Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Май 6, 2025
Abstract
Lettuce
(
Lactuca
sativa
L.),
a
widely
cultivated
leafy
green,
is
valued
for
its
rich
content
of
bioactive
compounds,
including
folates,
vitamins,
tocopherols,
ascorbic
acid,
and
antioxidants.
This
study
aimed
to
evaluate
the
effects
amino
acid
supplementation
on
growth
nutrient
hydroponically
grown
lettuce.
A
greenhouse
experiment
using
completely
randomized
design
(CRD)
was
conducted,
with
three
replications
plants
per
replication.
There
were
4
treatments
(T
0
(Control),
T
1
(Methionine
20
mg/L),
2
(Tryptophan
220
mg/L,
3
(Glycine
200
mg/L)
this
Growth
parameters,
biomass,
leaf
length,
width,
area,
measured
four
weeks
after
transplantation.
L-methionine
resulted
in
significant
improvement
plant
growth,
23.60%
increase
biomass
31.41%
area.
Conversely,
L-tryptophan
treatment
led
substantial
reductions
98.78%
decrease
biomass.
Nutrient
analysis
revealed
that
treatments,
especially
methionine,
enhanced
nitrogen,
phosphorus,
potassium
tissues.
These
results
suggest
has
positive
effect
both
uptake
hydroponic
lettuce,
while
L-glycine
negatively
affect
development.
The
differential
responses
may
be
attributed
their
distinct
roles
metabolism,
methionine
enhancing
sulfur-containing
compounds
proteins
essential
tryptophan
glycine
could
disrupt
metabolic
pathways.
Future
research
should
explore
mechanisms
underlying
these
optimal
concentrations
maximizing
lettuce
production
density.
Modern
sequencing
technologies
have
transformed
the
identification
of
medicinal
plant
species
and
varieties,
overcoming
limitations
traditional
morphological
chemical
approaches.
This
review
explores
key
DNA-based
techniques,
including
molecular
markers,
DNA
barcoding,
high-throughput
sequencing,
their
contributions
to
enhancing
accuracy
reliability
identification.
Additionally,
integration
multi-omics
approaches
is
examined
provide
a
comprehensive
understanding
identity.
The
literature
search
for
this
was
conducted
across
databases
such
as
Google
Scholar,
Web
Science,
PubMed,
using
keywords
related
taxonomy,
genomics,
biotechnology.
Inclusion
criteria
focused
on
peer-reviewed
studies
closely
methods
techniques
that
contribute
significantly
field.
highlights
while
offer
substantial
improvements,
challenges
high
costs,
technical
expertise,
lack
standardized
protocols
remain
barriers
widespread
adoption.
Potential
solutions,
AI-driven
data
analysis
portable
sequencers,
are
discussed.
provides
overview
transformative
impact,
future
perspectives
more
accurate
efficient
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 27, 2025
Introduction
Wheat
leaf
rust
is
one
of
the
most
significant
diseases
affecting
wheat.
With
increase
global
average
temperature,
areas
where
wheat
can
survive
winter
and
persist
through
summer
have
been
expanding.
And
high-temperature
resistant
isolates
were
identified
within
natural
population.
Methods
In
this
study,
two
strains
temperature-sensitive
selected
from
41
collected
Shanxi
Province
between
2021
2022.
The
analysis
disease
severity
differences
among
four
at
different
temperatures
revealed
that
26°C
(
p
<
0.05).
Therefore
was
as
optimal
temperature
for
stress.
Subsequently,
histopathological
observations
conducted
on
Pt
strains,
expression
levels
five
genes
determined,
including
PtHsp90-1
,
PtHp
PtHspHSS1
PtHsp60
PtHspSSB
.
Results
Histopathological
showed
compared
with
number
haustorial
mother
cells
haustoria
not
significantly
affected
by
stress
However,
hyphal
branches
less
affected,
indicating
formation
key
stages
in
resistance
to
heat
Further
reached
their
maximum
6
hpi
under
results
indicated
HSP
play
a
crucial
role
during
germination
stage
urediospores.
Discussion
specific
molecular
mechanism
required
further
study
verification.
conclusion,
early
urediospores
(0–12
h)
(12–24
resisting
rust.
Genes,
Год журнала:
2025,
Номер
16(4), С. 424 - 424
Опубликована: Март 31, 2025
Background/Objectives:
Long
non-coding
RNAs
(lncRNAs)
play
a
crucial
regulatory
role
in
plant
trait
expression
and
disease
management,
making
their
accurate
prediction
key
research
focus
for
guiding
biological
experiments.
While
extensive
studies
have
been
conducted
on
animals
humans,
lncRNA
remains
relatively
limited
due
to
various
challenges,
such
as
data
scarcity
genomic
complexity.
This
study
aims
bridge
this
gap
by
developing
an
effective
computational
method
predicting
lncRNAs,
specifically
classifying
transcribed
RNA
sequences
lncRNAs
or
mRNAs
using
multi-feature
analysis.
Methods:
We
propose
the
multi-feature-fusion
ensemble
learning
(LMFE)
approach,
novel
that
integrates
100-dimensional
features
from
properties-based,
sequence-based,
structure-based
features,
employing
XGBoost
algorithm
prediction.
To
address
unbalanced
datasets,
we
implemented
synthetic
minority
oversampling
technique
(SMOTE).
LMFE
was
validated
across
benchmark
cross-species
independent
datasets.
Results:
achieved
accuracy
of
99.42%,
F1score
0.99,
MCC
0.98
dataset,
with
robust
performance
(accuracy
ranging
89.30%
99.81%).
On
attained
average
99.41%,
representing
12.29%
improvement
over
traditional
methods
without
SMOTE
(average
ACC
87.12%).
Compared
state-of-the-art
methods,
CPC2
PLEKv2,
consistently
outperformed
them
multiple
metrics
datasets
(with
97.33%
99.21%),
redundant
having
minimal
impact
performance.
Conclusions:
provides
highly
generalizable
solution
prediction,
outperforming
existing
through
fusion
while
demonstrating
robustness
features.
Despite
its
effectiveness,
variations
species
highlight
necessity
future
improvements
managing
diverse
genomes.
represents
valuable
tool
advancing
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Mitochondria
are
essential
for
maintaining
health,
and
dysfunction
of
them
leads
to
various
diseases.
Their
role
is
not
limited
energy
production
but
serves
multiple
mechanisms
varying
from
calcium
hemostasis,
reactive
oxygen
species
production,
regulation
apoptotic
cell
death.
In
recent
years,
several
strategies
have
been
developed
preserve
mitochondria.
Ellagic
acid
(EA)
a
polyphenol
extracted
many
plants.
The
intestinal
microflora
converts
EA
urolithins
with
high
bioavailability.
exhibit
mitochondrial‐protective
effects
by
regulating
mitochondrial
complexes,
sirtuins,
mitophagy,
antioxidant
enzymes.
This
review
highlights
the
mito‐protective
on
injuries
induced
drugs
toxic
compounds.
