worldwide and poses threat to canola ( Brassica napus ) production in western Canada. Management of this heavily relies on the use resistant cultivars, but resistance erosion serious concern due highly diverse pathogen populations. Understanding mechanisms may aid better deployment/rotation clubroot (CR) genes improve resilience. In study, we conducted comparative analysis using varieties carrying either single Rcr1 or double CR + Crr1 rutb decipher modes associated with these genes. Cell wall (CW) biopolymeric compounds different root layers were mapped quantified Fourier-transform mid-infrared microspectroscopy for changes CW elements resistance. Transmission electron confocal microscopy used assess infection details relative transcript abundance was analyzed determine activation lignin-related pathway relation Neither variety affected primary hairs/epidermal cells compared susceptible “Westar”, both exhibited strong inhibition cortical infection, effectively ‘trapping’ exodermis. The most prominent change observed increased lignin accumulation Westar, able degrade lignin, facilitating access cortex secondary plasmodia P . contrast, showed clear around penetration site exodermis, accompanied elevated expression involved phenylpropanoid pathway. These results suggest that induced plays role mediated canola, providing cellular structural evidence supports data from earlier transcriptomic studies.

Язык: Английский

Процитировано

2