Widely Targeted Metabolomics and Transcriptomics Analysis of the Response and Adaptation Mechanisms of Trifolium ambiguum to Low-Temperature Stress
Agronomy,
Год журнала:
2025,
Номер
15(2), С. 308 - 308
Опубликована: Янв. 26, 2025
Caucasian
clover
(Trifolium
ambiguum
M.Bieb.)
is
a
perennial
legume
known
for
its
exceptional
cold
tolerance,
commonly
used
in
agriculture
and
ecosystems
climates.
Given
the
impact
of
climate
change,
enhancing
adaptation
crucial
sustaining
agricultural
productivity.
This
study
employs
metabolomics,
transcriptomics,
Weighted
Gene
Co-expression
Network
Analysis
(WGCNA)
to
investigate
molecular
mechanisms
clover’s
response
low-temperature
stress.
Metabolomic
analysis
showed
that
stress
triggered
accumulation
fatty
acids,
amino
antioxidants,
which
are
critical
maintaining
membrane
stability
antioxidant
capacity,
thus
protecting
plant
from
oxidative
damage.
Transcriptomic
revealed
significant
upregulation
genes
involved
adaptation,
particularly
those
related
defense,
lipid
repair,
signal
transduction,
including
ABA
signaling
pathway
enzymes,
thereby
improving
tolerance.
WGCNA
identified
gene
modules
closely
linked
especially
acid
metabolism,
repair.
These
function
synergistically,
with
coordinated
expression
resistance.
also
investigated
isoflavonoid
biosynthesis
under
stress,
highlighting
role
capacity
Low-temperature
induced
key
enzyme
genes,
such
as
Isoflavone
Synthase
(IFS)
Isoflavone-7-O-Glucosyltransferase
(IF7GT),
promoting
metabolite
further
plant’s
adaptation.
Overall,
this
offers
novel
insights
into
tolerance
provides
valuable
theoretical
support
breeding
cold-resistant
crops
Язык: Английский
Integrative Profiling of Phytohormones, Metabolomics, and Transcriptomics Reveals Key Regulators of Cold Tolerance in Cucumber Leaves
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(3)
Опубликована: Март 1, 2025
A
low-temperature
condition
in
a
root
zone
is
major
abiotic
stress
that
threatens
cucumber
(Cucumis
sativus
L.)
growth
and
development,
yet
the
molecular
mechanism
by
which
leaf
reacts
to
chilling
remains
largely
unknown.
In
this
study,
we
applied
three
temperature
treatments,
including
room
(20°C-22°C),
suboptimal
(13°C-15°C),
low
(8°C-10°C),
investigate
how
affects
hormone
dynamics,
metabolomics,
transcriptomics
leaves
of
variety
"Jinyou
35",
main
cultivar
northwest
southwest
China.
Through
integrative
physiological
biochemical
analysis,
auxin
emerges
as
most
significant
accumulated
hormone,
accounting
for
88%
temperature-treated
(RL),
99%
(SL),
94%
low-temperature-treated
(LL).
Under
stress,
flavanones
were
abundant
metabolite
leaves,
constituting
over
50%
total
metabolites,
while
phenolic
acids
showed
marked
decrease.
Several
differentially
expressed
transcription
factors
(DETFs),
such
LOB
(CsaV3_3G020650),
MYB
(CsaV3_3G043510),
bHLH
(CsaV3_2G005070
CsaV3_4G029740),
upregulated
SL
LL,
potentially
enhancing
cucumber's
defense
against
injury.
Additionally,
terminal
flower
formation
was
observed
under
conditions,
with
CsFT
expression
LL
lower
than
RL,
negative
correlation
between
CsNAC6.
These
findings
deepen
our
understanding
resilience
mechanisms
shedding
light
on
its
cold
tolerance
strategies.
Язык: Английский
Transcriptome and metabolome analyses reveal the potential mechanism of root zone temperature regulates the growth rhythm of switchgrass (Panicum virgatum L.) seedlings
Industrial Crops and Products,
Год журнала:
2025,
Номер
230, С. 121041 - 121041
Опубликована: Апрель 29, 2025
Язык: Английский