Complex interplay: The interactions between citrus tristeza virus and its host

Virology, Год журнала: 2025, Номер 603, С. 110388 - 110388

Опубликована: Янв. 2, 2025

Язык: Английский

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An Agrobacterium‐mediated base editing approach generates transgene‐free edited banana

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Genome editing for the development of improved varieties is supported by possibility segregating out editor T-DNA cassette after genome in many crop species. Removal prevents potential continuous activity transformed plant and furthermore facilitates regulatory approval. While transgene outcrossing exogenous sequences possible crops, this not case vegetatively propagated sterile such as Cavendish bananas. Therefore, gene techniques leading to transgene-free edited plants are essential untap those crops. Here, we present a method banana (Musa spp.) through co-editing strategy. A novel Agrobacterium tumefaciens-mediated approach combining embryogenesis chlorsulfuron selection was established validated whole sequencing. Editing acetolactate synthase (MaALS) genes using base allows effective plants. Moreover, plantlets were regenerated with mutations at two target sites, indicating that strategy can be used multiple genomic sites. The presented efficient represents first report

Язык: Английский

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Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Авг. 1, 2024

Citrus canker, caused by

Язык: Английский

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Development and application of gene editing in citrus: how and what to do

Horticulture Advances, Год журнала: 2024, Номер 2(1)

Опубликована: Дек. 10, 2024

Abstract Conventional breeding techniques have been effectively utilized for the enhancement of citrus varieties. Nonetheless, traits such as an extended juvenile phase, cross- or self-incompatibility, high genetic heterozygosity, and polyembryony posed significant challenges limitations to these methods. The clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has progressively emerged a vital tool research. This article reviews array CRISPR/Cas systems, emphasizes recent advancements in using CRISPR/Cas, explores application this technology bolster resistance canker. review also covers development CRISPR/Cas-mediated transformation regeneration systems citrus, alongside approaches generating transgene-free germplasm. Moreover, regulatory landscape societal acceptance are examined. Lastly, potential applications proposed, with attention prospective challenges.

Язык: Английский

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Cas12a/3 crRNAs RNP transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome in the T0 generation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 17, 2024

Abstract Citrus canker, caused by Xanthomonas citri subsp. ( Xcc ), is a devastating disease worldwide. Previously, we successfully generated canker-resistant sinensis cv. Hamlin lines in the T0 generation, achieving mutation efficiency of 97.4%. This was achieved through transformation embryogenic protoplasts using Cas12a/1 crRNA ribonucleoprotein (RNP) system to edit canker susceptibility gene, CsLOB1 , which led small indels. Here, transformed with Cas12a/3 crRNAs RNP, resulting 100% editing gene generation. Among 10 transgene-free genome-edited lines, long deletions were obtained five lines. Additionally, inversions observed three edited deletions, but not any short indel mutations, suggesting are required for inversions. Biallelic mutations each target sites 4 when 3 used, demonstrating that citrus RNP can be very efficient multiplex editing. Our analysis revealed absence off-target These cslob1 mutant and no symptoms after inoculation growth significantly reduced compared wild type plants. Taken together, provides promising solution genome high deletion fragments.

Язык: Английский

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A novel and efficient <i>Agrobacterium</i>-mediated transient gene expression in citrus epicotyls and mature stem tissues

Fruit Research, Год журнала: 2020, Номер 0(0), С. 1 - 10

Опубликована: Янв. 1, 2020

Язык: Английский

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