Integrative analysis of transcriptome and metabolome reveals molecular mechanisms of dynamic change of storage substances during dehydration and drying process in peanuts (Arachis hypogaea L.) DOI Creative Commons
Jingjing Deng, Mingyu Hou,

Shunli Cui

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Апрель 16, 2025

Various substances in seeds occurred many transformations during the drying process, which is key to long-term storage, but mechanism unclear. In this study, of peanut (Arachis hypogaea L.) variety "Silihong" were selected as experimental materials. Transcriptome and metabolome analyses kernels at day 0 (S0d), 1 (S1d), 3 (S3d), 5 (S5d), 7 (S7d) performed search for genes that controlled storage compounds. A total 165 differentially expressed metabolites (DAMs) 15,010 (DEGs) five stages seed identified. S3d was period content most changed significantly. The contents amino acids(87%) their derivatives decreased significantly, lipids(68%), sugars(67%) flavonoids(87%) accumulated peak S3d. Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis revealed DEGs primarily enriched four aspects, including acid biosynthesis metabolism, lipid sucrose starch flavonoid biosynthesis. Crucial potentially regulate identified, PAL, FAD2, SUS, LOX, PFK. Overall, study provides valuable insights into molecular regulation compounds may help assess edible peanuts have enhanced nutritional economic values.

Язык: Английский

Metabolomic Analysis of Maize Response to Northern Corn Leaf Blight DOI Creative Commons

Yingnan Gu,

Bowei Yan, Ye Yang

и другие.

Metabolites, Год журнала: 2025, Номер 15(2), С. 113 - 113

Опубликована: Фев. 10, 2025

Background: As a major food crop, maize is highly susceptible to pathogenic bacteria, which greatly reduces its yield and quality. Metabolomics reveals physiological biochemical changes in organisms aids analyzing metabolic caused by various factors. Methods: This study utilized metabolomics examine maize’s after NCLB infestation, aiming uncover related pathways potential biomarkers. The metabolite measurements were performed during the silking stage. Results: PCA showed an obvious dispersion between treated untreated groups. OPLS-DA identified 1274 differential metabolites, with 242 being downregulated (mainly phenolics esters) 1032 upregulated (primarily organic acids, amino sugars, derivatives). KEGG annotation revealed 50 affected pathways, biosynthesis of secondary metab-olites acids was significantly enriched. Conclusions: We hypothesized that sugar metabolism, proline jasmonic acid synthesis are associated susceptibility. These findings provide critical insights into responses biotic stress, offering theoretical basis for future research on plant resistance mechanisms.

Язык: Английский

Процитировано

0

Integrative analysis of transcriptome and metabolome reveals molecular mechanisms of dynamic change of storage substances during dehydration and drying process in peanuts (Arachis hypogaea L.) DOI Creative Commons
Jingjing Deng, Mingyu Hou,

Shunli Cui

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Апрель 16, 2025

Various substances in seeds occurred many transformations during the drying process, which is key to long-term storage, but mechanism unclear. In this study, of peanut (Arachis hypogaea L.) variety "Silihong" were selected as experimental materials. Transcriptome and metabolome analyses kernels at day 0 (S0d), 1 (S1d), 3 (S3d), 5 (S5d), 7 (S7d) performed search for genes that controlled storage compounds. A total 165 differentially expressed metabolites (DAMs) 15,010 (DEGs) five stages seed identified. S3d was period content most changed significantly. The contents amino acids(87%) their derivatives decreased significantly, lipids(68%), sugars(67%) flavonoids(87%) accumulated peak S3d. Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis revealed DEGs primarily enriched four aspects, including acid biosynthesis metabolism, lipid sucrose starch flavonoid biosynthesis. Crucial potentially regulate identified, PAL, FAD2, SUS, LOX, PFK. Overall, study provides valuable insights into molecular regulation compounds may help assess edible peanuts have enhanced nutritional economic values.

Язык: Английский

Процитировано

0