Biological Decline of Alfalfa Is Accompanied by Negative Succession of Rhizosphere Soil Microbial Communities
Yuanyuan Ma,
Yan Shen,
Xiaoping Zhou
и другие.
Plants,
Год журнала:
2024,
Номер
13(18), С. 2589 - 2589
Опубликована: Сен. 16, 2024
The
growth
and
biological
decline
of
alfalfa
may
be
linked
to
the
rhizosphere
microbiome.
However,
plant–microbe
interactions
in
associated
microbial
community
variations
with
stand
age
remain
elusive.
This
study
explored
successional
pattern
communities
across
different
aged
stands
its
relationship
decline.
Rhizosphere
soils
were
collected
from
2-
6-year-old
stands.
Control
interspaces
between
plants
same
Soil
bacterial
fungal
characterized
by
16S
ITS
rRNA
gene
sequencing,
respectively.
Specific
taxa
colonized
soils,
but
not
control
soils.
rhizosphere-specific
mainly
included
potentially
beneficial
genera
(e.g.,
Dechloromonas,
Verrucomicrobium)
young
harmful
Peziza,
Campylocarpon)
old
stand.
Alfalfa
roots
regulated
soil
selective
promotion
or
inhibition
distinct
taxa.
majority
time-enriched
reported
as
fungi,
whose
relative
abundances
negatively
correlated
plant
traits.
Time-depleted
mostly
known
bacteria,
which
had
positively
functional
genes
vancomycin
biosynthesis,
zeatin
amino
acid
metabolism
trended
lower
An
upward
trend
was
observed
for
pathogens
wood
saprotrophs
increasing
age.
results
suggest
that
root
activity
drives
negative
succession
during
Язык: Английский
Endophytic strategies decoded by genome and transcriptome analysis of Fusarium nematophilum strain NQ8GII4
Frontiers in Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 15, 2025
Fusarium
nematophilum
strain
NQ8GII4
is
an
endophytic
fungus
with
significant
potential
for
improving
growth
and
disease
resistance
of
alfalfa.
However,
the
molecular
mechanisms
underlying
symbiotic
relationship
between
alfalfa
roots
remain
poorly
understood.
In
this
study,
we
conducted
(1)
a
comparative
genomic
analysis
selected
saprophytic,
pathogenic,
fungi,
including
phylogeny
analysis,
whole-genome
alignment,
divergence
date
estimation
positioning,
(2)
transcriptomic
profiling
infected
NQ8GII4.
Our
findings
reveal
that
genetically
closely
related
to
F.
solani,
suggesting
it
diverged
from
phytopathogens.
During
early
stages
symbiosis
establishment,
genes
encoding
glycosyltransferases
(GTs),
fungal
cell
wall-degrading
enzymes
(FCWDEs),
steroid-14α-demethylase
(CYP51)
were
significantly
downregulated,
potentially
suppressing
hyphal
fungus.
Once
was
established,
secreted
effectors
activated
plant
immunity,
which
in
turn
could
slow
Moreover,
involved
secondary
metabolite
biosynthesis,
such
as
type
I
polyketide
synthases
(T1PKS)
non-ribosomal
peptide
synthetases
(NRPSs),
downregulated.
Homologs
autophagy-related
genes,
ATG1,
ATG2,
ATG11,
others,
also
reduced
phytotoxin
production
autophagy
inhibition
consequence
NQ8GII4's
symbiosis.
This
study
investigated
comprehensive
genetic
governing
interaction
roots.
Beyond
NQ8GII4-alfalfa
system,
these
provide
valuable
framework
understanding
mechanism
interactions
fungi
their
host
plants.
Язык: Английский
First report of Fusarium acuminatum from traditional-saffron region of India: Evidence of Corm Rot pathogenicity in Crocus sativus L.
Physiological and Molecular Plant Pathology,
Год журнала:
2025,
Номер
unknown, С. 102702 - 102702
Опубликована: Апрель 1, 2025
Язык: Английский
Molecular, Morphological Identification, and Virulence Profiling of Fusarium Oxysporum F.Sp. Lycopersici (Sacc.) W.C. Snyder & H.N. Hansen Inciting Fusarium Wilt of Tomato (Solanum Lycopersicum L.)
Опубликована: Янв. 1, 2025
Язык: Английский
Molecular, Morphological identification, and Virulence profiling of Fusarium oxysporum f.sp. lycopersici (Sacc.) (W.C. Snyder & H.N. Hansen) associated with Root- knot Nematode inciting Fusarium wilt of tomato
Physiological and Molecular Plant Pathology,
Год журнала:
2025,
Номер
unknown, С. 102741 - 102741
Опубликована: Май 1, 2025
Язык: Английский
DNA Polymerase Theta Regulates the Growth and Development of Fusarium acuminatum and Its Virulence on Alfalfa
Agriculture,
Год журнала:
2025,
Номер
15(11), С. 1128 - 1128
Опубликована: Май 23, 2025
Fusarium
acuminatum
is
a
major
pathogenic
fungus
causing
root
rot
in
alfalfa
(Medicago
sativa).
DNA
polymerase
theta
known
to
play
crucial
role
repairing
double-strand
breaks.
However,
its
biological
function
F.
remains
unknown.
In
this
study,
the
POLQ
gene
was
deleted
by
homologous
recombination
using
Agrobacterium
tumefaciens-mediated
transformation.
Compared
wild
type
(with
gene),
mutants
(without
gene)
showed
significant
phenotypic
changes:
they
produced
brown-yellow
pigments
instead
of
pink,
slowed
mycelial
growth,
and
exhibited
changes
macroconidia
size
shape.
The
virulence
greatly
reduced,
inducing
only
mild
symptoms
alfalfa.
addition,
FITC-WGA
staining
impaired
spore
germination
hyphal
growth.
These
results
suggest
that
key
regulating
growth
development
acuminatum,
indicating
repair
may
an
essential
pathogenicity
pathogen
could
thus
be
promising
target
for
limiting
infections
Язык: Английский
Cultivable Rhizosphere Microbial Community Structure in the Yaqui Valley’s Agroecosystems
Soil Systems,
Год журнала:
2024,
Номер
8(4), С. 112 - 112
Опубликована: Окт. 31, 2024
Agricultural
practices
affect
the
physical,
chemical,
and
biological
properties
of
soil
in
agroecosystems.
This
study
evaluated
impact
food
production
strategies
on
rhizosphere
microbial
communities
Yaqui
Valley,
Mexico,
key
crops
(Medicago
sativa,
Brassica
oleracea,
Asparagus
officinalis,
Phaseolus
vulgaris,
Citrus
sinensis,
Zea
mays,
Solanum
tuberosum,
Triticum
durum,
an
undisturbed
native
ecosystem).
Soil
samples
were
collected
from
30
cm
depths
across
one-hectare
fields
analyzed
for
bulk
density,
pH,
organic
matter
content,
electrical
conductivity.
Standardized
methods
used
these
analyses,
along
with
isolation
through
culturing,
PCR
amplification,
DNA
sequencing
identification.
The
use
synthetic
fertilizers
region
was
linked
to
increased
salinity
compaction.
Organic
content
notably
low
at
≤1.4%,
which
negatively
impacted
diversity.
A
total
317
strains
isolated,
bacteria
comprising
73%
fungi
27%.
Bacillus
most
dominant
bacterial
genus
(41%
isolates),
while
Aspergillus
abundant
fungal
(31%
isolates).
Crop-specific
identified.
provides
first
detailed
insight
into
how
agricultural
shape
Valley’s
major
crops,
highlighting
link
between
Язык: Английский