
Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Июль 23, 2024
Clubroot, caused by Plasmodiophora brassicae , is a significant disease affecting brassica crops worldwide and poses threat to canola ( Brassica napus ) production in western Canada. Management of this heavily relies on the use resistant cultivars, but resistance erosion serious concern due highly diverse pathogen populations. Understanding mechanisms may aid better deployment/rotation clubroot (CR) genes improve resilience. In study, we conducted comparative analysis using varieties carrying either single Rcr1 or double CR + Crr1 rutb decipher modes associated with these genes. Cell wall (CW) biopolymeric compounds different root layers were mapped quantified Fourier-transform mid-infrared microspectroscopy for changes CW elements resistance. Transmission electron confocal microscopy used assess infection details relative transcript abundance was analyzed determine activation lignin-related pathway relation Neither variety affected primary hairs/epidermal cells compared susceptible “Westar”, both exhibited strong inhibition cortical infection, effectively ‘trapping’ exodermis. The most prominent change observed increased lignin accumulation Westar, able degrade lignin, facilitating access cortex secondary plasmodia P . contrast, showed clear around penetration site exodermis, accompanied elevated expression involved phenylpropanoid pathway. These results suggest that induced plays role mediated canola, providing cellular structural evidence supports data from earlier transcriptomic studies.
Язык: Английский